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The map label for this gene is frp [H]

Identifier: 126207967

GI number: 126207967

Start: 552582

End: 553301

Strand: Direct

Name: frp [H]

Synonym: APL_0483

Alternate gene names: 126207967

Gene position: 552582-553301 (Clockwise)

Preceding gene: 126207966

Following gene: 126207968

Centisome position: 24.29

GC content: 45.28

Gene sequence:

>720_bases
ATGATGAGCAAACCAACCTTAGAAACGATACTTTCGCATCGTTCTATTCGTAAATTTACCGAACAACCTATCTCGGAAGA
TCTATTCCAAACCTTAATCCAAGCCGGGCAACAAGCCTCAACCTCCAATCATTTGCAATGCGTGAGCGTGATTCGTGTCA
AAGATCAAACCATACGCCAAGCATTGCGTGAGGTATCGGGTATGGCATATGTTACCGAATGTGCGGAGTTCCTAGTTTTT
TGCATTGATTTTCATAAACATAAGCAGCTTGTGCCGGATTCGCAATTGGATTGGGCGGAAGTCAGCGTAATCGGTGCGGT
GGATACCGGAATTTTTGCCCAAAATGTTTTGCTGGCTGCGGAATCGGTCGGCTTAGGCGGAGTATATATCGGGGCATTAC
GTAATGATGTGGCGAAAGTGGCGGAAGTATTAGGGTTACCGCAATATTGTGTGCCGTTAGTAGGAATGTGTTTGGGTTAT
CCGGCACAGGATCCCGCACTAAAACCGCGTTTGCCTATAAGTTTGGTCTGTTACGAAGATCAATATCAGCCGTTGGATCA
AACGGAACTTACAAAATATAACGAAATTTTGACCGCTTATTATCAGGCGAGAACCGGTGAAGGACAGCAGTGGCAAGCGG
CGATTGACAAAACCTTAAATAAAGCGGTGCGTCCGCATATGTTACCGTTTTTCCAACAACAAGGATTGTTGAAGCGATGA

Upstream 100 bases:

>100_bases
TTTTACACCAATTTTATTGCGAATAGTTTGATTTTTACGTCACTGCCACATAAGATTGAAAAAACGTGTAATGTCGGACG
TTTATTTTATCGGAGGCAAT

Downstream 100 bases:

>100_bases
AATTTTTAATGTTGTGTCGTGAGCCTCGTTTATACAGTTGCCAACGTTTACAACAGGCTTGTGAGCAACGGGGAATTAGC
TTAGATATTCTCGACCCGAA

Product: nitroreductase A

Products: NA

Alternate protein names: Flavin reductase P; NADPH-FMN oxidoreductase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MMSKPTLETILSHRSIRKFTEQPISEDLFQTLIQAGQQASTSNHLQCVSVIRVKDQTIRQALREVSGMAYVTECAEFLVF
CIDFHKHKQLVPDSQLDWAEVSVIGAVDTGIFAQNVLLAAESVGLGGVYIGALRNDVAKVAEVLGLPQYCVPLVGMCLGY
PAQDPALKPRLPISLVCYEDQYQPLDQTELTKYNEILTAYYQARTGEGQQWQAAIDKTLNKAVRPHMLPFFQQQGLLKR

Sequences:

>Translated_239_residues
MMSKPTLETILSHRSIRKFTEQPISEDLFQTLIQAGQQASTSNHLQCVSVIRVKDQTIRQALREVSGMAYVTECAEFLVF
CIDFHKHKQLVPDSQLDWAEVSVIGAVDTGIFAQNVLLAAESVGLGGVYIGALRNDVAKVAEVLGLPQYCVPLVGMCLGY
PAQDPALKPRLPISLVCYEDQYQPLDQTELTKYNEILTAYYQARTGEGQQWQAAIDKTLNKAVRPHMLPFFQQQGLLKR
>Mature_239_residues
MMSKPTLETILSHRSIRKFTEQPISEDLFQTLIQAGQQASTSNHLQCVSVIRVKDQTIRQALREVSGMAYVTECAEFLVF
CIDFHKHKQLVPDSQLDWAEVSVIGAVDTGIFAQNVLLAAESVGLGGVYIGALRNDVAKVAEVLGLPQYCVPLVGMCLGY
PAQDPALKPRLPISLVCYEDQYQPLDQTELTKYNEILTAYYQARTGEGQQWQAAIDKTLNKAVRPHMLPFFQQQGLLKR

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=238, Percent_Identity=47.0588235294118, Blast_Score=222, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26685; Mature: 26685

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKPTLETILSHRSIRKFTEQPISEDLFQTLIQAGQQASTSNHLQCVSVIRVKDQTIRQ
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ALREVSGMAYVTECAEFLVFCIDFHKHKQLVPDSQLDWAEVSVIGAVDTGIFAQNVLLAA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHH
ESVGLGGVYIGALRNDVAKVAEVLGLPQYCVPLVGMCLGYPAQDPALKPRLPISLVCYED
HHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
QYQPLDQTELTKYNEILTAYYQARTGEGQQWQAAIDKTLNKAVRPHMLPFFQQQGLLKR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMSKPTLETILSHRSIRKFTEQPISEDLFQTLIQAGQQASTSNHLQCVSVIRVKDQTIRQ
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ALREVSGMAYVTECAEFLVFCIDFHKHKQLVPDSQLDWAEVSVIGAVDTGIFAQNVLLAA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHH
ESVGLGGVYIGALRNDVAKVAEVLGLPQYCVPLVGMCLGYPAQDPALKPRLPISLVCYED
HHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
QYQPLDQTELTKYNEILTAYYQARTGEGQQWQAAIDKTLNKAVRPHMLPFFQQQGLLKR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206832; 8885832 [H]