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The map label for this gene is ribH

Identifier: 126207869

GI number: 126207869

Start: 438988

End: 439449

Strand: Direct

Name: ribH

Synonym: APL_0385

Alternate gene names: 126207869

Gene position: 438988-439449 (Clockwise)

Preceding gene: 126207868

Following gene: 126207874

Centisome position: 19.3

GC content: 40.91

Gene sequence:

>462_bases
ATGGCAAAGATTACAGGTAACTTAGTTGCGACAGGTTTAAAATTCGGTATTGTAACCGCACGTTTCAACGATTTTATCAA
CGATAAATTATTAAGCGGTGCAATTGATACGTTAGTGCGTCACGGTGCGTATGAAAACGATATTGATACGGCATGGGTTC
CGGGTGCATTTGAGATTCCATTAGTTGCGAAAAAAATGGCAACCAGCGGTAAATATGATGCGGTAATCTGTTTAGGTACG
GTAATTAGCGGTTCGACAACTCACTATGATTACGTATGTAATGAAGCGGCAAAAGGTATCGGTGCGGTAGCATTAGAAAC
CGGCGTACCGGTAATTTTCGGTGTATTAACCACAGAAAATATTGAACAGGCGATTGAACGCGCGGGTACTAAAGCAGGTA
ATAAAGGTTCAGAATGTGCATTAGGCGCAATCGAAATAGTAAACGTATTAAAAGCGATCTAA

Upstream 100 bases:

>100_bases
CGTATGTAGTATTAGGGAAGCAAGCGTTGCGTCCCTACTATAGAATGATACAAGCGGTCACTTTTTTATAAAATTTTGCA
TATTTCGAGAGGACAAAAAA

Downstream 100 bases:

>100_bases
TTTTCGTTTGACGTGCTAAAAACAAGCGGTCGTTTTTGACTGGAATTTTGCAAATTTCCCGTTAAAAACGACCGCTTATA
TTTTATGTCTAGTAAAGACC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MAKITGNLVATGLKFGIVTARFNDFINDKLLSGAIDTLVRHGAYENDIDTAWVPGAFEIPLVAKKMATSGKYDAVICLGT
VISGSTTHYDYVCNEAAKGIGAVALETGVPVIFGVLTTENIEQAIERAGTKAGNKGSECALGAIEIVNVLKAI

Sequences:

>Translated_153_residues
MAKITGNLVATGLKFGIVTARFNDFINDKLLSGAIDTLVRHGAYENDIDTAWVPGAFEIPLVAKKMATSGKYDAVICLGT
VISGSTTHYDYVCNEAAKGIGAVALETGVPVIFGVLTTENIEQAIERAGTKAGNKGSECALGAIEIVNVLKAI
>Mature_152_residues
AKITGNLVATGLKFGIVTARFNDFINDKLLSGAIDTLVRHGAYENDIDTAWVPGAFEIPLVAKKMATSGKYDAVICLGTV
ISGSTTHYDYVCNEAAKGIGAVALETGVPVIFGVLTTENIEQAIERAGTKAGNKGSECALGAIEIVNVLKAI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=57.7922077922078, Blast_Score=175, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324429, Length=145, Percent_Identity=35.1724137931034, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_ACTP2 (A3MZA3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053094.1
- ProteinModelPortal:   A3MZA3
- SMR:   A3MZA3
- STRING:   A3MZA3
- GeneID:   4850446
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0385
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   A3MZA3
- ProtClustDB:   PRK00061
- BioCyc:   APLE416269:APL_0385-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 15961; Mature: 15829

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITGNLVATGLKFGIVTARFNDFINDKLLSGAIDTLVRHGAYENDIDTAWVPGAFEIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
LVAKKMATSGKYDAVICLGTVISGSTTHYDYVCNEAAKGIGAVALETGVPVIFGVLTTEN
HHHHHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHCCCCEEEHHCCCCEEEHHHHHHH
IEQAIERAGTKAGNKGSECALGAIEIVNVLKAI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKITGNLVATGLKFGIVTARFNDFINDKLLSGAIDTLVRHGAYENDIDTAWVPGAFEIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
LVAKKMATSGKYDAVICLGTVISGSTTHYDYVCNEAAKGIGAVALETGVPVIFGVLTTEN
HHHHHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHCCCCEEEHHCCCCEEEHHHHHHH
IEQAIERAGTKAGNKGSECALGAIEIVNVLKAI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA