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The map label for this gene is trmD [H]

Identifier: 126696803

GI number: 126696803

Start: 1232948

End: 1234168

Strand: Reverse

Name: trmD [H]

Synonym: P9301_14651

Alternate gene names: 126696803

Gene position: 1234168-1232948 (Counterclockwise)

Preceding gene: 126696804

Following gene: 126696802

Centisome position: 75.17

GC content: 32.6

Gene sequence:

>1221_bases
ATGAGTGGTTTTAATTTTGATGTAATTACATTATTCCCTAAAGCTTTTGAATTGATAAATAATTTAGGGGTCATAACAAA
AGCTCTAGATAAGAATTTGATCGATTTAAATTTACATGATTTGAGAGAATATGGAGAAGGTTCTTACAGACAAGTAGACG
ATAAGCCTTATGGAGGAGGAGCAGGTATGGTATTAAAACCTGAACCTATTTATAAGGCATATGAATCAATTAGAAAATTA
CCCAAAAGTAAAACTTTGCTGATGACCCCACAGGGTAAAGTACTAAAGCAAAAGGATCTTGCGAGATGGTCCACTTTGGA
TCAAATAATAATTATTTGTGGTCAATATGAAGGTTTTGATGAAAGGATTAGATGTTTAGCTGATGAAGAGATATCGATAG
GCGATTATGTACTTTCTGGAGGTGAAATACCCGCTATATCAATAATTAATGGTTTGACTAGATTATTACCAGGAACTCTT
GGTGATCCAGATTCCTTAGTAGATGAGAGTCATAATTCTTCTTTATTAGAATATCCTCAATACACAAGGCCGATAACTTT
TAAAGATATGAAAGTGCCAGATATTCTAGTGAGCGGTAATCATGAAGAGATTAAATCGTGGAGAAGAAGAAAAAGTTTTG
AAAGAACATTGGAGAGAAGAAGTGACTTAATTTCAAATGAAAACTACAAAAAATCCCCACAAAGTAAGAGAATAATAAAA
GAAAATAATCAATTCATGAAATTTAGAATAGGCAATGGATACGATATTCACAGACTTGTAGAGGATAGAGATTTAATTAT
TGGAGGTGTAAAATTACATCACCCTGAAAGTTTAGGATTGGATGGGCATAGTGATGCTGATGTTTTAAGTCACTCAATAA
TGGATGCTTTATTGGGAGCTCTTTCGTTGGGCGACATAGGAAAGTATTTCCCCCCATCTGATGAAAAATGGAAAAATGCT
GATAGCTTGTTTTTGTTATCAAAAGTAATTGACTTAATAAGACAAGATGGTTGGGAAATAAATAATATTGATAGCGTTCT
TGTTGCTGAAAGGCCAAAAATCATGCCACATATAAAACTAATGAAAAAAAATATTTCTGAAATTTTAAATATTGATGAAA
ATTTAATTGGGATTAAAGCAACTACGAATGAAAAATTGGGTCCAGAGGGGAGAGAAGAGGGTATAAGTTGCCATTCAGTA
GTTCTTCTTGAAAAAAAATGA

Upstream 100 bases:

>100_bases
TGATTGTCCAGTTCACTTAGAGTTGTTTGTGAAAGTTATTCCAAATTGGAGAAAAAAAGAATCAAGGTTAATTGAGTTTG
GTTATGAGGAAGATTTCTAG

Downstream 100 bases:

>100_bases
GATTTAATTTAAATTTGAAAAAAAAAATTCAAGGATTTTGTCTATCATTAATTTGCTTAGTAGTTTTAACTGTTCAATCT
GATATTCCCAATTTAAGAGC

Product: putative bifunctional enzyme: tRNA methyltransferase; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSGFNFDVITLFPKAFELINNLGVITKALDKNLIDLNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPEPIYKAYESIRKL
PKSKTLLMTPQGKVLKQKDLARWSTLDQIIIICGQYEGFDERIRCLADEEISIGDYVLSGGEIPAISIINGLTRLLPGTL
GDPDSLVDESHNSSLLEYPQYTRPITFKDMKVPDILVSGNHEEIKSWRRRKSFERTLERRSDLISNENYKKSPQSKRIIK
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLHHPESLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDEKWKNA
DSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHIKLMKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSV
VLLEKK

Sequences:

>Translated_406_residues
MSGFNFDVITLFPKAFELINNLGVITKALDKNLIDLNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPEPIYKAYESIRKL
PKSKTLLMTPQGKVLKQKDLARWSTLDQIIIICGQYEGFDERIRCLADEEISIGDYVLSGGEIPAISIINGLTRLLPGTL
GDPDSLVDESHNSSLLEYPQYTRPITFKDMKVPDILVSGNHEEIKSWRRRKSFERTLERRSDLISNENYKKSPQSKRIIK
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLHHPESLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDEKWKNA
DSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHIKLMKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSV
VLLEKK
>Mature_405_residues
SGFNFDVITLFPKAFELINNLGVITKALDKNLIDLNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPEPIYKAYESIRKLP
KSKTLLMTPQGKVLKQKDLARWSTLDQIIIICGQYEGFDERIRCLADEEISIGDYVLSGGEIPAISIINGLTRLLPGTLG
DPDSLVDESHNSSLLEYPQYTRPITFKDMKVPDILVSGNHEEIKSWRRRKSFERTLERRSDLISNENYKKSPQSKRIIKE
NNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLHHPESLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDEKWKNAD
SLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHIKLMKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSVV
LLEKK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=39.2405063291139, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=46.2025316455696, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 45849; Mature: 45718

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFNFDVITLFPKAFELINNLGVITKALDKNLIDLNLHDLREYGEGSYRQVDDKPYGGG
CCCCCEEHEEHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCEECCCCCCCCC
AGMVLKPEPIYKAYESIRKLPKSKTLLMTPQGKVLKQKDLARWSTLDQIIIICGQYEGFD
CCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCHH
ERIRCLADEEISIGDYVLSGGEIPAISIINGLTRLLPGTLGDPDSLVDESHNSSLLEYPQ
HHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCC
YTRPITFKDMKVPDILVSGNHEEIKSWRRRKSFERTLERRSDLISNENYKKSPQSKRIIK
CCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLHHPESLGLDGHSDADVLSHSIMDALLGA
CCCCEEEEECCCCCCHHHHHCCCCEEEECEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDEKWKNADSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHIKL
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHH
MKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSVVLLEKK
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECC
>Mature Secondary Structure 
SGFNFDVITLFPKAFELINNLGVITKALDKNLIDLNLHDLREYGEGSYRQVDDKPYGGG
CCCCEEHEEHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCEECCCCCCCCC
AGMVLKPEPIYKAYESIRKLPKSKTLLMTPQGKVLKQKDLARWSTLDQIIIICGQYEGFD
CCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCHH
ERIRCLADEEISIGDYVLSGGEIPAISIINGLTRLLPGTLGDPDSLVDESHNSSLLEYPQ
HHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCC
YTRPITFKDMKVPDILVSGNHEEIKSWRRRKSFERTLERRSDLISNENYKKSPQSKRIIK
CCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLHHPESLGLDGHSDADVLSHSIMDALLGA
CCCCEEEEECCCCCCHHHHHCCCCEEEECEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDEKWKNADSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHIKL
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHH
MKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSVVLLEKK
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA