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The map label for this gene is gapA [H]

Identifier: 126207918

GI number: 126207918

Start: 490520

End: 491524

Strand: Direct

Name: gapA [H]

Synonym: APL_0434

Alternate gene names: 126207918

Gene position: 490520-491524 (Clockwise)

Preceding gene: 126207916

Following gene: 126207919

Centisome position: 21.57

GC content: 41.19

Gene sequence:

>1005_bases
ATGGCTATTAAAATTGGTATTAACGGCTTCGGTCGTATCGGTCGTATCGTGTTCCGTGCAGCGCAACTTCGTGATGATAT
CGAAGTTGTAGGTATCAACGACTTAATCGACGTTGATTACATGGCTTACATGTTAAAATACGATTCAACTCACGGTCGTT
TCAATGGTACAGTGGAAGTTAAAGACGGTCAATTAGTAGTTAATGGTAAAGCGATTCGCGTAACTTCCGAGCGTGATCCT
GCTAACTTAAAATGGAATGAAATCGGTGTTGATATCGCAGTTGAAGCAACCGGTTTATTCTTAGACGACGCAACAGCACG
TAAACACATTACTGCAGGTGCGAAAAAAGTTGTTTTAACCGGTCCGTCTAAAGATGCAACACCTATGTTCGTTAATGGTG
TAAACTTCGATGCATACGCAGGCCAAGATATCGTTTCTAACGCTTCTTGTACAACTAACTGCTTAGCACCGTTAGCTAAA
GTCATCCACAATAAATTCGGTATTAAAGAAGGTTTAATGACTACAGTTCACGCAACAACTGCGACACAAAAAACTGTAGA
TGGTCCATCAGCGAAAGACTGGCGCGGTGGTCGTGGTGCTTCACAAAACATCATTCCTTCATCAACAGGTGCGGCGAAAG
CGGTAGGTAAAGTATTACCTGCATTAAACGGTAAATTAACCGGTATGGCTTTCCGTGTTCCGACAGCAAACGTTTCTGTA
GTTGATTTAACAGTGAACTTAGAAAAACCTGCTACATACGAAGAAATCTGTGCGGAAGTTAAACGCGCTTCACAAGAAGA
AATGAAAGGCGTGTTAGGTTATACTGAAGATGCGGTAGTATCAACAGACTTTAACGGTGCGGTTGAAACTTCTGTATTTG
ATGCGAAAGCAGGTATCGCATTAACGAATACTTTCGTTAAGTTAGTATCTTGGTACGATAACGAAACCGGTTACTCAAAC
AAAGTATTAGATTTAGTCGCTCACGTTTATAACTACAAAGGCTAA

Upstream 100 bases:

>100_bases
TTTAGTTGAAAGGCATATTAGAGTATGTGCTATAATCCTTGCACTTTCAGAGTATGGAAGAACGAATTTTGTTTAACTTT
AAATATAGGTGAAAAAATCT

Downstream 100 bases:

>100_bases
TTAAGTTAGTCGCTTATTGCATACGCCACTCCAAAAGAGTGGCGTTTTTTTGTTTAAATGCAAGTAATTTTCTTTTGTCT
AAATTATTCATTGAAGTGTA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAIKIGINGFGRIGRIVFRAAQLRDDIEVVGINDLIDVDYMAYMLKYDSTHGRFNGTVEVKDGQLVVNGKAIRVTSERDP
ANLKWNEIGVDIAVEATGLFLDDATARKHITAGAKKVVLTGPSKDATPMFVNGVNFDAYAGQDIVSNASCTTNCLAPLAK
VIHNKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTANVSV
VDLTVNLEKPATYEEICAEVKRASQEEMKGVLGYTEDAVVSTDFNGAVETSVFDAKAGIALTNTFVKLVSWYDNETGYSN
KVLDLVAHVYNYKG

Sequences:

>Translated_334_residues
MAIKIGINGFGRIGRIVFRAAQLRDDIEVVGINDLIDVDYMAYMLKYDSTHGRFNGTVEVKDGQLVVNGKAIRVTSERDP
ANLKWNEIGVDIAVEATGLFLDDATARKHITAGAKKVVLTGPSKDATPMFVNGVNFDAYAGQDIVSNASCTTNCLAPLAK
VIHNKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTANVSV
VDLTVNLEKPATYEEICAEVKRASQEEMKGVLGYTEDAVVSTDFNGAVETSVFDAKAGIALTNTFVKLVSWYDNETGYSN
KVLDLVAHVYNYKG
>Mature_333_residues
AIKIGINGFGRIGRIVFRAAQLRDDIEVVGINDLIDVDYMAYMLKYDSTHGRFNGTVEVKDGQLVVNGKAIRVTSERDPA
NLKWNEIGVDIAVEATGLFLDDATARKHITAGAKKVVLTGPSKDATPMFVNGVNFDAYAGQDIVSNASCTTNCLAPLAKV
IHNKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTANVSVV
DLTVNLEKPATYEEICAEVKRASQEEMKGVLGYTEDAVVSTDFNGAVETSVFDAKAGIALTNTFVKLVSWYDNETGYSNK
VLDLVAHVYNYKG

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=329, Percent_Identity=64.1337386018237, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI7657116, Length=332, Percent_Identity=60.8433734939759, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=85.1063829787234, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=40.7294832826748, Blast_Score=250, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17568413, Length=333, Percent_Identity=61.8618618618619, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32566163, Length=333, Percent_Identity=61.8618618618619, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17534677, Length=333, Percent_Identity=61.2612612612613, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17534679, Length=333, Percent_Identity=60.6606606606607, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322409, Length=329, Percent_Identity=67.1732522796352, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321631, Length=329, Percent_Identity=66.8693009118541, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322468, Length=329, Percent_Identity=66.2613981762918, Blast_Score=431, Evalue=1e-122,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=66.4634146341463, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=66.4634146341463, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=66.1585365853659, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=66.1585365853659, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=62.5766871165644, Blast_Score=409, Evalue=1e-114,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35714; Mature: 35583

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKIGINGFGRIGRIVFRAAQLRDDIEVVGINDLIDVDYMAYMLKYDSTHGRFNGTVEV
CEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEECCCCCEECCEEEE
KDGQLVVNGKAIRVTSERDPANLKWNEIGVDIAVEATGLFLDDATARKHITAGAKKVVLT
ECCEEEECCEEEEEECCCCCCCCEEHHCCEEEEEEEEEEEEECCHHHHHHCCCCCEEEEE
GPSKDATPMFVNGVNFDAYAGQDIVSNASCTTNCLAPLAKVIHNKFGIKEGLMTTVHATT
CCCCCCCCEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
ATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTANVSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
VDLTVNLEKPATYEEICAEVKRASQEEMKGVLGYTEDAVVSTDFNGAVETSVFDAKAGIA
EEEEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEECCCCCCEECEEEECCCCCH
LTNTFVKLVSWYDNETGYSNKVLDLVAHVYNYKG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIKIGINGFGRIGRIVFRAAQLRDDIEVVGINDLIDVDYMAYMLKYDSTHGRFNGTVEV
EEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEECCCCCEECCEEEE
KDGQLVVNGKAIRVTSERDPANLKWNEIGVDIAVEATGLFLDDATARKHITAGAKKVVLT
ECCEEEECCEEEEEECCCCCCCCEEHHCCEEEEEEEEEEEEECCHHHHHHCCCCCEEEEE
GPSKDATPMFVNGVNFDAYAGQDIVSNASCTTNCLAPLAKVIHNKFGIKEGLMTTVHATT
CCCCCCCCEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
ATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTANVSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
VDLTVNLEKPATYEEICAEVKRASQEEMKGVLGYTEDAVVSTDFNGAVETSVFDAKAGIA
EEEEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEECCCCCCEECEEEECCCCCH
LTNTFVKLVSWYDNETGYSNKVLDLVAHVYNYKG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]