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The map label for this gene is leuA [H]

Identifier: 126698573

GI number: 126698573

Start: 1152981

End: 1154723

Strand: Direct

Name: leuA [H]

Synonym: CD0989

Alternate gene names: 126698573

Gene position: 1152981-1154723 (Clockwise)

Preceding gene: 126698572

Following gene: 126698574

Centisome position: 26.87

GC content: 31.33

Gene sequence:

>1743_bases
ATGAAGTGTGGAAAGTATAAAAAATATGATAAAATGCAAATAGTAAATCGTAAATGGCCAGATAATGAAATATTTAAGGC
TCCAATTTGGTGTAGTGTCGATTTAAGAGACGGAAATCAGTCACTGCCAACACCTATGAGTGTAAATGAAAAAGTCAGAA
TGTTCAAAATGCTTATTGATACAGGATTTAAAGAAATAGAGGTAGGATTTCCATCAGCATCAAATACAGAGTATACATTT
TTAAGAAAATTAATAGATGAGAATATGATTCCAGATGATGTAACTATACAAGTACTTACACAATCTAGGGCTCATCTTAT
AGAAAAAACATTTGAATCAATAAGGGGATGTAAAAAAGCAATTATACATTTATATAACTCTACATCTGTTCTTCAAAGAG
ATGTGGTTTTTAATATGAGTAAACAAGAAATAATAGATATAGCAGTTGAAGGAGCAAAATTATTTAATGAAGAAGTAAAA
AAATATCCAGAAACAGAATTTACTTTTGAATATTCACCAGAGAGTTTTACAGGAACAGAGATGGATTATGCACTTGAAAT
ATGTGAGGCAGTGATAGATGTTTGGAAACCAACTCCTCAAAAAAAAGTAATAATAAATCTTCCATCAACAGTTGAAATGG
CAACACCAAACGTATATGCAGACCAAATAGAATGGTTTTGTAAAAATATAAGTTGTAGGGATTCTATTATACTAAGTTTG
CATACTCACAATGATAGAGGAACTTGTACGGCTGCAAGTGAACTTGGATTATTGGCTGGTGCCGATAGACTTGAAGGAAC
ACTATTTGGAAATGGTGAAAGAACTGGGAATATGGATATAGTAAATGTGGGACTTAATCTTTATACACAAGGAATAGACC
CAGAGCTAGATTTTAGCAATATAGATAAGATAATAGGTATATATGAAGACTGTACAAAACTTATGGTACATGATAGACAT
CCATATGCAGGTAATCTTGTACATTGTGCTTTTTCAGGTTCACATCAAGATGCTATTAGAAAAGGCATGATAGCAATGAA
AAACAGAGATAATGATTACTGGGAAGTTCCATATTTACCAATAGACCCTCATGATATTGGAAGAGAATACAAAGAAATTA
TAAGGATAAATAGCCAATCTGGAAAAGGAGGAGCTGTTTATATAATGGAAACAGATTATGGATTTATGATACCTAAGAAT
ATGCATTCTGATTTTGGAAATGTAGTCAAAATGGAATCTGATAGAATTGGGGAAGAACTTAGTAGTGAAGCAATATTTAA
TTTGTTTAAGAAAGAATATATTGAAGTTGAGAGCCCTTATAAAGTTAAGAAGTATAAAATAAAATCAATGGATGAATTAA
ACTATGAAAATGATGATTCTAATGATACTAACATGATTGAGATGACTGCAAGAATAAGTTATATGGGAAATGAACAAAGG
ATTGTTGGTATAGGAAATGGACCTGTAGATTCATTTAATAATGCACTAAAGCAATGTGGCATGAAAGATTATAAATTTAG
ATATTACTGGGAACATGCATTAGAGGAAGGGTCACATTCAAGAGGGGTAGCTTATGTAGGAATAGAGCACAATAATGAAG
TCTATTTTGGTGTAAGTATATCGGAAAACATAAATACAGCAGCTATTAATGCACTTATGAATGCAATAAATAAAAGTTAT
ATTGAGGAGGAAATAAAAAATGGGGATGACTATGACGCAGAAAATATTAGCCAAACATGCTAA

Upstream 100 bases:

>100_bases
TATTCTAATGATAAAATTGACATTTATCCTTATGAATTGTGCAGGATTGCGTCGATATGAGGTATACTGAATACAAAAAA
CAGAAAAAGGGGGAAATAAC

Downstream 100 bases:

>100_bases
ATTAAATGAAGTTAAGAAAGGGCAATTAATCGAAGCAGATTTAGATGTAGTTTTAGGTAATGATATAACATCACCAGTAG
CCATTAGAGAATTTGAAAAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPTPMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTF
LRKLIDENMIPDDVTIQVLTQSRAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNVYADQIEWFCKNISCRDSIILSL
HTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTGNMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRH
PYAGNLVHCAFSGSHQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETDYGFMIPKN
MHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSMDELNYENDDSNDTNMIEMTARISYMGNEQR
IVGIGNGPVDSFNNALKQCGMKDYKFRYYWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSY
IEEEIKNGDDYDAENISQTC

Sequences:

>Translated_580_residues
MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPTPMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTF
LRKLIDENMIPDDVTIQVLTQSRAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNVYADQIEWFCKNISCRDSIILSL
HTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTGNMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRH
PYAGNLVHCAFSGSHQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETDYGFMIPKN
MHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSMDELNYENDDSNDTNMIEMTARISYMGNEQR
IVGIGNGPVDSFNNALKQCGMKDYKFRYYWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSY
IEEEIKNGDDYDAENISQTC
>Mature_580_residues
MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPTPMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTF
LRKLIDENMIPDDVTIQVLTQSRAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNVYADQIEWFCKNISCRDSIILSL
HTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTGNMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRH
PYAGNLVHCAFSGSHQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETDYGFMIPKN
MHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSMDELNYENDDSNDTNMIEMTARISYMGNEQR
IVGIGNGPVDSFNNALKQCGMKDYKFRYYWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSY
IEEEIKNGDDYDAENISQTC

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=539, Percent_Identity=21.8923933209648, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324225, Length=565, Percent_Identity=46.5486725663717, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6324682, Length=567, Percent_Identity=45.5026455026455, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 66157; Mature: 66157

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPTPMSVNEKVRMFKMLID
CCCCCCCCCCHHHHHCCCCCCCCEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
TGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQSRAHLIEKTFESIRGCKKA
CCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
IIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVKKYPETEFTFEYSPESFTGTE
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
MDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNVYADQIEWFCKNISCRDSIILSL
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEE
HTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTGNMDIVNVGLNLYTQGIDPELDFSN
EECCCCCCEEEHHHCCHHCCCHHCCEEEEECCCCCCCEEEEEECEEEEECCCCCCCCHHH
IDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGSHQDAIRKGMIAMKNRDNDYWEVPYLP
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHCCEEEECCCCCEEECCEEC
IDPHDIGREYKEIIRINSQSGKGGAVYIMETDYGFMIPKNMHSDFGNVVKMESDRIGEEL
CCHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCEECCCHHCCCCCCEEEECHHHHHHHH
SSEAIFNLFKKEYIEVESPYKVKKYKIKSMDELNYENDDSNDTNMIEMTARISYMGNEQR
HHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCCCCEEEEEEEEEEECCCCCE
IVGIGNGPVDSFNNALKQCGMKDYKFRYYWEHALEEGSHSRGVAYVGIEHNNEVYFGVSI
EEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEEE
SENINTAAINALMNAINKSYIEEEIKNGDDYDAENISQTC
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure
MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPTPMSVNEKVRMFKMLID
CCCCCCCCCCHHHHHCCCCCCCCEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
TGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQSRAHLIEKTFESIRGCKKA
CCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
IIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVKKYPETEFTFEYSPESFTGTE
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
MDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNVYADQIEWFCKNISCRDSIILSL
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEE
HTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTGNMDIVNVGLNLYTQGIDPELDFSN
EECCCCCCEEEHHHCCHHCCCHHCCEEEEECCCCCCCEEEEEECEEEEECCCCCCCCHHH
IDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGSHQDAIRKGMIAMKNRDNDYWEVPYLP
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHCCEEEECCCCCEEECCEEC
IDPHDIGREYKEIIRINSQSGKGGAVYIMETDYGFMIPKNMHSDFGNVVKMESDRIGEEL
CCHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCEECCCHHCCCCCCEEEECHHHHHHHH
SSEAIFNLFKKEYIEVESPYKVKKYKIKSMDELNYENDDSNDTNMIEMTARISYMGNEQR
HHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCCCCEEEEEEEEEEECCCCCE
IVGIGNGPVDSFNNALKQCGMKDYKFRYYWEHALEEGSHSRGVAYVGIEHNNEVYFGVSI
EEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEEE
SENINTAAINALMNAINKSYIEEEIKNGDDYDAENISQTC
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]