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The map label for this gene is proS

Identifier: 126695873

GI number: 126695873

Start: 465722

End: 467524

Strand: Reverse

Name: proS

Synonym: P9301_05351

Alternate gene names: 126695873

Gene position: 467524-465722 (Counterclockwise)

Preceding gene: 126695879

Following gene: 126695872

Centisome position: 28.47

GC content: 33.11

Gene sequence:

>1803_bases
ATGCGCGTGACAACCTCATTTCCTCTGGGGACACTTCGTGACACACCTTCTGAAGCTGAGATTATTTCACATCAATTACT
CTTAAAAGCTGGGTATATTCGCAGAGTTAACAGTGGTATTTATGCATACATGCCACTAATGCTTAGAGTTGTTGAAAAAA
TATCCGCAATAATAGAGAAAGAACTTAATAGTATTGGTTGTACAAAATTGCTATTACCTCAACTTCATCCTGCAGATTTA
TGGAAAAAAAGTGAAAGGTGGGAAGGATATACCGCAGGTGAAGGAATAATGTTTAATCTCAAAGATAGACAAGGTAAAGA
ATTTGGTTTAGCACCAACTCACGAAGAGGTGATCACGAGTATTGCATCAGAGACCATCAACTCCTATAAGCAATTACCTC
AATGTTTTTACCAAATTCAGACAAAATTTAGAGATGAAATAAGGCCAAGGTTTGGATTGATGAGAAGTAGAGAATTCATA
ATGAAAGATGGCTATTCTTTTCATTCTTCAGAAAACGATCTAGCTTCTTTCTATGAAAAGGTGGGCAATGCTTATGAAAA
TATTTTTGAATCTTGTGGACTGCAGACAGTAGGGGTTGATGCTGATAGTGGAGCAATTGGCGGTGCTTCATCTAAAGAAT
TTATGGTCACTGCTGATGCTGGGGAAGATTCTATTTTGTTCACTCAAAGTGGTTCTTATGCTGCCAATATCGAAAAAGCT
GTTTCTCTACCCTCTCAACCTATTCCACTAAAAGATAATATTGCAGAGTGGTTGGAAACGCCTAATCAAAAAACAATCCA
AGAAGTTTGTGATAATAACAATTTAGACCCAAGTCAGATTATTAAAGTAGTAATATTACTTGCACAGTTCGAAAATGAAT
TTAATGCCCCAATTCTTGCATGCGTAAGAGGCGATCAACACATTAATGAAGTAAAGCTTTTTAACTTAATCAATAAACTT
CATCACTTCAATCTCCTTAATCTTAAAAAGATTGAAGACAAAAATACTATCGAAAAAAATCTAGTTGATTTACCCTTAGG
TTTTATTGGGCCAGATTTAGATAATAAAACTATTAAAGCTAGTTCTAATTGGGAGAAAAAATGGACAAGAATAATTGACC
ATTCTGCAAGTGACCTTTCAAAGTTTATAAGTGGTGGGAATAAAGTTAATTTCCATAAAGTTTTTCAAGAATTTTCTTTT
AATTCAAAAAACTATCTAATTGGGGATATAAGAAATGCAAAAAAAGGAGATAAAATAAGCATTGATGATGATGAAGAACT
TAAAGAAAAAAAGGGTATCGAGATTGGACATATTTTCCAACTAGGTCAGAAATATAGTGAAAAATTAAATGCAAAGTTCT
CTGATAAGGACGGTCAGTTAAAAAATTTATGGATGGGTTGTTACGGAATAGGAGTGACAAGAATAGCTCAAGCTGCTATC
GAACAGAATCATGATCAAAAAGGAATTTGCTGGCCTATCCAGATTTCTCCTTTTGAAGTGATTATTATCCCAACAAACCT
AAAAGATCCTATTCAAAGTGATCTTACTGAGCAAATCTACAACAACTTTTTAATTAATAAAATTGATGTCCTTCTAGATG
ATAGAAACGATAGGGCTGGAGTGAAATTTAAAGATGCGGAATTAATTGGTATTCCTTTTCAGGTAATTATTGGCAGAGAT
TCTATTAATAAAGAAGTAGAACTTTTTTGCAGAACAAATAATACTAAGATTAAAATTAGTACTGATAAATTGTTAGAAAC
ATTTATTTCCGAATCTGAAATAATGTACAATAAAAAGTCTTGA

Upstream 100 bases:

>100_bases
AAAGAATATTCTTAGACTAGCCGTGTTGAGGGACTCAAGACACTTTAAATTATTTTTTCACCTTAATTGATAAAATTAAT
TTTATTAAGGATTTTTCTGT

Downstream 100 bases:

>100_bases
GGCTTATTTTTTTTGGGAGCAAAGTTATGTTACTGAAATGGACATCAAAATTATTTTTTAAGAATCTTTCCAAAGCTATA
TCTTTTGCAATATCCTTAAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVVEKISAIIEKELNSIGCTKLLLPQLHPADL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSENDLASFYEKVGNAYENIFESCGLQTVGVDADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSLPSQPIPLKDNIAEWLETPNQKTIQEVCDNNNLDPSQIIKVVILLAQFENEFNAPILACVRGDQHINEVKLFNLINKL
HHFNLLNLKKIEDKNTIEKNLVDLPLGFIGPDLDNKTIKASSNWEKKWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSF
NSKNYLIGDIRNAKKGDKISIDDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIQISPFEVIIIPTNLKDPIQSDLTEQIYNNFLINKIDVLLDDRNDRAGVKFKDAELIGIPFQVIIGRD
SINKEVELFCRTNNTKIKISTDKLLETFISESEIMYNKKS

Sequences:

>Translated_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVVEKISAIIEKELNSIGCTKLLLPQLHPADL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSENDLASFYEKVGNAYENIFESCGLQTVGVDADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSLPSQPIPLKDNIAEWLETPNQKTIQEVCDNNNLDPSQIIKVVILLAQFENEFNAPILACVRGDQHINEVKLFNLINKL
HHFNLLNLKKIEDKNTIEKNLVDLPLGFIGPDLDNKTIKASSNWEKKWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSF
NSKNYLIGDIRNAKKGDKISIDDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIQISPFEVIIIPTNLKDPIQSDLTEQIYNNFLINKIDVLLDDRNDRAGVKFKDAELIGIPFQVIIGRD
SINKEVELFCRTNNTKIKISTDKLLETFISESEIMYNKKS
>Mature_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVVEKISAIIEKELNSIGCTKLLLPQLHPADL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSENDLASFYEKVGNAYENIFESCGLQTVGVDADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSLPSQPIPLKDNIAEWLETPNQKTIQEVCDNNNLDPSQIIKVVILLAQFENEFNAPILACVRGDQHINEVKLFNLINKL
HHFNLLNLKKIEDKNTIEKNLVDLPLGFIGPDLDNKTIKASSNWEKKWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSF
NSKNYLIGDIRNAKKGDKISIDDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIQISPFEVIIIPTNLKDPIQSDLTEQIYNNFLINKIDVLLDDRNDRAGVKFKDAELIGIPFQVIIGRD
SINKEVELFCRTNNTKIKISTDKLLETFISESEIMYNKKS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=43.6681222707424, Blast_Score=193, Evalue=5e-49,
Organism=Escherichia coli, GI1786392, Length=591, Percent_Identity=41.4551607445008, Blast_Score=450, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI115532348, Length=213, Percent_Identity=36.150234741784, Blast_Score=133, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320931, Length=225, Percent_Identity=36.4444444444444, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24656200, Length=226, Percent_Identity=38.0530973451327, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PROM0 (A3PBN3)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090759.1
- ProteinModelPortal:   A3PBN3
- SMR:   A3PBN3
- STRING:   A3PBN3
- GeneID:   4912249
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_05351
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 67939; Mature: 67939

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVVEKISAIIEK
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELNSIGCTKLLLPQLHPADLWKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITS
HHHHCCCHHHHCCCCCHHHHHHCHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSENDLASFYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VGNAYENIFESCGLQTVGVDADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHH
VSLPSQPIPLKDNIAEWLETPNQKTIQEVCDNNNLDPSQIIKVVILLAQFENEFNAPILA
HCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
CVRGDQHINEVKLFNLINKLHHFNLLNLKKIEDKNTIEKNLVDLPLGFIGPDLDNKTIKA
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCEEEC
SSNWEKKWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSFNSKNYLIGDIRNAKKGDKIS
CCCHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCEEC
IDDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDQKGICWPIQISPFEVIIIPTNLKDPIQSDLTEQIYNNFLINKIDVLLDDRNDRAG
HCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
VKFKDAELIGIPFQVIIGRDSINKEVELFCRTNNTKIKISTDKLLETFISESEIMYNKKS
CEEECCEEECCCEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHCCCHHEECCCC
>Mature Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVVEKISAIIEK
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELNSIGCTKLLLPQLHPADLWKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITS
HHHHCCCHHHHCCCCCHHHHHHCHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSENDLASFYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VGNAYENIFESCGLQTVGVDADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHH
VSLPSQPIPLKDNIAEWLETPNQKTIQEVCDNNNLDPSQIIKVVILLAQFENEFNAPILA
HCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
CVRGDQHINEVKLFNLINKLHHFNLLNLKKIEDKNTIEKNLVDLPLGFIGPDLDNKTIKA
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCEEEC
SSNWEKKWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSFNSKNYLIGDIRNAKKGDKIS
CCCHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCEEC
IDDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDQKGICWPIQISPFEVIIIPTNLKDPIQSDLTEQIYNNFLINKIDVLLDDRNDRAG
HCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
VKFKDAELIGIPFQVIIGRDSINKEVELFCRTNNTKIKISTDKLLETFISESEIMYNKKS
CEEECCEEECCCEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHCCCHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA