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The map label for this gene is ytsJ [H]

Identifier: 126698589

GI number: 126698589

Start: 1173616

End: 1174800

Strand: Direct

Name: ytsJ [H]

Synonym: CD1005

Alternate gene names: 126698589

Gene position: 1173616-1174800 (Clockwise)

Preceding gene: 126698588

Following gene: 126698590

Centisome position: 27.36

GC content: 32.07

Gene sequence:

>1185_bases
TTGATGTCTAAGGATTATGCAAAATTGGCATTAGAAATGCATGAAATCAACAAAGGGAAAGTAAGTGTAGAAAGCAAGGT
AGAAATTAAAACTAAGGATGATTTATCAACAGCATATACACCAGGGGTTGCTGAACCTTGTTTAAAAATACATGAAAATC
AAGATGATGTTTATAGATACACTTCTAAAGGGAATCTAGTAGCAGTAGTTAGTGATGGAAGTGCTGTACTTGGATTAGGT
AACATTGGTGCTGAAGCATCTATACCTGTTATGGAAGGTAAAGCTATATTATTTAAACAATTTGCAGATGTAGACGCTTT
TCCAATATGTTTAAAGACTAATGATGTAGATGAGATAGTTAAATCTGTAGAGTTGATGGAGCCTGTATTTGGAGGTATAA
ATTTGGAAGACATATCTTCTCCAAGATGTTTTGAGATAGAAGAGAGATTAAAAAAATCTTTAAGTATACCTGTTTTTCAT
GATGACCAACATGGGACAGCAATAATAGTTGCAGCAGCCATAATAAACTCAATAAAGTTAATTGAAAATAAAAAAATAGA
AGATTTAGAAATAGTTATAAACGGAGCAGGAGCTGCTGGTATAGCCATAGCAAAGATACTACTTAATATGAATGTTAAAA
ATATAATTTTATGTGATAGAAGTGGAGCATTGGAAGCTAGTATAGAAAATCTAAACTATGTTCAAAAAGAAATGTTAAAA
GTAACAAATATAAGAAATGAAAAAGGACCTTTAAAAGATATTATAAAAGGAAAAGATGTATTTATTGGTGTTTCAGGTCC
AGGAGCTGTTACAAAGGAAATGGTTGAAAGTATGTCTGAGAAGCCAATAATATTGGCAATGGCAAATCCTACTCCAGAGA
TAATGCCAGAAGAAGCAAAATTAGGTGGAGCATTTATAATGGGAACAGGTCGTTCAGACTTCCCTAATCAAGTCAATAAT
GTACTGGCTTTTCCTGGAATATTTAGAGGTGCACTTGATGTTAAGGCTAAAGAGATAAATGAAGAAATGAAGATAGCTGC
TGCATATGCAATTGCTAATACAATAAGTGATGAAGAAATATGCCCAGAGTATATATTACCAGATGTATTTAATAAGAATG
TAGTAAAAAATGTTGCTAAGGCAGTTAAAGAAGCAGCGATAAAAACCAAAGTAAATAGAATATAA

Upstream 100 bases:

>100_bases
TATATGAGCAAGGTCGAGCAATTTATGAATGTAAATAATGTTTAGAAATAAATATGAATATCAAAATTAAAAATAGTTGT
GTATATTTTAAGGAGGAAAA

Downstream 100 bases:

>100_bases
ATTTTTATATGTTCATATATCCTTATCTTATCGGGTATATGGGCATTTTTATTATAGGTTTATACTCATATATCCTTACT
TTTATATACATATCTTTATG

Product: NAD-dependent malic enzyme

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MMSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAEPCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLG
NIGAEASIPVMEGKAILFKQFADVDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFH
DDQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCDRSGALEASIENLNYVQKEMLK
VTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVESMSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNN
VLAFPGIFRGALDVKAKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNRI

Sequences:

>Translated_394_residues
MMSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAEPCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLG
NIGAEASIPVMEGKAILFKQFADVDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFH
DDQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCDRSGALEASIENLNYVQKEMLK
VTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVESMSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNN
VLAFPGIFRGALDVKAKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNRI
>Mature_394_residues
MMSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAEPCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLG
NIGAEASIPVMEGKAILFKQFADVDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFH
DDQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCDRSGALEASIENLNYVQKEMLK
VTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVESMSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNN
VLAFPGIFRGALDVKAKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNRI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=387, Percent_Identity=24.8062015503876, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI62420882, Length=383, Percent_Identity=24.8041775456919, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI62420880, Length=383, Percent_Identity=24.8041775456919, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI239049447, Length=383, Percent_Identity=24.8041775456919, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4505145, Length=389, Percent_Identity=25.4498714652956, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788806, Length=408, Percent_Identity=47.7941176470588, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI87081919, Length=399, Percent_Identity=25.31328320802, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=348, Percent_Identity=27.0114942528736, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281363503, Length=417, Percent_Identity=26.6187050359712, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281363505, Length=417, Percent_Identity=26.6187050359712, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI78707236, Length=417, Percent_Identity=26.6187050359712, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI78707232, Length=410, Percent_Identity=27.0731707317073, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI78707242, Length=369, Percent_Identity=28.4552845528455, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI78707238, Length=369, Percent_Identity=28.4552845528455, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI78707240, Length=367, Percent_Identity=28.6103542234332, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281362672, Length=434, Percent_Identity=26.036866359447, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356279, Length=434, Percent_Identity=26.036866359447, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281362674, Length=434, Percent_Identity=26.036866359447, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42713; Mature: 42713

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAEPCLKIHENQDDVYRY
CCCCHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCHHCCCCHHHHHHHHCCCCCCEEEE
TSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFADVDAFPICLKTNDVDEIV
ECCCCEEEEEECCCEEEEECCCCCCCCCEEECCCHHHHHHHCCCCCEEEEEECCCHHHHH
KSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHDDQHGTAIIVAAAIINSIKL
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHH
IENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCDRSGALEASIENLNYVQKEMLK
HCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVESMSEKPIILAMANPTPEIMPEEAK
HHHCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
LGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVKAKEINEEMKIAAAYAIANTISDEEI
CCCEEEEECCCCCCCHHHHHEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHC
CPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNRI
CHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAEPCLKIHENQDDVYRY
CCCCHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCHHCCCCHHHHHHHHCCCCCCEEEE
TSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFADVDAFPICLKTNDVDEIV
ECCCCEEEEEECCCEEEEECCCCCCCCCEEECCCHHHHHHHCCCCCEEEEEECCCHHHHH
KSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHDDQHGTAIIVAAAIINSIKL
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHH
IENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCDRSGALEASIENLNYVQKEMLK
HCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVESMSEKPIILAMANPTPEIMPEEAK
HHHCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
LGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVKAKEINEEMKIAAAYAIANTISDEEI
CCCEEEEECCCCCCCHHHHHEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHC
CPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNRI
CHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]