Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 126446811

GI number: 126446811

Start: 2097846

End: 2099141

Strand: Direct

Name: mdeA [H]

Synonym: BMA10247_A2164

Alternate gene names: 126446811

Gene position: 2097846-2099141 (Clockwise)

Preceding gene: 126446279

Following gene: 126446240

Centisome position: 89.17

GC content: 67.36

Gene sequence:

>1296_bases
ATGACCGAACCCGCATCCGCCGACTGGCGCCTCGAGACCCTCGCCGTGCACGGCGGCTATCGCCCCGATCCGACGACGCG
CGCCGCCGCGGTGCCGATCTATCAGACGGTCGCGTTCGCGTTCGACGACACGCAGCACGGCGCGGACCTGTTCGATCTGA
AGGTCCAGGGCAACATCTATTCGCGGATCATGAATCCGACCAACGACGTGCTCGAGCAGCGCGTCGCGGCGCTCGAAGGC
GGCATCGGCGCGCTCGCGCTCGCATCGGGCCAGGCGGCCGTCACTTATTCGATCCTGACGATCGCGGAGGCGGGCGACAA
CATCGTATCGTCGAGCACGCTGTACGGCGGCACCTACAACCTGTTCGCGCATACGCTGCCGCAATACGGAATCTCGACGC
GCTTCGCCGACCCGCGCGAGCCGGCCGCGTTCGACGCGCTGATCGACGCACGGACGAAGGCGATCTTCGCGGAATCGGTC
GGCAATCCGCTCGGCAACATCACCGATATCGCCGCGCTCGCCGAGATCGCGCACCGCCACGGCCTGCCGCTCATCGTCGA
CAACACGGTGCCGACGCCCTACCTGCTGCGCCCGTTCGAGCATGGCGCGGATATCGTCGTCCATTCGCTGACGAAGTATC
TCGGCGGGCACGGCACGAGCCTCGGCGGCGCGATCGTCGATTCGGGCAAGTTTCCGTGGGCGAAGCACGCCGAGCGCTTC
CGGCGGCTGAACGAGCCGGACGTCAGCTATCACGGCGTCGTCTACACCGACGCGTTCGGCGCAGCGGCGTACATCGGCCG
CGCGCGCGTCGTTCCGCTGCGCAACACGGGCGCGGCGCTTTCGCCGTTCAACGCATTCCAGATCCTGCAAGGCATCGAGA
CGCTCGCGCTGCGCATCGAGCGCATCGGCGAGAACGCGCTGAAGATCGCGCAGCATCTCGCGCGGCACGAAAAAGTCGAA
TGGGTCGATTATTCCGGGCTGCCGAATCATCGGGATCACGCGCTCGTCGAGCGCTACCTGTCGGGCCGCGCGCCGGGCAT
CCTGACATTCGGCGTGAAGGGCGGGCGCGCGGCGGGCGCCGCGTTCCAGGACGCGCTGAAGCTCTTCACGCGCCTCGTCA
ACATCGGCGACGCGAAGTCGCTCGCGACGCATCCCGCTTCGACGACGCATCGCCAGCTCTCGCCGGACGAGCTCGCGAAA
GCGGGCGTGAAGGAAGAGACGGTGCGGCTGTCGATCGGCATCGAGCACATCGACGATCTGCTCGCCGATCTCGATCAGGC
GCTCGCGAAGGTCTGA

Upstream 100 bases:

>100_bases
TCACCAAACAAGCTAAAAGCGCGCGGGCGCGACTCCTATAATCGGCCGACACCTCGACACGAGCAAATCGACCCACGAGC
AAATCGACCGGAGACCCATC

Downstream 100 bases:

>100_bases
AAGGCGGCGCGATCGGACGGGCGCGCGCTCGTGCGCTCGTCCGATCGTGCGATCGATCGTACGGTCCGTCGCGATGCAGC
TCGGTGCGACGCGTACGTCG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTNDVLEQRVAALEG
GIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYNLFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESV
GNPLGNITDIAALAEIAHRHGLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIERIGENALKIAQHLARHEKVE
WVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGAAFQDALKLFTRLVNIGDAKSLATHPASTTHRQLSPDELAK
AGVKEETVRLSIGIEHIDDLLADLDQALAKV

Sequences:

>Translated_431_residues
MTEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTNDVLEQRVAALEG
GIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYNLFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESV
GNPLGNITDIAALAEIAHRHGLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIERIGENALKIAQHLARHEKVE
WVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGAAFQDALKLFTRLVNIGDAKSLATHPASTTHRQLSPDELAK
AGVKEETVRLSIGIEHIDDLLADLDQALAKV
>Mature_430_residues
TEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTNDVLEQRVAALEGG
IGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYNLFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESVG
NPLGNITDIAALAEIAHRHGLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERFR
RLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIERIGENALKIAQHLARHEKVEW
VDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGAAFQDALKLFTRLVNIGDAKSLATHPASTTHRQLSPDELAKA
GVKEETVRLSIGIEHIDDLLADLDQALAKV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=404, Percent_Identity=33.6633663366337, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI299473759, Length=419, Percent_Identity=31.2649164677804, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=31.1004784688995, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790375, Length=416, Percent_Identity=29.5673076923077, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789383, Length=432, Percent_Identity=26.8518518518519, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=421, Percent_Identity=29.9287410926366, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=33.8095238095238, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=419, Percent_Identity=32.6968973747017, Blast_Score=188, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=46.7741935483871, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319307, Length=377, Percent_Identity=34.2175066312997, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=25.4504504504505, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=27.1186440677966, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=430, Percent_Identity=24.8837209302326, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=34.6793349168646, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46316; Mature: 46185

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIY
CCCCCCCCCEEEEEEEECCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCEEEEEECCHHH
SRIMNPTNDVLEQRVAALEGGIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYN
HHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCEECCCHHH
LFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESVGNPLGNITDIAALAEIAHRH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
CCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIE
HHCCCCCCCCCCEEEECCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RIGENALKIAQHLARHEKVEWVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGA
HHCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHH
AFQDALKLFTRLVNIGDAKSLATHPASTTHRQLSPDELAKAGVKEETVRLSIGIEHIDDL
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHH
LADLDQALAKV
HHHHHHHHHCC
>Mature Secondary Structure 
TEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIY
CCCCCCCCEEEEEEEECCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCEEEEEECCHHH
SRIMNPTNDVLEQRVAALEGGIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYN
HHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCEECCCHHH
LFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESVGNPLGNITDIAALAEIAHRH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
CCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIE
HHCCCCCCCCCCEEEECCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RIGENALKIAQHLARHEKVEWVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGA
HHCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHH
AFQDALKLFTRLVNIGDAKSLATHPASTTHRQLSPDELAKAGVKEETVRLSIGIEHIDDL
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHH
LADLDQALAKV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]