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The map label for this gene is purU-2 [H]

Identifier: 126447488

GI number: 126447488

Start: 1891903

End: 1892778

Strand: Direct

Name: purU-2 [H]

Synonym: BMA10247_A1968

Alternate gene names: 126447488

Gene position: 1891903-1892778 (Clockwise)

Preceding gene: 126446644

Following gene: 126447408

Centisome position: 80.41

GC content: 66.21

Gene sequence:

>876_bases
ATGTCCGTTCCACAACGTCCCCATCAGTTCGTGCTGACGCTGTCATGTCCGAGCGCCGCGGGCCAGGTCGCCGCCGTCGT
CGGCCTGCTCGACCGGCATCGCTGCTACGTCGACGAGCTGACCGTCTTCGACGACGATCTCAGCGCGCGCTTTTTCGTGC
GTTGCGTGTTCCATGCGACCGACGACGCCGACGCGCTGCGCGTCGACGCGCTGCGACGCGAGTTCGAACCGATCGCCGAG
CGCTTCCGGATGCAATGGGCGATTCACGACGTCGCCGCGCGGCCGAAGGTGCTGATCATGGTGTCGAAGCTCGAGCATTG
CCTCGCCGATCTGCTGTTCCGCTGGAAGATGGGCGAGCTGAAGATGGACATCGTCGGGATCGTGTCGAATCATCCGGACT
TCGCGCCGCTCGCCGCGCAGCACGGGCTGCCGTTCCGGCACTTCCCGATCACGGCCGACACGAAGGCGCAGCAGGAGGCC
CAATGGCTCGACGTGTTCGAGACGAGCGGCGCCGAGCTCGTGATCCTCGCGCGCTACATGCAGGTGCTGTCGCCGGAAGC
GAGCGCGAGGCTCGCGAATCGCGCGATCAACATCCATCACTCGTTCCTGCCGGGGTTCAAGGGCGCGAAGCCCTACCATC
AGGCGCATGCTCGCGGCGTGAAGCTGATCGGCGCGACCGCGCACTTCGTGACCGACGACCTCGACGAGGGGCCGATCATC
GAGCAGGTGGTCGAGCGCGTCGATCACTCGTACCGGCCCGAACAGTTGCTCGCGGTGGGGCGCGACGTCGAATGCATCAC
GCTCGCGCGCGCCGTGAAGGCATTCATCGAGCGGCGGGTGTTCCTGAATGGCGACCGGACCGTCGTGTTCCAGTAG

Upstream 100 bases:

>100_bases
GGTTCTATCCGCATCCGATCTGGCTTGATTAACTGGCGTATCGCCTCGGACGCCCGGCATCCGCGCCGCGCGCGGGGCGC
CATCGAGTCAGAGGAAACCC

Downstream 100 bases:

>100_bases
CGGGCGCGCCGACCGCCGCGGCCTCGGCATGTTGCCTGCGCCCGGATGCGGCGACGCATCCGGGCATCCTCGCCGGCCCA
CGCCGGCTATCGCAGCCAGG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSVPQRPHQFVLTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDLSARFFVRCVFHATDDADALRVDALRREFEPIAE
RFRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGELKMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEA
QWLDVFETSGAELVILARYMQVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
EQVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFQ

Sequences:

>Translated_291_residues
MSVPQRPHQFVLTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDLSARFFVRCVFHATDDADALRVDALRREFEPIAE
RFRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGELKMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEA
QWLDVFETSGAELVILARYMQVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
EQVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFQ
>Mature_290_residues
SVPQRPHQFVLTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDLSARFFVRCVFHATDDADALRVDALRREFEPIAER
FRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGELKMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEAQ
WLDVFETSGAELVILARYMQVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFQ

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1787483, Length=287, Percent_Identity=37.9790940766551, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788846, Length=150, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=160, Percent_Identity=38.75, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32794; Mature: 32663

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPQRPHQFVLTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDLSARFFVRCVFHAT
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHEEEEECC
DDADALRVDALRREFEPIAERFRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCE
KMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEAQWLDVFETSGAELVILARYM
EEEEEEEECCCCCCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
QVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
HHHCCCHHHHHHHHHEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCCCHH
EQVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFQ
HHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCEEEEEC
>Mature Secondary Structure 
SVPQRPHQFVLTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDLSARFFVRCVFHAT
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHEEEEECC
DDADALRVDALRREFEPIAERFRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCE
KMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEAQWLDVFETSGAELVILARYM
EEEEEEEECCCCCCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
QVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
HHHCCCHHHHHHHHHEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCCCHH
EQVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFQ
HHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]