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The map label for this gene is rsmB [H]

Identifier: 126209024

GI number: 126209024

Start: 1784135

End: 1785433

Strand: Reverse

Name: rsmB [H]

Synonym: APL_1560

Alternate gene names: 126209024

Gene position: 1785433-1784135 (Counterclockwise)

Preceding gene: 126209025

Following gene: 126209023

Centisome position: 78.5

GC content: 45.27

Gene sequence:

>1299_bases
ATGAAGAAACAAAACGCACGTGCGGTTGCCGCACAAATTATTTTACAAGTGTTGGATCAAGGTAAATCGCTTGCCACTTT
GATTCCTGAAGCTCAGGCAAAATTAGAGGCGAAAGACTTACCGCTGGTGCAAGAGATTACCTTTGGCGTGTGCCGAACCT
TACCTCGTCTTGAAGCGGTCATCGCACAAGCGGTGGAAAAACCGTTAAAAGGCAAAACTCGCTTGGTGCATTGCTTGTTA
TTAGTTGGGCTTTATCAGTTGCTTTATATGCGAGTGCCGGAATTTGCGGCGGTTGATGAAGTGGTCAATGCGGCAAGATC
GCTAAAATTGGATAATTTTAAAGCACTTATCAATGGCGTGTTACGCCGTTTTTTACGTGAAAAAGATTCGCTGTTAGCTA
AGTTTGATAAAAATTGGCAAACGCTTCACCCCGAATGGTTTGTGAATAAACTGAAAAAAGCCTATCCGAATTGGCGAGAA
ATTATCGATGCGAACAATCAACGTCCGCCGATGTGGATTCGTAGCAATATTCAGCGGATTAAACCGCAAGATTACCGTGT
CTTGTTAGGCGATCTGGTTGCAAAAAATTCGGAAAATACGACCGCTTGCGTGCCTGAATCGGCAATTTTATTAGCCAATC
CGGTACCGGTTAATAAATTGATGAATTTTGAACAAGGTTGGGCGACGGTACAAGATGCTCACGCTCAGTGGTCGGCAGCT
TTGTTGGAAGCACAAAACGGCGAATTAATTTTAGACGCTTGTGCAGCACCGGGCGGCAAAACTACGCATATTTTGGAAGC
GGCACCGCAAGCGAAGGTAATTGCGTTAGATATTGAAGAAAGCCGTCTAAAACGTGTACGTGAGAATTTAGCTCGTCTTG
GACAGACGGCAACGGTGATTTGCGGCGATGCGTCTAAACCGGCGGAATGGTTGGACGACGGTGTGATGTTTGACCGTATT
TTGCTCGATGCGCCTTGTTCCGCCACCGGCGTAATTCGCCGTCATCCGGATATTAAATGGTTACGTAAAGAAAACGATAT
TGCCGAATTAGTCGCGTTACAGGCGAAAATTCTTGAAGCATTATGGGGCAGATTAAAACCGAACGGCGTATTGGTTTATG
CGACTTGTTCGGTTCTACCGGAAGAAAACGGCGAGCAGATTACCCGTTTTGTCGCAAGCCATTCGGATGCCGAATGGGTA
GAAATGGATTTTAACGGCGAAAAAACCGCAATGAAACAGTTTTTCCCGCAAGAACAGGGCGGTGACGGTTTCTTTTATGC
GAAATTAATCAAACAATAA

Upstream 100 bases:

>100_bases
GTTCAGCTGATTGAATATTTTGACCGTTCGGGCTTTTTAAGACGAAAAGGTAATATTCATTTATTACGTGATCAAGATAC
CTTCTAATAAGAATACATTA

Downstream 100 bases:

>100_bases
ATTACCGTAAGATAAAGATGTATTTATCTTTCAGTGATTGGATTTTAAGATGAAAATTATTATTTTAGGTGCGGGTCAGG
TCGGTTCGACCCTTGCGGAA

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKKQNARAVAAQIILQVLDQGKSLATLIPEAQAKLEAKDLPLVQEITFGVCRTLPRLEAVIAQAVEKPLKGKTRLVHCLL
LVGLYQLLYMRVPEFAAVDEVVNAARSLKLDNFKALINGVLRRFLREKDSLLAKFDKNWQTLHPEWFVNKLKKAYPNWRE
IIDANNQRPPMWIRSNIQRIKPQDYRVLLGDLVAKNSENTTACVPESAILLANPVPVNKLMNFEQGWATVQDAHAQWSAA
LLEAQNGELILDACAAPGGKTTHILEAAPQAKVIALDIEESRLKRVRENLARLGQTATVICGDASKPAEWLDDGVMFDRI
LLDAPCSATGVIRRHPDIKWLRKENDIAELVALQAKILEALWGRLKPNGVLVYATCSVLPEENGEQITRFVASHSDAEWV
EMDFNGEKTAMKQFFPQEQGGDGFFYAKLIKQ

Sequences:

>Translated_432_residues
MKKQNARAVAAQIILQVLDQGKSLATLIPEAQAKLEAKDLPLVQEITFGVCRTLPRLEAVIAQAVEKPLKGKTRLVHCLL
LVGLYQLLYMRVPEFAAVDEVVNAARSLKLDNFKALINGVLRRFLREKDSLLAKFDKNWQTLHPEWFVNKLKKAYPNWRE
IIDANNQRPPMWIRSNIQRIKPQDYRVLLGDLVAKNSENTTACVPESAILLANPVPVNKLMNFEQGWATVQDAHAQWSAA
LLEAQNGELILDACAAPGGKTTHILEAAPQAKVIALDIEESRLKRVRENLARLGQTATVICGDASKPAEWLDDGVMFDRI
LLDAPCSATGVIRRHPDIKWLRKENDIAELVALQAKILEALWGRLKPNGVLVYATCSVLPEENGEQITRFVASHSDAEWV
EMDFNGEKTAMKQFFPQEQGGDGFFYAKLIKQ
>Mature_432_residues
MKKQNARAVAAQIILQVLDQGKSLATLIPEAQAKLEAKDLPLVQEITFGVCRTLPRLEAVIAQAVEKPLKGKTRLVHCLL
LVGLYQLLYMRVPEFAAVDEVVNAARSLKLDNFKALINGVLRRFLREKDSLLAKFDKNWQTLHPEWFVNKLKKAYPNWRE
IIDANNQRPPMWIRSNIQRIKPQDYRVLLGDLVAKNSENTTACVPESAILLANPVPVNKLMNFEQGWATVQDAHAQWSAA
LLEAQNGELILDACAAPGGKTTHILEAAPQAKVIALDIEESRLKRVRENLARLGQTATVICGDASKPAEWLDDGVMFDRI
LLDAPCSATGVIRRHPDIKWLRKENDIAELVALQAKILEALWGRLKPNGVLVYATCSVLPEENGEQITRFVASHSDAEWV
EMDFNGEKTAMKQFFPQEQGGDGFFYAKLIKQ

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=345, Percent_Identity=29.8550724637681, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI76150623, Length=345, Percent_Identity=29.8550724637681, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI32698918, Length=173, Percent_Identity=36.9942196531792, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI301336155, Length=177, Percent_Identity=33.8983050847458, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11545785, Length=177, Percent_Identity=30.5084745762712, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI39995082, Length=177, Percent_Identity=33.8983050847458, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI40316918, Length=215, Percent_Identity=28.8372093023256, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=435, Percent_Identity=52.8735632183908, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI87081985, Length=237, Percent_Identity=30.8016877637131, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=337, Percent_Identity=29.673590504451, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25143471, Length=217, Percent_Identity=31.7972350230415, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115534021, Length=218, Percent_Identity=30.2752293577982, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71998419, Length=218, Percent_Identity=30.2752293577982, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=294, Percent_Identity=30.6122448979592, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6319447, Length=175, Percent_Identity=31.4285714285714, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI22024126, Length=311, Percent_Identity=29.5819935691318, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24668781, Length=185, Percent_Identity=32.972972972973, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356513, Length=159, Percent_Identity=35.2201257861635, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355201, Length=199, Percent_Identity=30.1507537688442, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920494, Length=166, Percent_Identity=31.9277108433735, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48381; Mature: 48381

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQNARAVAAQIILQVLDQGKSLATLIPEAQAKLEAKDLPLVQEITFGVCRTLPRLEAV
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IAQAVEKPLKGKTRLVHCLLLVGLYQLLYMRVPEFAAVDEVVNAARSLKLDNFKALINGV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
LRRFLREKDSLLAKFDKNWQTLHPEWFVNKLKKAYPNWREIIDANNQRPPMWIRSNIQRI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHC
KPQDYRVLLGDLVAKNSENTTACVPESAILLANPVPVNKLMNFEQGWATVQDAHAQWSAA
CCCHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCHHHHHCCHHCHHHHHHHHHHHHHH
LLEAQNGELILDACAAPGGKTTHILEAAPQAKVIALDIEESRLKRVRENLARLGQTATVI
HHCCCCCCEEEEECCCCCCCCCHHEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEE
CGDASKPAEWLDDGVMFDRILLDAPCSATGVIRRHPDIKWLRKENDIAELVALQAKILEA
ECCCCCCHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHHHH
LWGRLKPNGVLVYATCSVLPEENGEQITRFVASHSDAEWVEMDFNGEKTAMKQFFPQEQG
HHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCCC
GDGFFYAKLIKQ
CCCEEHHHHHCC
>Mature Secondary Structure
MKKQNARAVAAQIILQVLDQGKSLATLIPEAQAKLEAKDLPLVQEITFGVCRTLPRLEAV
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IAQAVEKPLKGKTRLVHCLLLVGLYQLLYMRVPEFAAVDEVVNAARSLKLDNFKALINGV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
LRRFLREKDSLLAKFDKNWQTLHPEWFVNKLKKAYPNWREIIDANNQRPPMWIRSNIQRI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHC
KPQDYRVLLGDLVAKNSENTTACVPESAILLANPVPVNKLMNFEQGWATVQDAHAQWSAA
CCCHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCHHHHHCCHHCHHHHHHHHHHHHHH
LLEAQNGELILDACAAPGGKTTHILEAAPQAKVIALDIEESRLKRVRENLARLGQTATVI
HHCCCCCCEEEEECCCCCCCCCHHEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEE
CGDASKPAEWLDDGVMFDRILLDAPCSATGVIRRHPDIKWLRKENDIAELVALQAKILEA
ECCCCCCHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHHHH
LWGRLKPNGVLVYATCSVLPEENGEQITRFVASHSDAEWVEMDFNGEKTAMKQFFPQEQG
HHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCCC
GDGFFYAKLIKQ
CCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA