Click here to switch to the map view.

The map label for this gene is proB

Identifier: 126696187

GI number: 126696187

Start: 737026

End: 738108

Strand: Reverse

Name: proB

Synonym: P9301_08491

Alternate gene names: 126696187

Gene position: 738108-737026 (Counterclockwise)

Preceding gene: 126696188

Following gene: 126696186

Centisome position: 44.96

GC content: 29.55

Gene sequence:

>1083_bases
ATGAAAACTTGGGTTATAAAAATTGGTACTAGTATTTTAAGAGGAACAGAGGAAACATCTACAGAAGAAGTTATTGAAAA
CCTTTCCAGATCCTTTACAAGTTTTCTTTCAAAAGGAAACAAGTTAATTTTAGTAACTAGTGGAGCCGTTGGATTAGGTT
GCCAAAAGTTAAATATTAAAACAAGACCAAATGATTTAAGTACCCTTCAAGCTACTGCTGCAGTAGGTCAAGTTAATTTA
ATGTCCTTATACGATAAAGTATTTAATAAATTAGGTCTTAATATTGCTCAAATTTTAATAACTAAAGCTGATTTTAATTC
ACGAGAATCCTTTAATAACGCTTCTAAAACTTTAAAAAGATTAATCGATTTGAATGTTATTCCAATAGTAAATGAAAATG
ATACCGTAGCAAATGAAGAGCTTAAATATGGAGATAATGATACCCTCTCTGCTTTAGTTGCCTTGGCTATAAATGCTAAC
AAGCTTATTTTATTAACCGATATTGAAAATCTATACTCAAAAGATCCACGTAATAATAAAGATGCGCAACCTATTAAAGA
AGTTCATAATAGTGAATTAAAGGAAATTAAAGATAAAAATATTCAAAACTCAAATAATGAATGGGGAACAGGAGGAATTT
CTACAAAATTAATTTCTGCAGAAATAGCAACAAAAGGAGGAGTCGAAGTCCAACTAGTTGATGGAACTAATAAAAAAAAC
TTAATTGAAATATTTAATGATAATAAAATTGGAACTTTATTTTATCCAGTAGAAAAACCTATTGGAAACAAAAAAAGTTG
GCTTTCACACGCAATTCAAACAGTAGGGAAAATTACTTTAGATGATGGCGCTTCTTTTGCAATTAAAAAAAAAGGTGCCT
CACTTTTAGCGGTTGGTGTTAAGAATGTAGAAGGAAACTTTACGATTAATCAGGCAGTTAAAATCGTAAATACAAATGAT
AAAGAAGTTGCAAAAGGTTTAGTATCAATAAGTAGCGACAAATTAAGAAGTATCTTAAATAATAAAGAAAATAACAACTC
CTCGATAATTGTCGTTCATAGAGATGTTCTTGCTCTCTCTTAG

Upstream 100 bases:

>100_bases
ATATAAAAACAATATTAGTTAAGAGATTAAATAGAGATGGCCTGCCTATAAAATTTAATTTAACTTTGACAATAGAAAAA
TTAATTTCTCATTTTATAAA

Downstream 100 bases:

>100_bases
AAAAAATTAAAACTGAAAAATGCTTTTAAGTGATTTAGTTGATCTAATAAAAAAAGGAAATTCAAATTTTATTAGTGCCA
ATATTTTCGAAGATTTAAAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKVFNKLGLNIAQILITKADFNSRESFNNASKTLKRLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGVKNVEGNFTINQAVKIVNTND
KEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS

Sequences:

>Translated_360_residues
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKVFNKLGLNIAQILITKADFNSRESFNNASKTLKRLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGVKNVEGNFTINQAVKIVNTND
KEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS
>Mature_360_residues
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKVFNKLGLNIAQILITKADFNSRESFNNASKTLKRLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGVKNVEGNFTINQAVKIVNTND
KEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=29.9319727891156, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=29.7297297297297, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=37.1900826446281, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=312, Percent_Identity=28.2051282051282, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=312, Percent_Identity=28.2051282051282, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=354, Percent_Identity=32.2033898305085, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=232, Percent_Identity=42.2413793103448, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=32.9896907216495, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_PROM0 (A3PCJ7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091073.1
- ProteinModelPortal:   A3PCJ7
- SMR:   A3PCJ7
- STRING:   A3PCJ7
- GeneID:   4911205
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_08491
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   PMAR167546:P9301ORF_0865-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39242; Mature: 39242

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIK
CCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
TRPNDLSTLQATAAVGQVNLMSLYDKVFNKLGLNIAQILITKADFNSRESFNNASKTLKR
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEHHEEEECCCCCCHHHHHHHHHHHH
LIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRNNK
HHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCEEEEECCCCCCC
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGV
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCEEEECCCCCEEEECCCCEEEEEEE
KNVEGNFTINQAVKIVNTNDKEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS
ECCCCCEEEEHEEEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEECCEEECC
>Mature Secondary Structure
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIK
CCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
TRPNDLSTLQATAAVGQVNLMSLYDKVFNKLGLNIAQILITKADFNSRESFNNASKTLKR
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEHHEEEECCCCCCHHHHHHHHHHHH
LIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRNNK
HHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCEEEEECCCCCCC
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGV
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCEEEECCCCCEEEECCCCEEEEEEE
KNVEGNFTINQAVKIVNTNDKEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS
ECCCCCEEEEHEEEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA