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The map label for this gene is prpB [H]

Identifier: 126446728

GI number: 126446728

Start: 2074350

End: 2075243

Strand: Reverse

Name: prpB [H]

Synonym: BMA10247_A2143

Alternate gene names: 126446728

Gene position: 2075243-2074350 (Counterclockwise)

Preceding gene: 126446601

Following gene: 126446197

Centisome position: 88.21

GC content: 67.34

Gene sequence:

>894_bases
ATGGGCAACCCACAACTGATCAGCGCCGGCGCGAAATTCCGCGCGGCGGTCGCGGCCGAGCAGCCGCTGCAGGTGGTCGG
CGCGATCACCGCGTACGCCGCGAAGATGGCCGAGGCGGTCGGCTTCAAGGCGGTCTATCTGTCGGGCGGCGGCGTCGCCG
CGAACTCGCTCGGCATCCCCGATCTCGGCATCAGCACGATGGACGACGTGCTCGTCGACGCGAACCGGATCACGAACGCG
ACGAACCTGCCGCTGCTCGTCGACATCGATACCGGCTGGGGCGGCGCGTTCAACATCGCGCGCACGATCCGCTCGTTCAT
CAAGGCGGGCGTGGGCGCCGTGCACCTCGAGGACCAGGTCGGTCAGAAGCGCTGCGGCCACCGCCCGGGCAAGGAATGCG
TGCCGGCGGGCGAGATGGTCGACCGGATCAAGGCGGCCGTCGACGCGCGCACCGACGAAACCTTCGTGATCATGGCGCGC
ACCGACGCCGCGGCGGCCGAGGGCATCGACGCGGCGATCGAGCGCGCGATCGCGTACGTCGAGGCGGGCGCGGACATGAT
CTTCCCCGAGGCGATGAAGACGCTCGACGACTACCGCCGCTTCAAGGAGGCGGTGAAGGTGCCGATTCTCGCGAACCTGA
CCGAGTTCGGCTCGACGCCGCTGTTCACGCTCGACGAGCTGAAGGGCGCGAACGTCGACATCGCGCTCTACTGCTGCGGC
GCGTACCGCGCGATGAACAAGGCCGCGCTGAACTTCTACGAAACGGTGCGCCGCGACGGCACGCAAAAGGCCGCGGTGCC
GACGATGCAAACGCGCGCGCAATTGTACGATTATCTCGGCTACTACGCGTACGAAGAGAAGCTGGACCAGCTTTTCAACC
AAGGCCGCAACTGA

Upstream 100 bases:

>100_bases
GTGCGCCGAATGCGGAAAGCGCGCAGAATCAGCGGGTTAGCGGCCGTCGGCACGCTTGGCCCGGACCTTGCTATTTATCG
GCATCTTTCAGGAGGAGTCG

Downstream 100 bases:

>100_bases
AGCGCCGAAGCACGGATTCAAGGATTCGAACCCCAGGAGAGTATAAGGATGAGCGAGACGAAGGAAGCAGCGGCAACGGG
CGCTGCGGGCGGTTTCAAGC

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGNPQLISAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIPDLGISTMDDVLVDANRITNA
TNLPLLVDIDTGWGGAFNIARTIRSFIKAGVGAVHLEDQVGQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMAR
TDAAAAEGIDAAIERAIAYVEAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCG
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLFNQGRN

Sequences:

>Translated_297_residues
MGNPQLISAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIPDLGISTMDDVLVDANRITNA
TNLPLLVDIDTGWGGAFNIARTIRSFIKAGVGAVHLEDQVGQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMAR
TDAAAAEGIDAAIERAIAYVEAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCG
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLFNQGRN
>Mature_296_residues
GNPQLISAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIPDLGISTMDDVLVDANRITNAT
NLPLLVDIDTGWGGAFNIARTIRSFIKAGVGAVHLEDQVGQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMART
DAAAAEGIDAAIERAIAYVEAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCGA
YRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLFNQGRN

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=288, Percent_Identity=63.8888888888889, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1790445, Length=262, Percent_Identity=28.2442748091603, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982926, Length=232, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=225, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31890; Mature: 31759

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNPQLISAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIP
CCCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCC
DLGISTMDDVLVDANRITNATNLPLLVDIDTGWGGAFNIARTIRSFIKAGVGAVHLEDQV
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHH
GQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMARTDAAAAEGIDAAIERAIAYV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCG
HCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHCCCCCCEEEEEECH
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLFNQGRN
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GNPQLISAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIP
CCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCC
DLGISTMDDVLVDANRITNATNLPLLVDIDTGWGGAFNIARTIRSFIKAGVGAVHLEDQV
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHH
GQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMARTDAAAAEGIDAAIERAIAYV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCG
HCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHCCCCCCEEEEEECH
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLFNQGRN
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]