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The map label for this gene is dapF

Identifier: 126208995

GI number: 126208995

Start: 1745501

End: 1746325

Strand: Reverse

Name: dapF

Synonym: APL_1531

Alternate gene names: 126208995

Gene position: 1746325-1745501 (Counterclockwise)

Preceding gene: 126208997

Following gene: 126208993

Centisome position: 76.78

GC content: 45.58

Gene sequence:

>825_bases
ATGCAATTTTCAAAAATGCACGGACTTGGCAATGATTTTATGGTGATTGACGGTGTTACTCAGAACGTCTATTTAACCGA
AGAGGTGATTCGTAAACTCGCTGATCGTCATCGTGGCGTCGGCTTTGATCAGCTGTTATTGGTTGAGCCGCCTTATGATC
CGGAGCTTGATTTCCATTATCGTATCTTTAATGCGGACGGTAGCGAAGTGGCGCAATGCGGTAACGGCGCACGCTGTTTT
GCTCGCTTTGTTACGCTTAAAGGTTTAACCAATAAGCAAGATATTCACGTGAGTACCGCTAAAGGTAAAATGGTATTAAC
TTTAAAAGATGAAGAAAAAGTTCGCGTGAATATGGGCGAGCCGATTTGGGAGCCGGCACAGGTTCCGTTTACGGCAAATA
AATTTGAAAAAAACTATATTTTAAGAACCGATTTGCAAACGGTGTTATGCGGCGTGGTTTCCATGGGTAATCCGCATTGC
GTGTTGCAAGTTGAAGATATTAATCTTACACCGGTAAATGAACTCGGGCCTTTATTAGAGAATCACGAACGATTCCCCGA
GCGTGCAAATATCGGTTTTATGCAAGTGGTGAATCGCAACCATATCAAATTACGCGTATTTGAGCGCGGTGCCGGTGAAA
CGCAGGCGTGCGGTAGCGGTGCGTGCGGTGCGGTGGCGGTAGGCATTATGCAAGGCGTATTAGACAACAATGTACAGGTA
GATTTGCCCGGCGGTTCGCTCCAAATTGAGTGGGAGGGTGTTGGGCATCCGTTGTATATGACCGGCGATGCGACTCATAT
TTATGACGGGTTTATTAAACTTTAA

Upstream 100 bases:

>100_bases
AAGCGGAACGAATGCTTTGAAAAGGAACTGTAATATAGCAATAAATAAAACTAATCTCTATAAGATTTCAGAATTAAATT
TTTTTATGATAGGAAATAAT

Downstream 100 bases:

>100_bases
GTTTTTAGCTAAAAGTAAGCGGTCAAATTTCTGCAAAATTTTGCAAGAATTTGACCGCTTGTTATTTATGGCTAATCGCT
CCTAAGCGTTTTCCACACAG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVIDGVTQNVYLTEEVIRKLADRHRGVGFDQLLLVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVTLKGLTNKQDIHVSTAKGKMVLTLKDEEKVRVNMGEPIWEPAQVPFTANKFEKNYILRTDLQTVLCGVVSMGNPHC
VLQVEDINLTPVNELGPLLENHERFPERANIGFMQVVNRNHIKLRVFERGAGETQACGSGACGAVAVGIMQGVLDNNVQV
DLPGGSLQIEWEGVGHPLYMTGDATHIYDGFIKL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVIDGVTQNVYLTEEVIRKLADRHRGVGFDQLLLVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVTLKGLTNKQDIHVSTAKGKMVLTLKDEEKVRVNMGEPIWEPAQVPFTANKFEKNYILRTDLQTVLCGVVSMGNPHC
VLQVEDINLTPVNELGPLLENHERFPERANIGFMQVVNRNHIKLRVFERGAGETQACGSGACGAVAVGIMQGVLDNNVQV
DLPGGSLQIEWEGVGHPLYMTGDATHIYDGFIKL
>Mature_274_residues
MQFSKMHGLGNDFMVIDGVTQNVYLTEEVIRKLADRHRGVGFDQLLLVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVTLKGLTNKQDIHVSTAKGKMVLTLKDEEKVRVNMGEPIWEPAQVPFTANKFEKNYILRTDLQTVLCGVVSMGNPHC
VLQVEDINLTPVNELGPLLENHERFPERANIGFMQVVNRNHIKLRVFERGAGETQACGSGACGAVAVGIMQGVLDNNVQV
DLPGGSLQIEWEGVGHPLYMTGDATHIYDGFIKL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=76.6423357664234, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ACTP2 (A3N2H9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054220.1
- ProteinModelPortal:   A3N2H9
- SMR:   A3N2H9
- STRING:   A3N2H9
- GeneID:   4848681
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1531
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   A3N2H9
- ProtClustDB:   PRK00450
- BioCyc:   APLE416269:APL_1531-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30412; Mature: 30412

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVIDGVTQNVYLTEEVIRKLADRHRGVGFDQLLLVEPPYDPELDFHY
CCCHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVTLKGLTNKQDIHVSTAKGKMVLTLKDEEKVRVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCEEEEECCC
PIWEPAQVPFTANKFEKNYILRTDLQTVLCGVVSMGNPHCVLQVEDINLTPVNELGPLLE
CCCCCCCCCEECCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
NHERFPERANIGFMQVVNRNHIKLRVFERGAGETQACGSGACGAVAVGIMQGVLDNNVQV
HHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
DLPGGSLQIEWEGVGHPLYMTGDATHIYDGFIKL
EECCCEEEEEEECCCCCEEEECCCCEEECCEEEC
>Mature Secondary Structure
MQFSKMHGLGNDFMVIDGVTQNVYLTEEVIRKLADRHRGVGFDQLLLVEPPYDPELDFHY
CCCHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVTLKGLTNKQDIHVSTAKGKMVLTLKDEEKVRVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCEEEEECCC
PIWEPAQVPFTANKFEKNYILRTDLQTVLCGVVSMGNPHCVLQVEDINLTPVNELGPLLE
CCCCCCCCCEECCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
NHERFPERANIGFMQVVNRNHIKLRVFERGAGETQACGSGACGAVAVGIMQGVLDNNVQV
HHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
DLPGGSLQIEWEGVGHPLYMTGDATHIYDGFIKL
EECCCEEEEEEECCCCCEEEECCCCEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA