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The map label for this gene is leuD

Identifier: 126698575

GI number: 126698575

Start: 1155972

End: 1156463

Strand: Direct

Name: leuD

Synonym: CD0991

Alternate gene names: 126698575

Gene position: 1155972-1156463 (Clockwise)

Preceding gene: 126698574

Following gene: 126698576

Centisome position: 26.94

GC content: 29.88

Gene sequence:

>492_bases
ATGATTGCCAATGGAAGTGTTTTTAAATTCGGTGACAATATAGATACAGATGTAATAATACCAGCTAGATATTTAAATAT
AGCTGATTATAAAGAACTAGCAACGCATTGTATGGAGGATATTGATGATAAATTTATCTCTAAGGTAAAAAAAGGAGATA
TTATAGTTGCAACTAAAAACTTTGGTTGTGGTTCATCAAGAGAACATGCACCAATTGTAATAAAAGAAAGTGGAGTATCC
TGTGTGATTGCTTCTACTTTTGCTAGAATATTTTTTAGAAACTCTATAAATATAGGGCTTCCTATACTTGAATGTGAAGA
AGCTGCCAATAACATAGATGAAGGGGATAATATAGAAGTAGATTTTTCTACAGGTGTGATAAAAAATATAACAAAAGGAA
AAGAATATAAAGCAGAACCATTTCCTGAGTTTATGCAAAATATTATATTAAATGAAGGCTTAATTAACTCAATAAAAGCA
AATAGGGGGTAG

Upstream 100 bases:

>100_bases
ATTCAGAGGTGTATCTTGCAAGCCCAGCTGTAGCAGCAGCATCAGCTATAACTGGAAAAATATCAAAACCATCAGAAATA
ATTTAAGGGAGGTAAAATTT

Downstream 100 bases:

>100_bases
TGGAGTATGAATTGTAATATAGCAGTCATAAAAGGTGATGGTGTTGGACCGGAAATTATAGATGAAGGTATAAAGGTTCT
AAATAAAATTTGTTGTAAAT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDKFISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVS
CVIASTFARIFFRNSINIGLPILECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
NRG

Sequences:

>Translated_163_residues
MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDKFISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVS
CVIASTFARIFFRNSINIGLPILECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
NRG
>Mature_163_residues
MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDKFISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVS
CVIASTFARIFFRNSINIGLPILECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
NRG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=109, Percent_Identity=36.697247706422, Blast_Score=70, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=96, Percent_Identity=44.7916666666667, Blast_Score=81, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=34.9514563106796, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_CLOD6 (Q18AJ1)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087472.1
- ProteinModelPortal:   Q18AJ1
- SMR:   Q18AJ1
- STRING:   Q18AJ1
- GeneID:   4914341
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0991
- NMPDR:   fig|1496.1.peg.3888
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- ProtClustDB:   CLSK2534651
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02084
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17965; Mature: 17965

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDKFISKVKKGDIIVATKN
CCCCCCEEEECCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
FGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPILECEEAANNIDEGDNIEV
CCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCCCCEEE
DFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKANRG
EECCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure
MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDKFISKVKKGDIIVATKN
CCCCCCEEEECCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
FGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPILECEEAANNIDEGDNIEV
CCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCCCCEEE
DFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKANRG
EECCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA