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The map label for this gene is glnA [H]

Identifier: 126696277

GI number: 126696277

Start: 806913

End: 808334

Strand: Reverse

Name: glnA [H]

Synonym: P9301_09391

Alternate gene names: 126696277

Gene position: 808334-806913 (Counterclockwise)

Preceding gene: 126696279

Following gene: 126696276

Centisome position: 49.23

GC content: 36.71

Gene sequence:

>1422_bases
ATGTCTAAATCCCCTCAAGATGTTTTAAGTCAAATTAAAGACGAAGGAATTGAACTCATCGATTTAAAATTCACTGATAT
TCATGGAAAATGGCAACATTTAACCCTTACCTCAGACATGATAGAGGAGGATTCTTTTACAGAAGGTTTGGCATTTGATG
GCTCATCAATAAGAGGTTGGAAAGCAATTAATGCCTCGGATATGTCAATGGTTCCCGATGCAAGTACAGCTTGGATAGAT
CCTTTTTATAAACATAAAACTTTAAGTATGATTTGCTCTATTCAAGAGCCAAGAAGCGGGGAACCTTATGATAGGTGTCC
AAGAGCATTAGCTCAAAAGGCTTTAAAATATTTAGACTCGACTGGTATAGCAGATACTGCATTTTTTGGACCAGAACCAG
AATTCTTTTTATTTGATGATGTTAGATATGACTCTAAAGAAGGAGGTTGTTTTTATAGTGTAGATACTATTGAAGCTCCA
TGGAATACAGGGAGAATTGAAGAAGGGGGAAACCTTGGATACAAAATACAATATAAAGAAGGATATTTTCCAGTAGCTCC
AAATGATACTGCGCAAGATATCAGATCTGAGATGCTACTTCTTATGGGTGAATTAGGTATCCCCACCGAAAAACATCACC
ATGAAGTTGCTGGTGCAGGCCAACACGAGCTTGGAATGAAATTTGATTCGTTAATAAATGCTGCTGATAACGTTATGACT
TATAAATACGTTGTCAGAAATGTTGCAAAAAAATATGGAAAAACAGCAACATTTATGCCAAAACCTGTTTTTAACGATAA
CGGAACAGGAATGCATGCTCACCAAAGTTTATGGAAGAGTGGACAGCCACTATTCTTTGGTGAAGGATCATATGCAAATT
TATCTCAAACAGCAAGATGGTACATCGGAGGCATACTTAAACATGCGCCATCATTCTTAGCATTTACTAACCCAACTACT
AATAGTTATAAACGATTGGTTCCAGGATTCGAAGCACCAGTAAATCTAGTTTATTCTGAGGGTAATAGATCAGCTGCGGT
AAGAATACCCTTAACAGGACCAAGCCCCAAAGCTAAGAGATTAGAATTCAGATCAGGTGACGCACTTGCAAATCCTTACT
TAGCATTCTCTGTAATGATGCTTGCTGGTATTGATGGAATTAAAAATCAAATTGACCCTGGTGATGGAGTAGATGTAGAT
TTATTTGAACTTCCAGCTGATGAACTTGCAAAAATCGATACAGTCCCTTCATCTCTAAATGATTCACTTAATGCGCTAAA
AGCAGATAAGGATTATCTATTAGCTGGTGGAGTATTTACTGAAGATTTTATTGATAACTTTATCGATATAAAATACGAAG
AGGTACAACAACTAAGACAAAGGCCTCATCCACATGAATTCTTCATGTATTACGATGCATAA

Upstream 100 bases:

>100_bases
TTGAAACACTAAAAGAAGACATCAAGTCTTAAAAAAAGTTACCGTTGATACATAACAAGAATCATCAAGTTATTAATTTC
ATATAAGGTATTACAAAACT

Downstream 100 bases:

>100_bases
ATAAAAGAAAACAATATTTTAAATTAATCAATTTGAGAAATATCACAAAATATTCTCAAATTGATTTTTTTTTTGTTGGA
ATATACATATATAAATCGAA

Product: glutamine synthetase, glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGWKAINASDMSMVPDASTAWID
PFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDSTGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAP
WNTGRIEEGGNLGYKIQYKEGYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLINAADNVMT
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHAHQSLWKSGQPLFFGEGSYANLSQTARWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVD
LFELPADELAKIDTVPSSLNDSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA

Sequences:

>Translated_473_residues
MSKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGWKAINASDMSMVPDASTAWID
PFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDSTGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAP
WNTGRIEEGGNLGYKIQYKEGYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLINAADNVMT
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHAHQSLWKSGQPLFFGEGSYANLSQTARWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVD
LFELPADELAKIDTVPSSLNDSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA
>Mature_472_residues
SKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGWKAINASDMSMVPDASTAWIDP
FYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDSTGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAPW
NTGRIEEGGNLGYKIQYKEGYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLINAADNVMTY
KYVVRNVAKKYGKTATFMPKPVFNDNGTGMHAHQSLWKSGQPLFFGEGSYANLSQTARWYIGGILKHAPSFLAFTNPTTN
SYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVDL
FELPADELAKIDTVPSSLNDSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=494, Percent_Identity=23.4817813765182, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=53.4188034188034, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI87081870, Length=176, Percent_Identity=30.6818181818182, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52724; Mature: 52593

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGW
CCCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECHHHHCCCCCCCCEEECCCCCCCE
KAINASDMSMVPDASTAWIDPFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDS
EEECCCCCCCCCCCCCHHCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHC
TGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAPWNTGRIEEGGNLGYKIQYKE
CCCCCCCCCCCCCCEEEEECCEECCCCCCEEEEECCEECCCCCCCCCCCCCCCEEEEECC
GYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLINAADNVMT
CEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHH
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHAHQSLWKSGQPLFFGEGSYANLSQTARW
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKR
HHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHE
LEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVDLFELPADELAKIDTVPSSLN
EEECCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCEEEHEECCHHHHHHHHCCCHHHH
DSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA
HHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
SKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGW
CCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECHHHHCCCCCCCCEEECCCCCCCE
KAINASDMSMVPDASTAWIDPFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDS
EEECCCCCCCCCCCCCHHCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHC
TGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAPWNTGRIEEGGNLGYKIQYKE
CCCCCCCCCCCCCCEEEEECCEECCCCCCEEEEECCEECCCCCCCCCCCCCCCEEEEECC
GYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLINAADNVMT
CEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHH
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHAHQSLWKSGQPLFFGEGSYANLSQTARW
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKR
HHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHE
LEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVDLFELPADELAKIDTVPSSLN
EEECCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCEEEHEECCHHHHHHHHCCCHHHH
DSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA
HHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7727755; 8905231 [H]