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The map label for this gene is leuB [H]

Identifier: 126696185

GI number: 126696185

Start: 734870

End: 735943

Strand: Reverse

Name: leuB [H]

Synonym: P9301_08471

Alternate gene names: 126696185

Gene position: 735943-734870 (Counterclockwise)

Preceding gene: 126696186

Following gene: 126696184

Centisome position: 44.82

GC content: 31.66

Gene sequence:

>1074_bases
ATGAAGAAATATAAGATTGTTTTATTATCAGGAGACGGAATTGGACCAGAGATTTCAGAAGTTTCAAAAAAAGTTTTAAA
AAAGCTTTCAAAAAATCATAACTTCGATATTGAGATTATTGAAAAATTATTTGGAGGGATAGCGTATGAAAAATATGGTA
CTCCTGCTCCAGATGAGACACTAGATCAATGTAAAAAAAGTGACGCAGTACTTTTAGCATGTGTTGGAGATATTAAATAT
GATTCTCTTGCAAGAGAATTAAGGCCAGAAAGTGGGTTACTAAAGTTAAGATCTGCCCTAAACCTTTTCGCAAATATCAG
GCCTGTCAAAATAAGAAAATCTCTATTAGATGCAAGTTCATTAAAAAAAGAAATCGTTGAGCATGTAGATCTTATTGTCG
TGAGAGAATTAATAGGAGGAATTTATTTTGGAAAACCAAGAGGCCACATAACAAATACAAAAATACCAAAAGCTTTCAAT
ACTATGGTTTATGATTCGAATGAAATAGAAAGAATAACCGAAATAGCAATAAAAATTGCTAACCAAAGAAATAAAAAAAT
ATGTTCTGTTGATAAATCAAACGTTCTTGAGGTTAGTCAATTGTGGAGAGATACAGTTTTAAATATCACCTCAAAAGACA
AAAATATATCTCTAAGCAATATGTACGTTGATAATGCAGCAATGCAATTAGTTAGAGATCCTGGTCAATTTGATGTGATT
TTAACTAGTAATTTATTTGGAGATATTTTAAGCGACTTAGCCGCAATGTTAACTGGTTCTATTGGTATGCTTCCATCTGC
TTCTTTAAACAATAATGGCCCAGGCGTTTTTGAACCTGTTCATGGTTCGGCTCCTGATATAGCTGGTAAAAACATAGCTA
ATCCTATTGCAATGCTTTTATCTGCATCCATGATGTTAAAAATTGGATTAAATGAAGAAGAAGCTGCAGAAAATTTAGAA
ACTGCCATTGATAAAGTTTTATCAAAAGGATTTAGAACAGCAGATTTAGCTGATGGGTCTACTGAAGTTTTATCTTGCAG
TGAAATCGGAGATAAAATAATTAATGAAATTTAA

Upstream 100 bases:

>100_bases
CTAAGGAGTTCAAGTATTTTTAAAAAATTACCAGAATTAGCAAAAAAACTTAGACAATTAGACAAGCAATAATTTATTGT
TCTATTAAACAAAAATTTAA

Downstream 100 bases:

>100_bases
AAAAAAATTAATAAATAAAAAAATATTTTTAAGTAAAACATAAAGTTGGCATATGACCTGTCAGAATCAATAGATAATAT
TTTTTAAAAATGTCAAAACG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDETLDQCKKSDAVLLACVGDIKY
DSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLDASSLKKEIVEHVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDSNEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAENLE
TAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIINEI

Sequences:

>Translated_357_residues
MKKYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDETLDQCKKSDAVLLACVGDIKY
DSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLDASSLKKEIVEHVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDSNEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAENLE
TAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIINEI
>Mature_357_residues
MKKYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDETLDQCKKSDAVLLACVGDIKY
DSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLDASSLKKEIVEHVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDSNEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAENLE
TAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIINEI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=357, Percent_Identity=31.6526610644258, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI28178816, Length=362, Percent_Identity=29.2817679558011, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178821, Length=314, Percent_Identity=30.5732484076433, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4758582, Length=320, Percent_Identity=27.8125, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=27.5541795665635, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=38.0281690140845, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=48.3050847457627, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=32.8571428571429, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787381, Length=407, Percent_Identity=26.2899262899263, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=29.9120234604106, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=28.4916201117318, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=28.0346820809249, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17505779, Length=349, Percent_Identity=28.080229226361, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=45.0819672131148, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=36.7688022284123, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=31.6091954022989, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=364, Percent_Identity=29.6703296703297, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24643270, Length=355, Percent_Identity=32.112676056338, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643268, Length=355, Percent_Identity=32.112676056338, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24661184, Length=367, Percent_Identity=31.3351498637602, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI161078633, Length=349, Percent_Identity=27.5071633237822, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650122, Length=349, Percent_Identity=27.5071633237822, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078635, Length=349, Percent_Identity=27.5071633237822, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=349, Percent_Identity=27.5071633237822, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=29.6829971181556, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=29.6829971181556, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078639, Length=344, Percent_Identity=27.906976744186, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI20130355, Length=226, Percent_Identity=26.1061946902655, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38988; Mature: 38988

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDET
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHCCCCCCHHH
LDQCKKSDAVLLACVGDIKYDSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLDASS
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIVEHVDLIVVRELIGGIYFGKPRGHITNTKIPKAFNTMVYDSNEIERITEIAIKIA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHEECCHHHHHHHHHHHHHH
NQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
HCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHCCCCCEEEE
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SASMMLKIGLNEEEAAENLETAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIINEI
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEHHHHHHHHHHCC
>Mature Secondary Structure
MKKYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDET
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHCCCCCCHHH
LDQCKKSDAVLLACVGDIKYDSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLDASS
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIVEHVDLIVVRELIGGIYFGKPRGHITNTKIPKAFNTMVYDSNEIERITEIAIKIA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHEECCHHHHHHHHHHHHHH
NQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
HCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHCCCCCEEEE
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SASMMLKIGLNEEEAAENLETAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIINEI
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA