Click here to switch to the map view.

The map label for this gene is cocE [H]

Identifier: 126695949

GI number: 126695949

Start: 535791

End: 537365

Strand: Reverse

Name: cocE [H]

Synonym: P9301_06111

Alternate gene names: 126695949

Gene position: 537365-535791 (Counterclockwise)

Preceding gene: 126695950

Following gene: 126695948

Centisome position: 32.73

GC content: 34.6

Gene sequence:

>1575_bases
ATGTCTGGTTCAAGATGGTTTGACAAGTCTCTAGTTCTTAGAGATGGAGTAAAACTTATATCAAGGATTTGGTTACCTAA
TAGTAAAGGGCCATGGCCTGCATTATTGATGAGACAACCATATGGAAGGGAAATAGCTTCAACTATTACCTATTCTCACC
CAGAATGGTGGGTTTCCAAAGGGTATATGGTAATAATTCAAGATGTTAGAGGTATGGGTTCTTCAGAAGGAGTTTTTAAT
GGTTTTTCTCAAGAAGCTAGCGATACTTCAGAAACACATGAATGGGTAAGGTCTCTAAAAGAATGTAATGGGAAACTTGG
CTTATATGGTTTTTCATATCAAGGATTGACTCAACTGACTGGTGAATTAAATTCAAAGCCGCCCGATTGCTTATCTCCAG
CAATGACTGGGATGAATATTAAGGACCATTGGTGTTCAGATGGAGGAGCATATTGGTGGCATAACAATATTGCATGGGGA
CTTCAAATTGCAGCACTAAAAATGAAAAGAGAAAATAAATTGCTTGAATGGGAAAAGATAACATTAGCCTTAGAAAATAA
AAGTTATTTAAGGGAAGGAATTGATACGTTAAAAAAATATGATCCTAATAGCTTTGTTTTGGAATGGCTTAAAAATTTAA
ATAATGCTCTCCCATTTGAAGAATTCAAACCAATTTCAACATGGATCAAACAACCTATGTTAATTATTGGAGGACTTTGG
GATCCACATTTAAAAGGTGCCTTTGATCTCTATAAAAAATCTAAAGAAGCTGGTGGAAACCCAGAGATTATTATTGGGAA
TGCGACACATCTAAGTTGGTGGGAGGGATCACAAGAATCTTTATTAAAATTTTTTGATAAGCATTTAAAATCAGATGAGA
AATTAAATTCTACGATTTTTAAACACGAGAAAAAAATATGGAATATTTCATTAAAAAAATGGGAAGAATTAGATAATAAA
TTTCACCCTGAATTTATTTTTGGGCTCAAAAGCGATGGCACAGCAAACGTAGAGGTTGAAGATGGAAATCTGACCATAAA
TTCAAAAGGATCAGGATGGTTCACAATTGTTAATGACCCATGGAGACCTGCTCCATCTGACGGTGGTCATTTAGGCCCTA
ATCCAGGAAACTTTAATAGAAGCATTATTGATAAACGCCTGGATGTAGGTGTTTTTCAAACCAATCCTTTTGAAGAAGAT
CAATATTTAAGAGGAGTTCCCACATTAGAAATCCAAGTAAAAAGTGATCAGCCTAATTTCGATATCTGCCTTGCTTTATC
TCTAGTTGAAGAAGTTAATGAAAAGGTGAATCAATTTTCAACCGGTTTCTTAAGGGTTAAAAACTCCAAAATAAGTGAAG
AATGCATTTATCAAATAACAATGCAGCCAACAAATATTTGTTTGATTAAAAATAGCAAACTTCGCTTATCTATATCTGCC
GCAGCTTATCCCGCTATTGGAGTTAATCCTGGATTTGGGGAGGGAAATGTTGGAGCCCCTTCAGCAAATCATAGAGTTAT
TACTCTAAGTTTTAGCCTTAATAAAACATTTATGAAAATGACTCCTTTTTTTTAA

Upstream 100 bases:

>100_bases
GAGTATGTATCAAATAAAAAAAAGTTAACTCCCGTTAACGCGGAGATCTTTTCTATTTACGTAATTGGGCTGCACTGCCC
AGACATTATCTAAATCCTAA

Downstream 100 bases:

>100_bases
TAAATAATCATTTTTTTGGTTAATCTTTTGATATTATTAATCCTTAACATCTACCAGTCATGATCGAGACAATTCAAGCA
GACTGGATTAAATCAGAAGC

Product: acyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSGSRWFDKSLVLRDGVKLISRIWLPNSKGPWPALLMRQPYGREIASTITYSHPEWWVSKGYMVIIQDVRGMGSSEGVFN
GFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGLTQLTGELNSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWG
LQIAALKMKRENKLLEWEKITLALENKSYLREGIDTLKKYDPNSFVLEWLKNLNNALPFEEFKPISTWIKQPMLIIGGLW
DPHLKGAFDLYKKSKEAGGNPEIIIGNATHLSWWEGSQESLLKFFDKHLKSDEKLNSTIFKHEKKIWNISLKKWEELDNK
FHPEFIFGLKSDGTANVEVEDGNLTINSKGSGWFTIVNDPWRPAPSDGGHLGPNPGNFNRSIIDKRLDVGVFQTNPFEED
QYLRGVPTLEIQVKSDQPNFDICLALSLVEEVNEKVNQFSTGFLRVKNSKISEECIYQITMQPTNICLIKNSKLRLSISA
AAYPAIGVNPGFGEGNVGAPSANHRVITLSFSLNKTFMKMTPFF

Sequences:

>Translated_524_residues
MSGSRWFDKSLVLRDGVKLISRIWLPNSKGPWPALLMRQPYGREIASTITYSHPEWWVSKGYMVIIQDVRGMGSSEGVFN
GFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGLTQLTGELNSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWG
LQIAALKMKRENKLLEWEKITLALENKSYLREGIDTLKKYDPNSFVLEWLKNLNNALPFEEFKPISTWIKQPMLIIGGLW
DPHLKGAFDLYKKSKEAGGNPEIIIGNATHLSWWEGSQESLLKFFDKHLKSDEKLNSTIFKHEKKIWNISLKKWEELDNK
FHPEFIFGLKSDGTANVEVEDGNLTINSKGSGWFTIVNDPWRPAPSDGGHLGPNPGNFNRSIIDKRLDVGVFQTNPFEED
QYLRGVPTLEIQVKSDQPNFDICLALSLVEEVNEKVNQFSTGFLRVKNSKISEECIYQITMQPTNICLIKNSKLRLSISA
AAYPAIGVNPGFGEGNVGAPSANHRVITLSFSLNKTFMKMTPFF
>Mature_523_residues
SGSRWFDKSLVLRDGVKLISRIWLPNSKGPWPALLMRQPYGREIASTITYSHPEWWVSKGYMVIIQDVRGMGSSEGVFNG
FSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGLTQLTGELNSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGL
QIAALKMKRENKLLEWEKITLALENKSYLREGIDTLKKYDPNSFVLEWLKNLNNALPFEEFKPISTWIKQPMLIIGGLWD
PHLKGAFDLYKKSKEAGGNPEIIIGNATHLSWWEGSQESLLKFFDKHLKSDEKLNSTIFKHEKKIWNISLKKWEELDNKF
HPEFIFGLKSDGTANVEVEDGNLTINSKGSGWFTIVNDPWRPAPSDGGHLGPNPGNFNRSIIDKRLDVGVFQTNPFEEDQ
YLRGVPTLEIQVKSDQPNFDICLALSLVEEVNEKVNQFSTGFLRVKNSKISEECIYQITMQPTNICLIKNSKLRLSISAA
AYPAIGVNPGFGEGNVGAPSANHRVITLSFSLNKTFMKMTPFF

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 59352; Mature: 59221

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSRWFDKSLVLRDGVKLISRIWLPNSKGPWPALLMRQPYGREIASTITYSHPEWWVSK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHCC
GYMVIIQDVRGMGSSEGVFNGFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGLTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
GELNSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGLQIAALKMKRENKLLEWEKI
HHCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCEECEEEEEEHHHHCCCCEEEEEE
TLALENKSYLREGIDTLKKYDPNSFVLEWLKNLNNALPFEEFKPISTWIKQPMLIIGGLW
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHCCCEEEEECCC
DPHLKGAFDLYKKSKEAGGNPEIIIGNATHLSWWEGSQESLLKFFDKHLKSDEKLNSTIF
CCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
KHEKKIWNISLKKWEELDNKFHPEFIFGLKSDGTANVEVEDGNLTINSKGSGWFTIVNDP
HHHHHHEEEEHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCEEEECCCCCEEEEEECCC
WRPAPSDGGHLGPNPGNFNRSIIDKRLDVGVFQTNPFEEDQYLRGVPTLEIQVKSDQPNF
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCHHHCCCCEEEEEEECCCCCC
DICLALSLVEEVNEKVNQFSTGFLRVKNSKISEECIYQITMQPTNICLIKNSKLRLSISA
EEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEEEC
AAYPAIGVNPGFGEGNVGAPSANHRVITLSFSLNKTFMKMTPFF
CCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCC
>Mature Secondary Structure 
SGSRWFDKSLVLRDGVKLISRIWLPNSKGPWPALLMRQPYGREIASTITYSHPEWWVSK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHCC
GYMVIIQDVRGMGSSEGVFNGFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGLTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
GELNSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGLQIAALKMKRENKLLEWEKI
HHCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCEECEEEEEEHHHHCCCCEEEEEE
TLALENKSYLREGIDTLKKYDPNSFVLEWLKNLNNALPFEEFKPISTWIKQPMLIIGGLW
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHCCCEEEEECCC
DPHLKGAFDLYKKSKEAGGNPEIIIGNATHLSWWEGSQESLLKFFDKHLKSDEKLNSTIF
CCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
KHEKKIWNISLKKWEELDNKFHPEFIFGLKSDGTANVEVEDGNLTINSKGSGWFTIVNDP
HHHHHHEEEEHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCEEEECCCCCEEEEEECCC
WRPAPSDGGHLGPNPGNFNRSIIDKRLDVGVFQTNPFEEDQYLRGVPTLEIQVKSDQPNF
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCHHHCCCCEEEEEEECCCCCC
DICLALSLVEEVNEKVNQFSTGFLRVKNSKISEECIYQITMQPTNICLIKNSKLRLSISA
EEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEEEC
AAYPAIGVNPGFGEGNVGAPSANHRVITLSFSLNKTFMKMTPFF
CCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]