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The map label for this gene is glgB

Identifier: 126695948

GI number: 126695948

Start: 533467

End: 535731

Strand: Reverse

Name: glgB

Synonym: P9301_06101

Alternate gene names: 126695948

Gene position: 535731-533467 (Counterclockwise)

Preceding gene: 126695949

Following gene: 126695947

Centisome position: 32.63

GC content: 35.72

Gene sequence:

>2265_bases
ATGATCGAGACAATTCAAGCAGACTGGATTAAATCAGAAGCGATCAACTTAGAAAATTGTTGCAATGATAATCCATTAAA
AATATTAGGTCCTCATTTTTACGAAGAACAATGGGTAATAAGGGTATGGGTGCCTGAAGCTGATGAAGTTAAAGTAAATT
TTAAAGATAAGACCTACAAAGCAGAAAGCATAAATCATAAATGGCTTTTTGAAGCAATCTTGCCGGAAAATCCAAATTCT
AATTACGAAATAAATATTTCACGAGGAGGTATCACACATACGCAACATGATCCCTGGTCATATAGAGAAGAGTGGATGGG
AGAAGTTGATAGGCATCTTTTTGCAGAAGGTAATCACCATCATATTTGGGAAAAGATGGGAGCACATCTAATTGAGGACA
AAAATCAAAAAGGTGTCATGTTCTGCATTTGGGCTCCAAATGCAAAATCAATCTCGATAATTGGAGACATAAATTCTTGG
GATGGAAGACATAATCCAATGCAAAAAAGATTAGGGGGGATTTGGGAACTATTCATGCCAATGATGAAAGAAGGAGATAA
ATATAAATATGAAATAAGAACACAACAAGGTCATATTTATGAAAAGGCCGACCCATACGGATTCTTACATGAAGTTAGGC
CTCAAAATGGTTCAATAGTTTCAAAATTGAAAAACTTTAATTGGAGTGATAGTTCTTGGATTTCAAATAGAGATTCTTCT
AGTCAAATTAACAAGCCAATTTCAGTTTATGAAATGCATTTAGGAAGTTGGCTCCATGAATCAACAGATAACAAATATCT
TGATGACAATGGTGAACCAAGAGACCCAGTGCCTGCAGCTGATTTAAAACCTGGAACAAGATTATTAACTTATCCAGAAT
TAACCAAAAAACTTATCCCTTATGTAAAAGAGAGAGGATTCACTCATATTGAACTTATGCCGATATCTGAACATCCTTTC
GATGGTTCATGGGGATATCAGGTTACAGGCTGGTATGCACCAACAAGTAGATTTGGCACCCCAAATGAATTTAGGGAGTT
TGTTAATAAATGCCATGAAGAGGGCATAGGTGTAATTCTTGATTGGGTGCCTGGTCATTTCCCAAAAGATAAGCATGGTT
TAGCATTTTTTGATGGTTGTCATCTTTACGAACATGGGGATTCACGAATAGGTGAACACAAAGAATGGGGAACTTTAATA
TTTAATTACAGCAGAAACGAAGTAAGAAATTTCTTAGTAGCCAATCTCGTTTATTGGTTTGAAGAATTTCATATTGATGG
CATAAGAGTAGACGCTGTAGCTTCAATGTTATATAGAGATTATTTACGCCCAGATGGAGAATGGATACCCAATGAAAATG
GTGGGAATGAAAATATAGAAGCCGTTAAATTTCTTCAACAGGCTAATCATGTACTCTTCCAACATTTCCCAGGTGCACTC
TCTATCGCTGAAGAATCAACAACTTGGCCAATGGTAACCAAACCAACAGATATGGGGGGATTAGGATTTAATCTAAAATG
GAATATGGGATGGATGCATGATATGCTCGACTATTTCGAGATAGATCCTTGGTTTAGGCAATTCCATCAAAATAGTGTAA
CTTTCTCAATAACATATAACTATACAGAGAACTTTATGCTTGCGCTCAGTCATGATGAAGTTGTCCATGGAAAAAGTCAT
CTATTGCACAAAATGCCTGGCGATGACTGGAAGAAATATGCAAATACTCGAGCCTTACTAACTTATATGTGGACCCACCC
TGGTAAAAAAACGATATTTATGGGAATGGAATTTGGACAAAGGCAAGAATGGAATGTTTGGGATGATCTGCAATGGGAAT
TACTAGAATTTGAGCCTCATAGAGGAATAAGAAACTTGATTGATGACTTAAATAAACTATATAAAAATGAACCTGCGTTA
TGGAAAAACGACTTTGATCCTTATGGATTCCAATGGATTGATTGTAATGACAAATCTAATTCGGTAATAAGTTTTATGAG
AAGAGAAAGCGATACCAATGAATGGCTTGTTATTGTTGCCAACTTTACACCTAATACCCATGGTTCATATAAAATAGGCG
TTCCTGTAGAAGGATTCTACAAAGAGATATTTAATTCAGATGGATCAAGATATGGGGGGAGTAACAAAGGAAATATGGGA
GGTAAAGACACTATAAATTACAATATTCATGATTATCAAAATGCTCTAGAACTAGCTTTGCCCCCATTAAGCGTAAGTAT
CTTCAAACATCAATCAGAAAAATAA

Upstream 100 bases:

>100_bases
GCCTTAATAAAACATTTATGAAAATGACTCCTTTTTTTTAATAAATAATCATTTTTTTGGTTAATCTTTTGATATTATTA
ATCCTTAACATCTACCAGTC

Downstream 100 bases:

>100_bases
TAAGGCTAATTTAACTTAGTACTTCATATAAATGTTCACATAACGCTTTTGATATGCTTTGCATTGTAAGATTTCTAAGT
TGGAATTTTTAAAAAATTTT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MIETIQADWIKSEAINLENCCNDNPLKILGPHFYEEQWVIRVWVPEADEVKVNFKDKTYKAESINHKWLFEAILPENPNS
NYEINISRGGITHTQHDPWSYREEWMGEVDRHLFAEGNHHHIWEKMGAHLIEDKNQKGVMFCIWAPNAKSISIIGDINSW
DGRHNPMQKRLGGIWELFMPMMKEGDKYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIVSKLKNFNWSDSSWISNRDSS
SQINKPISVYEMHLGSWLHESTDNKYLDDNGEPRDPVPAADLKPGTRLLTYPELTKKLIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGTPNEFREFVNKCHEEGIGVILDWVPGHFPKDKHGLAFFDGCHLYEHGDSRIGEHKEWGTLI
FNYSRNEVRNFLVANLVYWFEEFHIDGIRVDAVASMLYRDYLRPDGEWIPNENGGNENIEAVKFLQQANHVLFQHFPGAL
SIAEESTTWPMVTKPTDMGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYNYTENFMLALSHDEVVHGKSH
LLHKMPGDDWKKYANTRALLTYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWELLEFEPHRGIRNLIDDLNKLYKNEPAL
WKNDFDPYGFQWIDCNDKSNSVISFMRRESDTNEWLVIVANFTPNTHGSYKIGVPVEGFYKEIFNSDGSRYGGSNKGNMG
GKDTINYNIHDYQNALELALPPLSVSIFKHQSEK

Sequences:

>Translated_754_residues
MIETIQADWIKSEAINLENCCNDNPLKILGPHFYEEQWVIRVWVPEADEVKVNFKDKTYKAESINHKWLFEAILPENPNS
NYEINISRGGITHTQHDPWSYREEWMGEVDRHLFAEGNHHHIWEKMGAHLIEDKNQKGVMFCIWAPNAKSISIIGDINSW
DGRHNPMQKRLGGIWELFMPMMKEGDKYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIVSKLKNFNWSDSSWISNRDSS
SQINKPISVYEMHLGSWLHESTDNKYLDDNGEPRDPVPAADLKPGTRLLTYPELTKKLIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGTPNEFREFVNKCHEEGIGVILDWVPGHFPKDKHGLAFFDGCHLYEHGDSRIGEHKEWGTLI
FNYSRNEVRNFLVANLVYWFEEFHIDGIRVDAVASMLYRDYLRPDGEWIPNENGGNENIEAVKFLQQANHVLFQHFPGAL
SIAEESTTWPMVTKPTDMGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYNYTENFMLALSHDEVVHGKSH
LLHKMPGDDWKKYANTRALLTYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWELLEFEPHRGIRNLIDDLNKLYKNEPAL
WKNDFDPYGFQWIDCNDKSNSVISFMRRESDTNEWLVIVANFTPNTHGSYKIGVPVEGFYKEIFNSDGSRYGGSNKGNMG
GKDTINYNIHDYQNALELALPPLSVSIFKHQSEK
>Mature_754_residues
MIETIQADWIKSEAINLENCCNDNPLKILGPHFYEEQWVIRVWVPEADEVKVNFKDKTYKAESINHKWLFEAILPENPNS
NYEINISRGGITHTQHDPWSYREEWMGEVDRHLFAEGNHHHIWEKMGAHLIEDKNQKGVMFCIWAPNAKSISIIGDINSW
DGRHNPMQKRLGGIWELFMPMMKEGDKYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIVSKLKNFNWSDSSWISNRDSS
SQINKPISVYEMHLGSWLHESTDNKYLDDNGEPRDPVPAADLKPGTRLLTYPELTKKLIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGTPNEFREFVNKCHEEGIGVILDWVPGHFPKDKHGLAFFDGCHLYEHGDSRIGEHKEWGTLI
FNYSRNEVRNFLVANLVYWFEEFHIDGIRVDAVASMLYRDYLRPDGEWIPNENGGNENIEAVKFLQQANHVLFQHFPGAL
SIAEESTTWPMVTKPTDMGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYNYTENFMLALSHDEVVHGKSH
LLHKMPGDDWKKYANTRALLTYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWELLEFEPHRGIRNLIDDLNKLYKNEPAL
WKNDFDPYGFQWIDCNDKSNSVISFMRRESDTNEWLVIVANFTPNTHGSYKIGVPVEGFYKEIFNSDGSRYGGSNKGNMG
GKDTINYNIHDYQNALELALPPLSVSIFKHQSEK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=658, Percent_Identity=27.0516717325228, Blast_Score=200, Evalue=3e-51,
Organism=Escherichia coli, GI1789839, Length=755, Percent_Identity=45.1655629139073, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=638, Percent_Identity=26.1755485893417, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32564391, Length=610, Percent_Identity=25.9016393442623, Blast_Score=152, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6320826, Length=702, Percent_Identity=25.4985754985755, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28573410, Length=718, Percent_Identity=25.2089136490251, Blast_Score=199, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_PROM0 (A3PBV8)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090834.1
- ProteinModelPortal:   A3PBV8
- SMR:   A3PBV8
- STRING:   A3PBV8
- GeneID:   4911365
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_06101
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   PRIRANN
- ProtClustDB:   PRK05402
- BioCyc:   PMAR167546:P9301ORF_0625-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 88130; Mature: 88130

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: ACT_SITE 326-326 ACT_SITE 361-361 ACT_SITE 366-366 ACT_SITE 429-429 ACT_SITE 431-431 ACT_SITE 484-484 ACT_SITE 551-551 ACT_SITE 552-552

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETIQADWIKSEAINLENCCNDNPLKILGPHFYEEQWVIRVWVPEADEVKVNFKDKTYK
CCCCCCCHHHHHHCCCHHHCCCCCCCEEECCCCCCCCEEEEEECCCCCCEEEEECCCEEE
AESINHKWLFEAILPENPNSNYEINISRGGITHTQHDPWSYREEWMGEVDRHLFAEGNHH
CCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
HIWEKMGAHLIEDKNQKGVMFCIWAPNAKSISIIGDINSWDGRHNPMQKRLGGIWELFMP
HHHHHHCHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
MMKEGDKYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIVSKLKNFNWSDSSWISNRDSS
HHCCCCCEEEEEEECCCCEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCH
SQINKPISVYEMHLGSWLHESTDNKYLDDNGEPRDPVPAADLKPGTRLLTYPELTKKLIP
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGTPNEFREFVNKCHEEGIGVIL
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
DWVPGHFPKDKHGLAFFDGCHLYEHGDSRIGEHKEWGTLIFNYSRNEVRNFLVANLVYWF
ECCCCCCCCCCCCEEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EEFHIDGIRVDAVASMLYRDYLRPDGEWIPNENGGNENIEAVKFLQQANHVLFQHFPGAL
HHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWPMVTKPTDMGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYN
EECCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEEE
YTENFMLALSHDEVVHGKSHLLHKMPGDDWKKYANTRALLTYMWTHPGKKTIFMGMEFGQ
CCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCC
RQEWNVWDDLQWELLEFEPHRGIRNLIDDLNKLYKNEPALWKNDFDPYGFQWIDCNDKSN
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
SVISFMRRESDTNEWLVIVANFTPNTHGSYKIGVPVEGFYKEIFNSDGSRYGGSNKGNMG
HHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCC
GKDTINYNIHDYQNALELALPPLSVSIFKHQSEK
CCCEEEECHHHHHHHHHHCCCCCEEEEECCCCCC
>Mature Secondary Structure
MIETIQADWIKSEAINLENCCNDNPLKILGPHFYEEQWVIRVWVPEADEVKVNFKDKTYK
CCCCCCCHHHHHHCCCHHHCCCCCCCEEECCCCCCCCEEEEEECCCCCCEEEEECCCEEE
AESINHKWLFEAILPENPNSNYEINISRGGITHTQHDPWSYREEWMGEVDRHLFAEGNHH
CCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
HIWEKMGAHLIEDKNQKGVMFCIWAPNAKSISIIGDINSWDGRHNPMQKRLGGIWELFMP
HHHHHHCHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
MMKEGDKYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIVSKLKNFNWSDSSWISNRDSS
HHCCCCCEEEEEEECCCCEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCH
SQINKPISVYEMHLGSWLHESTDNKYLDDNGEPRDPVPAADLKPGTRLLTYPELTKKLIP
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGTPNEFREFVNKCHEEGIGVIL
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
DWVPGHFPKDKHGLAFFDGCHLYEHGDSRIGEHKEWGTLIFNYSRNEVRNFLVANLVYWF
ECCCCCCCCCCCCEEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EEFHIDGIRVDAVASMLYRDYLRPDGEWIPNENGGNENIEAVKFLQQANHVLFQHFPGAL
HHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWPMVTKPTDMGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYN
EECCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEEE
YTENFMLALSHDEVVHGKSHLLHKMPGDDWKKYANTRALLTYMWTHPGKKTIFMGMEFGQ
CCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCC
RQEWNVWDDLQWELLEFEPHRGIRNLIDDLNKLYKNEPALWKNDFDPYGFQWIDCNDKSN
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
SVISFMRRESDTNEWLVIVANFTPNTHGSYKIGVPVEGFYKEIFNSDGSRYGGSNKGNMG
HHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCC
GKDTINYNIHDYQNALELALPPLSVSIFKHQSEK
CCCEEEECHHHHHHHHHHCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA