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The map label for this gene is lpd [H]

Identifier: 126696821

GI number: 126696821

Start: 1251271

End: 1252710

Strand: Reverse

Name: lpd [H]

Synonym: P9301_14831

Alternate gene names: 126696821

Gene position: 1252710-1251271 (Counterclockwise)

Preceding gene: 126696822

Following gene: 126696820

Centisome position: 76.3

GC content: 35.97

Gene sequence:

>1440_bases
GTGACTGATTCAAGTTTTGATTTCGATTTAATTGTAATAGGAGCAGGATATGGAGGTTTTGATGCCGCTAAACATGCTGC
TGGCAAGGGACTGAAAGTCGCAATAGTAGAATCTTCTGATATGGGAGGTACTTGTGTTAACAAGGGTTGTGTTCCATCTA
AAGCTCTTTTGGCTGCAAGTGGAAAAGTTAGAGAAATAGCTGATTATGAACATTTAGCTAAATTTGGTATTCATGCTTCT
CCAGTAAGGTTCGAGAGATCAAAAATTGCAGATCATGCAAATAATTTAGTCTTAAATGTTAGAGAAAATTTAACAAAAAC
TCTTAAAAGAAGTGGAGTTGAAATTATTTTGGGAATTGGAAGAATTGAAGGAAATCAAAAAGTAGGTGTAAGAGATAAAA
ACGGAATTGATAAAATTTTTACATGTAAGAATATTGTTATAGCAACAGGTTCTTCTCCTTTTGTGCCCCGTGGAATAACT
TTGGATAATAGGACCGTATTCACTAGTGATGATGCGGTTAAACTTGAGTGGCTTCCAAGATGGATAGCAATTATTGGAAG
TGGATATATAGGACTAGAATTTGCTGATGTTTATACCGCGCTTGGTTGTGAAGTTACCATGATCGAGGCTTTGGAAAATA
TTATGCCAACATTTGATCCAGACATCACTAAAATTGCTAAGAAGAACCTTATTCAAGCAAGAGATATAGATACAAAATCA
AATGTCTTTGCGACAAAAATAATACCTGGATGTCCTGTAAAAATAGAACTGACGGATGCAAAATCTAAGGAAGTTGTAGA
AACTTTAGAAGTTGACGCTGTACTAGTTGCTACTGGCAGAAGTCCTAATAGTAATAACTTAAATCTTGAGTCGGTTGGTA
TCGAAACAGTAAAAGGGTTTATTCCTATAGATGATCAGATGAGAGTTAAGAATGGTGATGAAATAATACCTAACATTTGG
GCCGTTGGAGATGTAACGGGCAAACTAATGCTTGCCCATACAGCTGCAGCGCAGGGTACTGTTGCTGTTGATAATATTTG
CGGTGGTAATGTCGGAATTAACTATAAAAGTATACCTGCAGCAACCTTTACTCACCCAGAGATAAGTTCAGTTGGTCTCT
CAGAAGCTGAAGCTGAGGAGATATCTGCAAAAGAAAATTTTACTTTGGGAGTTGTCAAAAGTTTCTTTAAGGCTAATTCA
AAAGCATTGGCTGAATTGGAGAGTGACGGATTGCTAAAGTTGATTTTCAACAAAGATAATGGGAAAGTATTAGGTGCTCA
TATTTTTGGGTTACATGCAGCTGATTTAATTCAAGAAATTTCGAACGCTATTTCAAGGAACCAAGATGTAATTCAATTAT
CTAAAGAAGTTCATACTCATCCTACTCTTAGTGAAGTAGTTGAGGTCGCATATAAACAAGCAGCTTCTCAAATAAAATAA

Upstream 100 bases:

>100_bases
CATAGTGAGCTTGTAGAATCATTGAATGTGGCTTGTGTTGCAGTTCCATTATTACTAGAACGAAAAAGAGTCGCATACAC
CTCTAATAAATAAATAAAAA

Downstream 100 bases:

>100_bases
TTTCTAAAATTAATGGAGATAAGACGCAGGCCACCAAATCCAACAGTAAGGGTAGAAAATTTAGAATATGCTGTACCTCA
TAGAGAAGCACAAGCAAAAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAASGKVREIADYEHLAKFGIHAS
PVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIGRIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGIT
LDNRTVFTSDDAVKLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
NVFATKIIPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESVGIETVKGFIPIDDQMRVKNGDEIIPNIW
AVGDVTGKLMLAHTAAAQGTVAVDNICGGNVGINYKSIPAATFTHPEISSVGLSEAEAEEISAKENFTLGVVKSFFKANS
KALAELESDGLLKLIFNKDNGKVLGAHIFGLHAADLIQEISNAISRNQDVIQLSKEVHTHPTLSEVVEVAYKQAASQIK

Sequences:

>Translated_479_residues
MTDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAASGKVREIADYEHLAKFGIHAS
PVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIGRIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGIT
LDNRTVFTSDDAVKLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
NVFATKIIPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESVGIETVKGFIPIDDQMRVKNGDEIIPNIW
AVGDVTGKLMLAHTAAAQGTVAVDNICGGNVGINYKSIPAATFTHPEISSVGLSEAEAEEISAKENFTLGVVKSFFKANS
KALAELESDGLLKLIFNKDNGKVLGAHIFGLHAADLIQEISNAISRNQDVIQLSKEVHTHPTLSEVVEVAYKQAASQIK
>Mature_478_residues
TDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAASGKVREIADYEHLAKFGIHASP
VRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIGRIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGITL
DNRTVFTSDDAVKLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKSN
VFATKIIPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESVGIETVKGFIPIDDQMRVKNGDEIIPNIWA
VGDVTGKLMLAHTAAAQGTVAVDNICGGNVGINYKSIPAATFTHPEISSVGLSEAEAEEISAKENFTLGVVKSFFKANSK
ALAELESDGLLKLIFNKDNGKVLGAHIFGLHAADLIQEISNAISRNQDVIQLSKEVHTHPTLSEVVEVAYKQAASQIK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=35.3556485355649, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=30.2771855010661, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI291045266, Length=485, Percent_Identity=29.0721649484536, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI33519430, Length=483, Percent_Identity=27.1221532091097, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519428, Length=483, Percent_Identity=27.1221532091097, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519426, Length=483, Percent_Identity=27.1221532091097, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI148277065, Length=483, Percent_Identity=27.1221532091097, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI148277071, Length=482, Percent_Identity=26.9709543568465, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=27.5210084033613, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI291045268, Length=483, Percent_Identity=26.7080745341615, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=33.0453563714903, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=27.5789473684211, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789915, Length=452, Percent_Identity=28.7610619469027, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=28.2051282051282, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32565766, Length=485, Percent_Identity=32.9896907216495, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=31.2633832976445, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=31.2633832976445, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17557007, Length=492, Percent_Identity=28.6585365853659, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=491, Percent_Identity=28.3095723014257, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=33.5403726708075, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6325166, Length=486, Percent_Identity=28.3950617283951, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=490, Percent_Identity=27.9591836734694, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358499, Length=478, Percent_Identity=35.3556485355649, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24640553, Length=497, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24640549, Length=495, Percent_Identity=28.6868686868687, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=28.5420944558522, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17737741, Length=502, Percent_Identity=25.8964143426295, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51322; Mature: 51191

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAAS
CCCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GKVREIADYEHLAKFGIHASPVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIG
CCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEEEEEE
RIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGITLDNRTVFTSDDAVKLEWLPR
EECCCCEECCCCCCCCCCEEEECEEEEECCCCCCCCCCEEECCCEEEECCCCEEEEHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
HHHHHCCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCCC
NVFATKIIPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESVGIETVKGF
CEEEEEECCCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHCCE
IPIDDQMRVKNGDEIIPNIWAVGDVTGKLMLAHTAAAQGTVAVDNICGGNVGINYKSIPA
ECCCCCEEECCCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEECCCCCCCCCCEECCCC
ATFTHPEISSVGLSEAEAEEISAKENFTLGVVKSFFKANSKALAELESDGLLKLIFNKDN
CEECCCCCHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCC
GKVLGAHIFGLHAADLIQEISNAISRNQDVIQLSKEVHTHPTLSEVVEVAYKQAASQIK
CEEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAAS
CCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GKVREIADYEHLAKFGIHASPVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIG
CCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEEEEEE
RIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGITLDNRTVFTSDDAVKLEWLPR
EECCCCEECCCCCCCCCCEEEECEEEEECCCCCCCCCCEEECCCEEEECCCCEEEEHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
HHHHHCCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCCC
NVFATKIIPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESVGIETVKGF
CEEEEEECCCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHCCE
IPIDDQMRVKNGDEIIPNIWAVGDVTGKLMLAHTAAAQGTVAVDNICGGNVGINYKSIPA
ECCCCCEEECCCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEECCCCCCCCCCEECCCC
ATFTHPEISSVGLSEAEAEEISAKENFTLGVVKSFFKANSKALAELESDGLLKLIFNKDN
CEECCCCCHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCC
GKVLGAHIFGLHAADLIQEISNAISRNQDVIQLSKEVHTHPTLSEVVEVAYKQAASQIK
CEEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]