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The map label for this gene is stp [H]

Identifier: 284159970

GI number: 284159970

Start: 359935

End: 361596

Strand: Reverse

Name: stp [H]

Synonym: BURPS668_A0393

Alternate gene names: 284159970

Gene position: 361596-359935 (Counterclockwise)

Preceding gene: 126443679

Following gene: 126442663

Centisome position: 11.56

GC content: 73.35

Gene sequence:

>1662_bases
ATGAATTTCTGTGGCAATGACTTGCAACAGCCGCTCAGCCTAACATGCCGCTCTCCCCTTCGCATCACGGTGTCCGGCAT
GCACGACGCGCCCTCCCGCCCCGCTCCGATCGAGCCTCCCCGTCACGCTTGCGGCTCGCCCGCCGCGTCCGCGCCGGCCC
GCGCGCGCGCGCTCGCGACCGCGTCGGCCACCTGCTCGCTGATCGTGCTCGACACGAACGTCGTCGCCGTATCGCTGCCG
TCGATCGCCCGTACGTTCCATGCGAGCTTCGCGGACATCGAATGGGTGGTGAGCGCGTACATGACCGCGTTCGCCGCATG
CCTGCTGCCGGCGGGCGGGCTCGCCGACCGCGCGGGCCGCAAGCGCGTGCTGCTCGCCGGGCTCGCGGTGTTCTTCGTCG
CGTCGCTCGGCTGCGGGCTCGCGCCGTCGGCCGCGCTCCTGAATGTCGCGCGCGCGGTCAAGGGCATCGGCGCCGCGATG
CTGCTGACCTCGGCGCTCGCCGTGATCGCGAACCGCTTCTCCGAAGGCCGCGAGCGAGCGCGCGCATGGGCGATCTGGGG
CATGTGCATGGGCGTCGCGACGGCGATCGCGCCGCTCGTCGGCGGCGCGATCGCGCAATGGATCGGGTGGCGCTGGATCT
TCCTGCTGAACCTGCCGGTGTGCATCGCGCTCGCCGCGGCCGTTCGCGCGACGATCGACGAATCGCGCGATCCGCACGCG
AAACGCATCGACGCGCCGGGCAGCGTGCTGTTCGGCGCGGCGCTCGCGCTCGGCATCTGGGCGCTGATCGACGCGCCGTC
GCACGGCTGGACCGCGCCCGGCACGCTCGCGCGCTTCGCGGCGAGCGCGGCGCTCGGCGCGGCGTTCGTCGCCGCCGAGC
GCTGGCAGCGGCGCCCGATGATCGATCTCGCGCTGTTTCGCACGCCGCGCTTCGTCGGCGCGCTGCTCGCGATGTTCGGC
TACGCGGCGTGCGCACAGGTGATGATGACGTTCCTGCCGCTCTATCTGCAGATCGGCTTCGGGATGTCGGCGATCGACGC
GGGGCTCGGCATGCTGCCGTTCGCGCTCGCGATGATCGTCGGGCCGTCGCTCGGCGCGGCGCTGTCGGCGCGCGCGCCGG
CCGCGACCGTGCTCGGCTGCGGGCTCGCGCTGATCGGCATCGGCAATTTCGCGACCGCCGCGCTCGCCGGCGCGTCGCAC
TACGGTCTCGTCGCGCTCGGGATGATGATCACCGGCTGCGGCGCGGGCATCCTCAACGGCGACACCCAGAAGGCGATCAT
GGCGTGCGTGCCGCCCGAGCGCACCGGCATGGCGTCCGGGATCAGCACGACGACGCGGTTTTCCGCGATCGTGACGTCGG
TCGGCGTGCTCGGCGCGGTGCTCGCCGAGCAGACCCACGCGGCGCTCGCCGCACGCGTCGCGCATGCGCCGGCATTGCTC
GGCGCACTCGATCCGCGCTTCATGTCGAGCCTGCTCGCGGGCGATCTCGCGCAGGCGATACGCGGCCTGCCGCCGCGCAC
CGGCGCGACGCTCGCGCACATCGCGCCCGGCGGCTTCGCGAGCGGTTTTTCGCTCGCGCTGTGCGCGAGCGGCGCGTTCG
CGCTCGCGGCGGCCGTGGCGGTGCGGCTGCTCGTCGGCGCGCAGCCGGGGCGCGCCGCATGA

Upstream 100 bases:

>100_bases
GCGAAGTGCAGGTGCCGCGCGACGCAGAGAAACGCACGCACATGGCGCAGTTCGAGGCGATCCATGGATGGCTCCTTTAT
TGCTTTTTCATGCAATAAAG

Downstream 100 bases:

>100_bases
GCGCGCGTCGAGCACGTGGGACGCGCCGCTAGCGGCGCGCCTGGCGATGCGCGGGCCGTGCAGGCCGTGCGGGCCGTTCG
AGCCATGCGAGCCGTGCGCG

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNFCGNDLQQPLSLTCRSPLRITVSGMHDAPSRPAPIEPPRHACGSPAASAPARARALATASATCSLIVLDTNVVAVSLP
SIARTFHASFADIEWVVSAYMTAFAACLLPAGGLADRAGRKRVLLAGLAVFFVASLGCGLAPSAALLNVARAVKGIGAAM
LLTSALAVIANRFSEGRERARAWAIWGMCMGVATAIAPLVGGAIAQWIGWRWIFLLNLPVCIALAAAVRATIDESRDPHA
KRIDAPGSVLFGAALALGIWALIDAPSHGWTAPGTLARFAASAALGAAFVAAERWQRRPMIDLALFRTPRFVGALLAMFG
YAACAQVMMTFLPLYLQIGFGMSAIDAGLGMLPFALAMIVGPSLGAALSARAPAATVLGCGLALIGIGNFATAALAGASH
YGLVALGMMITGCGAGILNGDTQKAIMACVPPERTGMASGISTTTRFSAIVTSVGVLGAVLAEQTHAALAARVAHAPALL
GALDPRFMSSLLAGDLAQAIRGLPPRTGATLAHIAPGGFASGFSLALCASGAFALAAAVAVRLLVGAQPGRAA

Sequences:

>Translated_553_residues
MNFCGNDLQQPLSLTCRSPLRITVSGMHDAPSRPAPIEPPRHACGSPAASAPARARALATASATCSLIVLDTNVVAVSLP
SIARTFHASFADIEWVVSAYMTAFAACLLPAGGLADRAGRKRVLLAGLAVFFVASLGCGLAPSAALLNVARAVKGIGAAM
LLTSALAVIANRFSEGRERARAWAIWGMCMGVATAIAPLVGGAIAQWIGWRWIFLLNLPVCIALAAAVRATIDESRDPHA
KRIDAPGSVLFGAALALGIWALIDAPSHGWTAPGTLARFAASAALGAAFVAAERWQRRPMIDLALFRTPRFVGALLAMFG
YAACAQVMMTFLPLYLQIGFGMSAIDAGLGMLPFALAMIVGPSLGAALSARAPAATVLGCGLALIGIGNFATAALAGASH
YGLVALGMMITGCGAGILNGDTQKAIMACVPPERTGMASGISTTTRFSAIVTSVGVLGAVLAEQTHAALAARVAHAPALL
GALDPRFMSSLLAGDLAQAIRGLPPRTGATLAHIAPGGFASGFSLALCASGAFALAAAVAVRLLVGAQPGRAA
>Mature_553_residues
MNFCGNDLQQPLSLTCRSPLRITVSGMHDAPSRPAPIEPPRHACGSPAASAPARARALATASATCSLIVLDTNVVAVSLP
SIARTFHASFADIEWVVSAYMTAFAACLLPAGGLADRAGRKRVLLAGLAVFFVASLGCGLAPSAALLNVARAVKGIGAAM
LLTSALAVIANRFSEGRERARAWAIWGMCMGVATAIAPLVGGAIAQWIGWRWIFLLNLPVCIALAAAVRATIDESRDPHA
KRIDAPGSVLFGAALALGIWALIDAPSHGWTAPGTLARFAASAALGAAFVAAERWQRRPMIDLALFRTPRFVGALLAMFG
YAACAQVMMTFLPLYLQIGFGMSAIDAGLGMLPFALAMIVGPSLGAALSARAPAATVLGCGLALIGIGNFATAALAGASH
YGLVALGMMITGCGAGILNGDTQKAIMACVPPERTGMASGISTTTRFSAIVTSVGVLGAVLAEQTHAALAARVAHAPALL
GALDPRFMSSLLAGDLAQAIRGLPPRTGATLAHIAPGGFASGFSLALCASGAFALAAAVAVRLLVGAQPGRAA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=30.6569343065693, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1790195, Length=326, Percent_Identity=30.9815950920245, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI1789042, Length=396, Percent_Identity=26.2626262626263, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=338, Percent_Identity=27.810650887574, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788710, Length=309, Percent_Identity=26.2135922330097, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790146, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=197, Percent_Identity=30.4568527918782, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=344, Percent_Identity=25, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=27.3333333333333, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=201, Percent_Identity=27.363184079602, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=196, Percent_Identity=27.0408163265306, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56055; Mature: 56055

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFCGNDLQQPLSLTCRSPLRITVSGMHDAPSRPAPIEPPRHACGSPAASAPARARALAT
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHH
ASATCSLIVLDTNVVAVSLPSIARTFHASFADIEWVVSAYMTAFAACLLPAGGLADRAGR
HCCCEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
KRVLLAGLAVFFVASLGCGLAPSAALLNVARAVKGIGAAMLLTSALAVIANRFSEGRERA
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAWAIWGMCMGVATAIAPLVGGAIAQWIGWRWIFLLNLPVCIALAAAVRATIDESRDPHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KRIDAPGSVLFGAALALGIWALIDAPSHGWTAPGTLARFAASAALGAAFVAAERWQRRPM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
IDLALFRTPRFVGALLAMFGYAACAQVMMTFLPLYLQIGFGMSAIDAGLGMLPFALAMIV
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHH
GPSLGAALSARAPAATVLGCGLALIGIGNFATAALAGASHYGLVALGMMITGCGAGILNG
CCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
DTQKAIMACVPPERTGMASGISTTTRFSAIVTSVGVLGAVLAEQTHAALAARVAHAPALL
CCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALDPRFMSSLLAGDLAQAIRGLPPRTGATLAHIAPGGFASGFSLALCASGAFALAAAVA
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHH
VRLLVGAQPGRAA
HHHHHCCCCCCCC
>Mature Secondary Structure
MNFCGNDLQQPLSLTCRSPLRITVSGMHDAPSRPAPIEPPRHACGSPAASAPARARALAT
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHH
ASATCSLIVLDTNVVAVSLPSIARTFHASFADIEWVVSAYMTAFAACLLPAGGLADRAGR
HCCCEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
KRVLLAGLAVFFVASLGCGLAPSAALLNVARAVKGIGAAMLLTSALAVIANRFSEGRERA
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAWAIWGMCMGVATAIAPLVGGAIAQWIGWRWIFLLNLPVCIALAAAVRATIDESRDPHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KRIDAPGSVLFGAALALGIWALIDAPSHGWTAPGTLARFAASAALGAAFVAAERWQRRPM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
IDLALFRTPRFVGALLAMFGYAACAQVMMTFLPLYLQIGFGMSAIDAGLGMLPFALAMIV
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHH
GPSLGAALSARAPAATVLGCGLALIGIGNFATAALAGASHYGLVALGMMITGCGAGILNG
CCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
DTQKAIMACVPPERTGMASGISTTTRFSAIVTSVGVLGAVLAEQTHAALAARVAHAPALL
CCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALDPRFMSSLLAGDLAQAIRGLPPRTGATLAHIAPGGFASGFSLALCASGAFALAAAVA
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHH
VRLLVGAQPGRAA
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]