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The map label for this gene is leuC

Identifier: 126698574

GI number: 126698574

Start: 1154680

End: 1155957

Strand: Direct

Name: leuC

Synonym: CD0990

Alternate gene names: 126698574

Gene position: 1154680-1155957 (Clockwise)

Preceding gene: 126698573

Following gene: 126698575

Centisome position: 26.91

GC content: 34.19

Gene sequence:

>1278_bases
ATGGGGATGACTATGACGCAGAAAATATTAGCCAAACATGCTAAATTAAATGAAGTTAAGAAAGGGCAATTAATCGAAGC
AGATTTAGATGTAGTTTTAGGTAATGATATAACATCACCAGTAGCCATTAGAGAATTTGAAAAATTAGGAATTGAAGATG
TTTATGATAAGACTAAAGTAGTGATGGTTTTAGACCACTTTACACCTAATAAAGATATAAAGAGTGCAGAACAATGTAAA
TTTACAAGAAGTTTCGCAAAATCAAAAGGAGTTGTAAATTTTTTTGATGTAGGAGATATGGGAATAGAGCATGTTCTTCT
TCCTGAAAAAGGTATAGTAACTGCTGGAGATGTAATTATAGGTGCTGACTCTCATACTTGCACATATGGAGCATTAGGAG
CATTTTCTACTGGTGTAGGTTCAACTGATATGGGAGCAGGTATGGCTACAGGAAAATGTTGGTTTAAGGTTCCTGGAGCA
ATAAAATTTGTTTTGAAAAATAAACCAAATAAATGGATAAGTGGTAAAGATATAATACTTCATATAATAGGGGAGATAGG
TGTTGATGGAGCACTTTACAAGTCAATGGAATTTTGTGGAGATGGAGTAGAATACTTATCTATGGATGATAGATTTACTA
TTTGTAATATGGCAATAGAAGCAGGAGCAAAAAATGGAATTTTTCCAGTAGACGATAAGACTATGGAATATATAAATTCA
CATAAGTGTTCAACTATGACAAAAGATGTAAATATATATGAAGCAGATGAAGATGCAGTCTATGATGAAGTATACGAAAT
AGATTTAGCCAAACTAAAAGAAACAGTAGCCTTTCCTCATCTTCCTGAAAATACTAGAACAGTAGATGAGATAGATAAGG
ATATAAAGATAGACCAAGTTGTAATAGGCTCTTGTACAAATGGAAGAATATCAGACCTTGAAGTAGTAGCTGAGATAATG
AAAGGCAAAAAAGTAGCAGATGGAGTTAGAGTTATGATATTTCCTGGAACTCAAAAAGTATATCTTGAAGCTATAGAAAA
AGGGTATATTACCACTTTTATAGAGGCAGGAGCAGCAGTTTCTACACCAACTTGTGGTCCATGTCTAGGGGGACATATGG
GTATACTTGCAGCAGGAGAAAAATCCATTTCAACAACAAATAGGAATTTCGTAGGAAGAATGGGGCATGTTGATTCAGAG
GTGTATCTTGCAAGCCCAGCTGTAGCAGCAGCATCAGCTATAACTGGAAAAATATCAAAACCATCAGAAATAATTTAA

Upstream 100 bases:

>100_bases
GTCTATTTTGGTGTAAGTATATCGGAAAACATAAATACAGCAGCTATTAATGCACTTATGAATGCAATAAATAAAAGTTA
TATTGAGGAGGAAATAAAAA

Downstream 100 bases:

>100_bases
GGGAGGTAAAATTTATGATTGCCAATGGAAGTGTTTTTAAATTCGGTGACAATATAGATACAGATGTAATAATACCAGCT
AGATATTTAAATATAGCTGA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIREFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCK
FTRSFAKSKGVVNFFDVGDMGIEHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMAIEAGAKNGIFPVDDKTMEYINS
HKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETVAFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIM
KGKKVADGVRVMIFPGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVGRMGHVDSE
VYLASPAVAAASAITGKISKPSEII

Sequences:

>Translated_425_residues
MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIREFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCK
FTRSFAKSKGVVNFFDVGDMGIEHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMAIEAGAKNGIFPVDDKTMEYINS
HKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETVAFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIM
KGKKVADGVRVMIFPGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVGRMGHVDSE
VYLASPAVAAASAITGKISKPSEII
>Mature_424_residues
GMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIREFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKF
TRSFAKSKGVVNFFDVGDMGIEHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGAI
KFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMAIEAGAKNGIFPVDDKTMEYINSH
KCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETVAFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMK
GKKVADGVRVMIFPGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVGRMGHVDSEV
YLASPAVAAASAITGKISKPSEII

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=450, Percent_Identity=27.5555555555556, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI8659555, Length=370, Percent_Identity=26.2162162162162, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=25, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1786259, Length=469, Percent_Identity=31.9829424307036, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI87081781, Length=349, Percent_Identity=25.7879656160458, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.7617728531856, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367097, Length=458, Percent_Identity=25.1091703056769, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=450, Percent_Identity=29.1111111111111, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=352, Percent_Identity=30.6818181818182, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=285, Percent_Identity=29.8245614035088, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=450, Percent_Identity=24.2222222222222, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=35.2549889135255, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=431, Percent_Identity=32.2505800464037, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323335, Length=453, Percent_Identity=31.5673289183223, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322261, Length=460, Percent_Identity=28.4782608695652, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI28571643, Length=446, Percent_Identity=28.0269058295964, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=27.0089285714286, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=27.0089285714286, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161076999, Length=347, Percent_Identity=29.1066282420749, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645686, Length=372, Percent_Identity=25.2688172043011, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=26.0162601626016, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_CLOD6 (Q18AJ2)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087471.1
- ProteinModelPortal:   Q18AJ2
- SMR:   Q18AJ2
- STRING:   Q18AJ2
- GeneID:   4914340
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0990
- NMPDR:   fig|1496.1.peg.3889
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- ProtClustDB:   CLSK2534650
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45849; Mature: 45718

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIREFEKLGIEDVYDKTKV
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCHHHHHHHHCCCHHHHCCCEE
VMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGIEHVLLPEKGIVTAGDVII
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCEEECCEEE
GADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGAIKFVLKNKPNKWISGKDIIL
ECCCCCEECCCCHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEEE
HIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMAIEAGAKNGIFPVDDKTMEYINS
EEEHHHCCCHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHC
HKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETVAFPHLPENTRTVDEIDKDIKIDQV
CCCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEECEE
VIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIFPGTQKVYLEAIEKGYITTFIEAGAAV
EEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEHHCCCCC
STPTCGPCLGGHMGILAAGEKSISTTNRNFVGRMGHVDSEVYLASPAVAAASAITGKISK
CCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHHHHHCCCCC
PSEII
CCCCC
>Mature Secondary Structure 
GMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIREFEKLGIEDVYDKTKV
CCCHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCHHHHHHHHCCCHHHHCCCEE
VMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGIEHVLLPEKGIVTAGDVII
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCEEECCEEE
GADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGAIKFVLKNKPNKWISGKDIIL
ECCCCCEECCCCHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEEE
HIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMAIEAGAKNGIFPVDDKTMEYINS
EEEHHHCCCHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHC
HKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETVAFPHLPENTRTVDEIDKDIKIDQV
CCCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEECEE
VIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIFPGTQKVYLEAIEKGYITTFIEAGAAV
EEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEHHCCCCC
STPTCGPCLGGHMGILAAGEKSISTTNRNFVGRMGHVDSEVYLASPAVAAASAITGKISK
CCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHHHHHCCCCC
PSEII
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA