Click here to switch to the map view.

The map label for this gene is camA [H]

Identifier: 126446234

GI number: 126446234

Start: 929216

End: 930430

Strand: Direct

Name: camA [H]

Synonym: BMA10247_A0982

Alternate gene names: 126446234

Gene position: 929216-930430 (Clockwise)

Preceding gene: 126445684

Following gene: 126445762

Centisome position: 39.5

GC content: 72.43

Gene sequence:

>1215_bases
ATGCCGCGCACCGTCGTCGTGATCGGCGGCGGCCAGGCGGCGGGCTGGGTCGTGAAGACGTTGCGCGACGAGGGCTTCGC
CGGGCGCATCGTGATGATCGCGGACGAAGCGCATCTGCCGTACGAGCGGCCTGCGCTGTCGAAGGCGGTGCTCGCGGGCG
AGGCCGACATCGCGACCGTGCGCGTCATGCAGCCCGACGCGTTCGGCGCGCTCGACGTCGACGCGTGGCAGCCGGAGCGC
GCGGCGTCGATCGATCGCGCGCGGCGCGTCGTGCGCACCGCGTCGGGCCGCGAGATCGAGTACGACCGGCTCGTGATCGC
GACGGGCGGCACGCCGCGACGCCTGCCGGATGCGATCGTCGGGACCGCTCATCTTCACTACCTGCGCACGCTCGACGAGG
CGCTCGCGCTCGGCGAGAAGCTGCGCGCGAGCCGCCGCGTGCTCGTGATCGGCGGCGGCTGGATCGGGCTCGAAGTGGCG
GCGACCGCGCGCAAGCTCGGCGTCGACGCGCTCGTCGTCGAAGGCGCGCCGCGGCTTTGCGCGCGCTCGGTGCCCGGCGC
GGTGTCCGATTTCCTGCTCGACCTGCACCGCGCAAACGGCGTCGACGTGCGGCTGAACGCGTCGCTCGCGTCGCTCGATG
CGCATCCGGACGACGCGCGCCGCGTGCGCGCGACGCTCGCGGGCGGCGGCGTCGTCGACGCGGATTTCGCGGTCGCGGGG
ATCGGTCTCGCGCTCAATACGTCGCTCGCGACCGAGGCGGGCCTGCATGTCGACGACGGCATCGTCGTCGACGAATACGG
CGCGACGAGCGATCCGGCGATCTTCGCGTGCGGCGATGTCGCGAACCATCCGAACGCGTGGCTCAAGCGGCGCGTGCGGC
TCGAATCATGGGCGAACGCGCAGCACCAGGCGATCGCGGCCGCGAAGGCGGTGCTCGGCGTGCGCGCGCCGTATGCGGAG
ATTCCGTGGTTCTGGTCCGATCAGTACGACGTGAACCTACAGATCCTCGGCGATCTGCCGGCCGACGCGCGGTGCATCGT
GCGCGGCGAGCTCGCGGCCAAGCGCGCGACGCTGTTCTTCGTCGCGGACGGCGCGCTGCGCGGGGTGATCGCGATCAACG
CGGCGCGCGAGCTGAAGCTCGCGCGCAAATGGATGAATCAGGGGCGCGCGATCGACGTCGCCGCGCTGGCGGACACGGCG
AAGGCGCTTGCCTGA

Upstream 100 bases:

>100_bases
GACGGCGACACGCGCCGGCAAAAGAACGCGCAGGCGCCGCAAGGCAAATTCATGACGGAGCGCGACGATGACTGAGCACG
CGGGTGCCGATGCGACGGCC

Downstream 100 bases:

>100_bases
GCACGAGAACGACGACATGGAGGAGAACCCACGCATGAGCACACTGGAAACCGCGATCGCGGGCGCGCCCGCGGCCGACG
CGGCGGCGGGCGCCGCGACA

Product: pyridine nucleotide-disulfide oxidoreductase, class I

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPRTVVVIGGGQAAGWVVKTLRDEGFAGRIVMIADEAHLPYERPALSKAVLAGEADIATVRVMQPDAFGALDVDAWQPER
AASIDRARRVVRTASGREIEYDRLVIATGGTPRRLPDAIVGTAHLHYLRTLDEALALGEKLRASRRVLVIGGGWIGLEVA
ATARKLGVDALVVEGAPRLCARSVPGAVSDFLLDLHRANGVDVRLNASLASLDAHPDDARRVRATLAGGGVVDADFAVAG
IGLALNTSLATEAGLHVDDGIVVDEYGATSDPAIFACGDVANHPNAWLKRRVRLESWANAQHQAIAAAKAVLGVRAPYAE
IPWFWSDQYDVNLQILGDLPADARCIVRGELAAKRATLFFVADGALRGVIAINAARELKLARKWMNQGRAIDVAALADTA
KALA

Sequences:

>Translated_404_residues
MPRTVVVIGGGQAAGWVVKTLRDEGFAGRIVMIADEAHLPYERPALSKAVLAGEADIATVRVMQPDAFGALDVDAWQPER
AASIDRARRVVRTASGREIEYDRLVIATGGTPRRLPDAIVGTAHLHYLRTLDEALALGEKLRASRRVLVIGGGWIGLEVA
ATARKLGVDALVVEGAPRLCARSVPGAVSDFLLDLHRANGVDVRLNASLASLDAHPDDARRVRATLAGGGVVDADFAVAG
IGLALNTSLATEAGLHVDDGIVVDEYGATSDPAIFACGDVANHPNAWLKRRVRLESWANAQHQAIAAAKAVLGVRAPYAE
IPWFWSDQYDVNLQILGDLPADARCIVRGELAAKRATLFFVADGALRGVIAINAARELKLARKWMNQGRAIDVAALADTA
KALA
>Mature_403_residues
PRTVVVIGGGQAAGWVVKTLRDEGFAGRIVMIADEAHLPYERPALSKAVLAGEADIATVRVMQPDAFGALDVDAWQPERA
ASIDRARRVVRTASGREIEYDRLVIATGGTPRRLPDAIVGTAHLHYLRTLDEALALGEKLRASRRVLVIGGGWIGLEVAA
TARKLGVDALVVEGAPRLCARSVPGAVSDFLLDLHRANGVDVRLNASLASLDAHPDDARRVRATLAGGGVVDADFAVAGI
GLALNTSLATEAGLHVDDGIVVDEYGATSDPAIFACGDVANHPNAWLKRRVRLESWANAQHQAIAAAKAVLGVRAPYAEI
PWFWSDQYDVNLQILGDLPADARCIVRGELAAKRATLFFVADGALRGVIAINAARELKLARKWMNQGRAIDVAALADTAK
ALA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=392, Percent_Identity=27.0408163265306, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI226437568, Length=392, Percent_Identity=27.0408163265306, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI65787454, Length=392, Percent_Identity=27.0408163265306, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4757732, Length=341, Percent_Identity=27.5659824046921, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI22202629, Length=341, Percent_Identity=27.5659824046921, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788892, Length=411, Percent_Identity=37.4695863746959, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=29.3375394321767, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789765, Length=283, Percent_Identity=28.9752650176678, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=331, Percent_Identity=28.3987915407855, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24639257, Length=328, Percent_Identity=26.5243902439024, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI281359715, Length=328, Percent_Identity=26.5243902439024, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281359713, Length=328, Percent_Identity=26.5243902439024, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24639250, Length=328, Percent_Identity=26.5243902439024, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18543267, Length=328, Percent_Identity=26.5243902439024, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24639252, Length=328, Percent_Identity=26.5243902439024, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24585130, Length=330, Percent_Identity=23.030303030303, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573993, Length=369, Percent_Identity=27.9132791327913, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24581020, Length=369, Percent_Identity=27.9132791327913, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42856; Mature: 42724

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTVVVIGGGQAAGWVVKTLRDEGFAGRIVMIADEAHLPYERPALSKAVLAGEADIATV
CCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEE
RVMQPDAFGALDVDAWQPERAASIDRARRVVRTASGREIEYDRLVIATGGTPRRLPDAIV
EEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCCCCCCHHHH
GTAHLHYLRTLDEALALGEKLRASRRVLVIGGGWIGLEVAATARKLGVDALVVEGAPRLC
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEHHHHHHCCCEEEEECCCCHHH
ARSVPGAVSDFLLDLHRANGVDVRLNASLASLDAHPDDARRVRATLAGGGVVDADFAVAG
HHCCCCHHHHHHHHHHCCCCEEEEEECCEEECCCCCCHHHHEEEEECCCCEEECCHHHEE
IGLALNTSLATEAGLHVDDGIVVDEYGATSDPAIFACGDVANHPNAWLKRRVRLESWANA
ECEEEECCHHHHCCCEECCCEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCH
QHQAIAAAKAVLGVRAPYAEIPWFWSDQYDVNLQILGDLPADARCIVRGELAAKRATLFF
HHHHHHHHHHHHHCCCCHHHCCEEECCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEEE
VADGALRGVIAINAARELKLARKWMNQGRAIDVAALADTAKALA
EECCCEEEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure 
PRTVVVIGGGQAAGWVVKTLRDEGFAGRIVMIADEAHLPYERPALSKAVLAGEADIATV
CCEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEE
RVMQPDAFGALDVDAWQPERAASIDRARRVVRTASGREIEYDRLVIATGGTPRRLPDAIV
EEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCCCCCCHHHH
GTAHLHYLRTLDEALALGEKLRASRRVLVIGGGWIGLEVAATARKLGVDALVVEGAPRLC
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEHHHHHHCCCEEEEECCCCHHH
ARSVPGAVSDFLLDLHRANGVDVRLNASLASLDAHPDDARRVRATLAGGGVVDADFAVAG
HHCCCCHHHHHHHHHHCCCCEEEEEECCEEECCCCCCHHHHEEEEECCCCEEECCHHHEE
IGLALNTSLATEAGLHVDDGIVVDEYGATSDPAIFACGDVANHPNAWLKRRVRLESWANA
ECEEEECCHHHHCCCEECCCEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCH
QHQAIAAAKAVLGVRAPYAEIPWFWSDQYDVNLQILGDLPADARCIVRGELAAKRATLFF
HHHHHHHHHHHHHCCCCHHHCCEEECCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEEE
VADGALRGVIAINAARELKLARKWMNQGRAIDVAALADTAKALA
EECCCEEEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]