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The map label for this gene is leuB [H]

Identifier: 126207916

GI number: 126207916

Start: 488696

End: 489778

Strand: Direct

Name: leuB [H]

Synonym: APL_0432

Alternate gene names: 126207916

Gene position: 488696-489778 (Clockwise)

Preceding gene: 126207915

Following gene: 126207918

Centisome position: 21.49

GC content: 43.21

Gene sequence:

>1083_bases
ATGCAAACTTACAACATTGCGGTTTTAGCGGGGGACGGTATCGGTCCTGAAATTATGGCACAAGCGATTAAAGTGCTTGA
AGCAACGCAGCAAAAATTCGGTTTTAAATTAAATTTCAATCATTTTAATATCGGCGGTGCGGCAATTGACGCACAAGGTA
AAGCGTTGCCGGAAAATACTTTAAAAGGTTGTGAAGAAGCCGATGCGATTCTGTTTGGTTCTGTGGGCGGGCCTAAATGG
GTACATTTACCGCCGGACGAACAACCGGAGCGTGGTTCATTACTTCCGTTACGTAAACATTTCAAATTATTCTGTAACTT
ACGTCCTGCAACTTTATATAAAGGCTTAGAAAAATTCTGTCCGTTACGCGCGGATATTGCGGCGAAAGGCTTCGATATGG
TTGTCGTACGTGAATTAACCGGCGGGATTTATTTCGGACAGCCGAAAGGGCGTGAAGGCGAAGGTTCGCAAACCAAAGCG
TTTGATACCGAAGTGTATTACAAATACGAGATTGAACGTATTGCACGCGTTGCTTTTGAATCTGCAATGAAACGTAATAA
AAAAGTAACGTCCATTGATAAAGCAAACGTATTACAGAGCTCGATTTTATGGCGTGAAACGGTCAGCGAAATTGCGAAAG
AATATCCGGAAGTCACGCTTGAGCATATGTATATTGACAATGCGACAATGCAATTAATCAAAGCGCCGGAAACCTTTGAT
ATCTTATTATGTTCGAATATTTTCGGCGATATTATTTCGGATGAGGCGGCAATGATTACCGGTTCGATGGGGATGTTGCC
GTCTGCGAGTTTAAATGAAGACGGTTTCGGTTTATATGAACCGGCTGGCGGTTCGGCACCGGATATTGCAGGTAAGAATA
TTGCGAACCCAATCGCACAAATCTTATCGGCGGCAATGATGTTACGTTATAGCTTCAATTTAAATGAGGCGGCGGATGCG
ATTGAAGCGGCGATTCAAAAAGCATTAGCGGACGGTTATCGCACCGGTGATTTAGCGGATGAAAACACGCCGATTTCTAC
CAGTGAAATGGGCGATATTATTGCGAGTAATATTTTATCGTAA

Upstream 100 bases:

>100_bases
CACACGGCATTCTAAATCAAATTTCATCATTTCCCACCCAATAACCGAAATTTTAAGGTAGAATGTTTGAATCTTTACAT
AACAATAAGAGGATATTTAT

Downstream 100 bases:

>100_bases
CTAGTGAATTATTTCGTATAAACAAGCGGTCGAAATTTGAAAATAATTTGCAAAAATTCTTCAAATTTCGACCGCTTGTC
TTTATGCCTAATGTAGAAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MQTYNIAVLAGDGIGPEIMAQAIKVLEATQQKFGFKLNFNHFNIGGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKW
VHLPPDEQPERGSLLPLRKHFKLFCNLRPATLYKGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGREGEGSQTKA
FDTEVYYKYEIERIARVAFESAMKRNKKVTSIDKANVLQSSILWRETVSEIAKEYPEVTLEHMYIDNATMQLIKAPETFD
ILLCSNIFGDIISDEAAMITGSMGMLPSASLNEDGFGLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLNEAADA
IEAAIQKALADGYRTGDLADENTPISTSEMGDIIASNILS

Sequences:

>Translated_360_residues
MQTYNIAVLAGDGIGPEIMAQAIKVLEATQQKFGFKLNFNHFNIGGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKW
VHLPPDEQPERGSLLPLRKHFKLFCNLRPATLYKGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGREGEGSQTKA
FDTEVYYKYEIERIARVAFESAMKRNKKVTSIDKANVLQSSILWRETVSEIAKEYPEVTLEHMYIDNATMQLIKAPETFD
ILLCSNIFGDIISDEAAMITGSMGMLPSASLNEDGFGLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLNEAADA
IEAAIQKALADGYRTGDLADENTPISTSEMGDIIASNILS
>Mature_360_residues
MQTYNIAVLAGDGIGPEIMAQAIKVLEATQQKFGFKLNFNHFNIGGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKW
VHLPPDEQPERGSLLPLRKHFKLFCNLRPATLYKGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGREGEGSQTKA
FDTEVYYKYEIERIARVAFESAMKRNKKVTSIDKANVLQSSILWRETVSEIAKEYPEVTLEHMYIDNATMQLIKAPETFD
ILLCSNIFGDIISDEAAMITGSMGMLPSASLNEDGFGLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLNEAADA
IEAAIQKALADGYRTGDLADENTPISTSEMGDIIASNILS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=331, Percent_Identity=31.7220543806647, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI28178816, Length=342, Percent_Identity=29.2397660818713, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178821, Length=347, Percent_Identity=29.1066282420749, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758582, Length=356, Percent_Identity=28.0898876404494, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=325, Percent_Identity=28.3076923076923, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28178819, Length=177, Percent_Identity=36.1581920903955, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=73.9495798319328, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=39.8373983739837, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=25.8706467661692, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=353, Percent_Identity=31.728045325779, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=354, Percent_Identity=29.9435028248588, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=335, Percent_Identity=30.7462686567164, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=344, Percent_Identity=28.1976744186047, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=48.9010989010989, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6322097, Length=370, Percent_Identity=34.0540540540541, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=31.1572700296736, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=30.5949008498584, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=34.6938775510204, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=34.6938775510204, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24661184, Length=364, Percent_Identity=31.5934065934066, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI161078635, Length=353, Percent_Identity=28.6118980169972, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078637, Length=353, Percent_Identity=28.6118980169972, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078633, Length=353, Percent_Identity=28.8951841359773, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650122, Length=353, Percent_Identity=28.8951841359773, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078639, Length=351, Percent_Identity=29.0598290598291, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281362242, Length=341, Percent_Identity=27.5659824046921, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24648872, Length=341, Percent_Identity=27.5659824046921, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20130355, Length=341, Percent_Identity=24.9266862170088, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39118; Mature: 39118

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTYNIAVLAGDGIGPEIMAQAIKVLEATQQKFGFKLNFNHFNIGGAAIDAQGKALPENT
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCEEECCCEEECCCCCCCCHHH
LKGCEEADAILFGSVGGPKWVHLPPDEQPERGSLLPLRKHFKLFCNLRPATLYKGLEKFC
HCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
PLRADIAAKGFDMVVVRELTGGIYFGQPKGREGEGSQTKAFDTEVYYKYEIERIARVAFE
CCHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHH
SAMKRNKKVTSIDKANVLQSSILWRETVSEIAKEYPEVTLEHMYIDNATMQLIKAPETFD
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEECCHHHHHHCCCCCHH
ILLCSNIFGDIISDEAAMITGSMGMLPSASLNEDGFGLYEPAGGSAPDIAGKNIANPIAQ
HEEHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHH
ILSAAMMLRYSFNLNEAADAIEAAIQKALADGYRTGDLADENTPISTSEMGDIIASNILS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTYNIAVLAGDGIGPEIMAQAIKVLEATQQKFGFKLNFNHFNIGGAAIDAQGKALPENT
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCEEECCCEEECCCCCCCCHHH
LKGCEEADAILFGSVGGPKWVHLPPDEQPERGSLLPLRKHFKLFCNLRPATLYKGLEKFC
HCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
PLRADIAAKGFDMVVVRELTGGIYFGQPKGREGEGSQTKAFDTEVYYKYEIERIARVAFE
CCHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHH
SAMKRNKKVTSIDKANVLQSSILWRETVSEIAKEYPEVTLEHMYIDNATMQLIKAPETFD
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEECCHHHHHHCCCCCHH
ILLCSNIFGDIISDEAAMITGSMGMLPSASLNEDGFGLYEPAGGSAPDIAGKNIANPIAQ
HEEHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHH
ILSAAMMLRYSFNLNEAADAIEAAIQKALADGYRTGDLADENTPISTSEMGDIIASNILS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA