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The map label for this gene is ribA

Identifier: 126207868

GI number: 126207868

Start: 437672

End: 438877

Strand: Direct

Name: ribA

Synonym: APL_0384

Alternate gene names: 126207868

Gene position: 437672-438877 (Clockwise)

Preceding gene: 126207867

Following gene: 126207869

Centisome position: 19.24

GC content: 41.87

Gene sequence:

>1206_bases
ATGACAGATTTCCAATTTTCAAAAGTAGAAGATGCGATCGAAGCGATTCGACAAGGCAAAATCATTTTAGTGACTGACGA
TGAAGATCGCGAAAACGAAGGCGATTTTATCTGTGCGGCGGAATTTGCCACACCGGAAAATATCAATTTTATGGCAACTT
ACGGCAAAGGTTTGATTTGTACGCCGATTTCAACCGAAATCGCTAAAAAATTAAATTTCCATCCGATGGTTGCGGTCAAT
CAAGATAATCATGAAACGGCGTTTACCGTATCGGTGGATCATATTGATACGGGAACGGGTATCTCAGCTTTTGAGCGTTC
GATTACCGCAATGAAAATTGTCGATGATAATGCTAAAGCAACGGATTTCCGCCGCCCGGGGCATATGTTTCCGTTAATCG
CTAAAGAAGGTGGAGTGTTAGTGCGTAACGGTCATACCGAAGCAACAGTGGATTTAGCTCGTTTAGCCGGTTTAAAACAC
GCCGGTTTATGTTGTGAAATTATGGCGGATGACGGCACGATGATGACTATGCCGGATCTACAAAAATTTGCGGTAGAACA
CAATATGCCGTTTATCACGATTCAACAATTACAAGAATATCGCCGTAAGCATGACAGCTTGGTGAAACAAATTTCTGTGG
TAAAAATGCCGACAAAATACGGTGAGTTTATGGCACATAGCTTTGTTGAAGTGATTTCAGGTAAAGAACACGTTGCGTTA
GTCAAAGGCGATTTAACCGACGGTGAGCAAGTATTGGCGCGTATCCATTCGGAATGTTTAACCGGTGACGCTTTCGGTTC
TCAACGTTGTGATTGCGGTCAGCAATTTGCCGCAGCAATGACCCAAATTGAGCAAGAGGGCAGAGGTGTGATTCTGTATT
TACGCCAAGAAGGTCGTGGTATCGGTTTAATCAATAAGCTACGTGCTTACGAACTACAAGATAAAGGGATGGATACCGTT
GAAGCGAACGTCGCTTTAGGATTTAAAGAAGACGAACGTGAGTACTATATCGGTGCACAAATGTTCCAGCAGTTAGGCGT
AAAATCGATCCGTTTATTAACCAATAATCCGGCAAAAATTGAAGGCTTAAAAGAGCAAGGATTAAATATCGTTGCACGTG
AGCCGATTATTGTAGAACCGAACAAAAATGACATTGATTACCTAAAAGTCAAACAGATAAAAATGGGGCATATGTTTAAC
TTCTAA

Upstream 100 bases:

>100_bases
TAATCTTAGTTTAGACTTTTTAAAGCAGGCGGGATTTTAAGATTTGTAGGACACACTGAGTGTATCCTACCGACAAAAAT
ATATATTTTAGGAAAAGAAG

Downstream 100 bases:

>100_bases
CTTTAACAACCGTATGTAGTATTAGGGAAGCAAGCGTTGCGTCCCTACTATAGAATGATACAAGCGGTCACTTTTTTATA
AAATTTTGCATATTTCGAGA

Product: riboflavin biosynthesis protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTDFQFSKVEDAIEAIRQGKIILVTDDEDRENEGDFICAAEFATPENINFMATYGKGLICTPISTEIAKKLNFHPMVAVN
QDNHETAFTVSVDHIDTGTGISAFERSITAMKIVDDNAKATDFRRPGHMFPLIAKEGGVLVRNGHTEATVDLARLAGLKH
AGLCCEIMADDGTMMTMPDLQKFAVEHNMPFITIQQLQEYRRKHDSLVKQISVVKMPTKYGEFMAHSFVEVISGKEHVAL
VKGDLTDGEQVLARIHSECLTGDAFGSQRCDCGQQFAAAMTQIEQEGRGVILYLRQEGRGIGLINKLRAYELQDKGMDTV
EANVALGFKEDEREYYIGAQMFQQLGVKSIRLLTNNPAKIEGLKEQGLNIVAREPIIVEPNKNDIDYLKVKQIKMGHMFN
F

Sequences:

>Translated_401_residues
MTDFQFSKVEDAIEAIRQGKIILVTDDEDRENEGDFICAAEFATPENINFMATYGKGLICTPISTEIAKKLNFHPMVAVN
QDNHETAFTVSVDHIDTGTGISAFERSITAMKIVDDNAKATDFRRPGHMFPLIAKEGGVLVRNGHTEATVDLARLAGLKH
AGLCCEIMADDGTMMTMPDLQKFAVEHNMPFITIQQLQEYRRKHDSLVKQISVVKMPTKYGEFMAHSFVEVISGKEHVAL
VKGDLTDGEQVLARIHSECLTGDAFGSQRCDCGQQFAAAMTQIEQEGRGVILYLRQEGRGIGLINKLRAYELQDKGMDTV
EANVALGFKEDEREYYIGAQMFQQLGVKSIRLLTNNPAKIEGLKEQGLNIVAREPIIVEPNKNDIDYLKVKQIKMGHMFN
F
>Mature_400_residues
TDFQFSKVEDAIEAIRQGKIILVTDDEDRENEGDFICAAEFATPENINFMATYGKGLICTPISTEIAKKLNFHPMVAVNQ
DNHETAFTVSVDHIDTGTGISAFERSITAMKIVDDNAKATDFRRPGHMFPLIAKEGGVLVRNGHTEATVDLARLAGLKHA
GLCCEIMADDGTMMTMPDLQKFAVEHNMPFITIQQLQEYRRKHDSLVKQISVVKMPTKYGEFMAHSFVEVISGKEHVALV
KGDLTDGEQVLARIHSECLTGDAFGSQRCDCGQQFAAAMTQIEQEGRGVILYLRQEGRGIGLINKLRAYELQDKGMDTVE
ANVALGFKEDEREYYIGAQMFQQLGVKSIRLLTNNPAKIEGLKEQGLNIVAREPIIVEPNKNDIDYLKVKQIKMGHMFNF

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=57.5129533678757, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=45.771144278607, Blast_Score=185, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320695, Length=208, Percent_Identity=41.8269230769231, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6319438, Length=170, Percent_Identity=44.1176470588235, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_ACTP2 (A3MZA2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053093.1
- ProteinModelPortal:   A3MZA2
- STRING:   A3MZA2
- GeneID:   4850491
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0384
- eggNOG:   COG0108
- HOGENOM:   HBG735778
- OMA:   NIVAREP
- PhylomeDB:   A3MZA2
- ProtClustDB:   CLSK918908
- BioCyc:   APLE416269:APL_0384-MONOMER
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 44741; Mature: 44610

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 331-331 ACT_SITE 333-333 BINDING 35-35 BINDING 166-166 BINDING 275-275 BINDING 319-319 BINDING 354-354 BINDING 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFQFSKVEDAIEAIRQGKIILVTDDEDRENEGDFICAAEFATPENINFMATYGKGLIC
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEE
TPISTEIAKKLNFHPMVAVNQDNHETAFTVSVDHIDTGTGISAFERSITAMKIVDDNAKA
CCCCHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCCC
TDFRRPGHMFPLIAKEGGVLVRNGHTEATVDLARLAGLKHAGLCCEIMADDGTMMTMPDL
CCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCHH
QKFAVEHNMPFITIQQLQEYRRKHDSLVKQISVVKMPTKYGEFMAHSFVEVISGKEHVAL
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCEEEE
VKGDLTDGEQVLARIHSECLTGDAFGSQRCDCGQQFAAAMTQIEQEGRGVILYLRQEGRG
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCC
IGLINKLRAYELQDKGMDTVEANVALGFKEDEREYYIGAQMFQQLGVKSIRLLTNNPAKI
CCHHHHHHHEECCCCCCCEEEEEEEECCCCCCCCEEHHHHHHHHCCCCEEEEEECCCHHC
EGLKEQGLNIVAREPIIVEPNKNDIDYLKVKQIKMGHMFNF
CCHHHCCCEEEECCCEEECCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
TDFQFSKVEDAIEAIRQGKIILVTDDEDRENEGDFICAAEFATPENINFMATYGKGLIC
CCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEE
TPISTEIAKKLNFHPMVAVNQDNHETAFTVSVDHIDTGTGISAFERSITAMKIVDDNAKA
CCCCHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCCC
TDFRRPGHMFPLIAKEGGVLVRNGHTEATVDLARLAGLKHAGLCCEIMADDGTMMTMPDL
CCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCHH
QKFAVEHNMPFITIQQLQEYRRKHDSLVKQISVVKMPTKYGEFMAHSFVEVISGKEHVAL
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCEEEE
VKGDLTDGEQVLARIHSECLTGDAFGSQRCDCGQQFAAAMTQIEQEGRGVILYLRQEGRG
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCC
IGLINKLRAYELQDKGMDTVEANVALGFKEDEREYYIGAQMFQQLGVKSIRLLTNNPAKI
CCHHHHHHHEECCCCCCCEEEEEEEECCCCCCCCEEHHHHHHHHCCCCEEEEEECCCHHC
EGLKEQGLNIVAREPIIVEPNKNDIDYLKVKQIKMGHMFNF
CCHHHCCCEEEECCCEEECCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA