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The map label for this gene is ribE [H]

Identifier: 126207867

GI number: 126207867

Start: 436964

End: 437611

Strand: Direct

Name: ribE [H]

Synonym: APL_0383

Alternate gene names: 126207867

Gene position: 436964-437611 (Clockwise)

Preceding gene: 126207866

Following gene: 126207868

Centisome position: 19.21

GC content: 40.59

Gene sequence:

>648_bases
ATGTTCACAGGTATTATTGAAGAAGTCGGCAAAATTGCTCAAATTCATAAGCAAGGCGAATTTGCGGTAGTCACAATTAA
TGCGACCAAAGTATTACAAGACGTTCATTTAGGCGACAGCATTGCGGTGAACGGCGTATGTTTAACCGTAACTTCTTTTT
CGAGTAATCAGTTTACCGCCGATGTAATGTCGGAAACGTTAAAACGTACTTCATTAGGCGAATTAAAGTCGAATAGTCCG
GTTAATTTAGAACGCGCGATGGCGGCAAACGGACGTTTCGGCGGACACATCGTTTCGGGGCATATTGACGGCACCGGCGA
AATTGCGGAAATCACACCGGCACATAATTCGACATGGTATCGCATTAAAACCTCTCCAAAATTAATGCGTTATATTATTG
AGAAAGGTTCGATCACCATTGACGGTATTAGCCTGACCGTAGTCGATACCGATGATGAAAGTTTCCGTGTATCGATTATT
CCGCATACGATTAAAGAAACCAATTTAGGTTCGAAAAAAATCGGCAGTATTGTCAATTTAGAAAATGATATTGTCGGTAA
ATATATCGAACAGTTTTTACTGAAAAAGCCGGCGGATGAGCCGAAAAGTAATCTTAGTTTAGACTTTTTAAAGCAGGCGG
GATTTTAA

Upstream 100 bases:

>100_bases
TGAGATAATGTTGAAATTTACACCGCCTTTCACTTTGGCGTTGTTAAATCTCCCCTAACCCCTCTTTACAAAAGAGAGGG
ATCAATAATGAGGAAATTAT

Downstream 100 bases:

>100_bases
GATTTGTAGGACACACTGAGTGTATCCTACCGACAAAAATATATATTTTAGGAAAAGAAGATGACAGATTTCCAATTTTC
AAAAGTAGAAGATGCGATCG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MFTGIIEEVGKIAQIHKQGEFAVVTINATKVLQDVHLGDSIAVNGVCLTVTSFSSNQFTADVMSETLKRTSLGELKSNSP
VNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHNSTWYRIKTSPKLMRYIIEKGSITIDGISLTVVDTDDESFRVSII
PHTIKETNLGSKKIGSIVNLENDIVGKYIEQFLLKKPADEPKSNLSLDFLKQAGF

Sequences:

>Translated_215_residues
MFTGIIEEVGKIAQIHKQGEFAVVTINATKVLQDVHLGDSIAVNGVCLTVTSFSSNQFTADVMSETLKRTSLGELKSNSP
VNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHNSTWYRIKTSPKLMRYIIEKGSITIDGISLTVVDTDDESFRVSII
PHTIKETNLGSKKIGSIVNLENDIVGKYIEQFLLKKPADEPKSNLSLDFLKQAGF
>Mature_215_residues
MFTGIIEEVGKIAQIHKQGEFAVVTINATKVLQDVHLGDSIAVNGVCLTVTSFSSNQFTADVMSETLKRTSLGELKSNSP
VNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHNSTWYRIKTSPKLMRYIIEKGSITIDGISLTVVDTDDESFRVSII
PHTIKETNLGSKKIGSIVNLENDIVGKYIEQFLLKKPADEPKSNLSLDFLKQAGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=42.4731182795699, Blast_Score=141, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319733, Length=206, Percent_Identity=41.747572815534, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23390; Mature: 23390

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIEEVGKIAQIHKQGEFAVVTINATKVLQDVHLGDSIAVNGVCLTVTSFSSNQFTA
CCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCCCEEECCEEEEEEECCCCCEEH
DVMSETLKRTSLGELKSNSPVNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHNSTWY
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCEEE
RIKTSPKLMRYIIEKGSITIDGISLTVVDTDDESFRVSIIPHTIKETNLGSKKIGSIVNL
EEECCHHHHHHHHHCCCEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHEECC
ENDIVGKYIEQFLLKKPADEPKSNLSLDFLKQAGF
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MFTGIIEEVGKIAQIHKQGEFAVVTINATKVLQDVHLGDSIAVNGVCLTVTSFSSNQFTA
CCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCCCEEECCEEEEEEECCCCCEEH
DVMSETLKRTSLGELKSNSPVNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHNSTWY
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCEEE
RIKTSPKLMRYIIEKGSITIDGISLTVVDTDDESFRVSIIPHTIKETNLGSKKIGSIVNL
EEECCHHHHHHHHHCCCEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHEECC
ENDIVGKYIEQFLLKKPADEPKSNLSLDFLKQAGF
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]