Click here to switch to the map view.

The map label for this gene is ribD [H]

Identifier: 126207866

GI number: 126207866

Start: 435609

End: 436841

Strand: Direct

Name: ribD [H]

Synonym: APL_0382

Alternate gene names: 126207866

Gene position: 435609-436841 (Clockwise)

Preceding gene: 126207865

Following gene: 126207867

Centisome position: 19.15

GC content: 44.04

Gene sequence:

>1233_bases
ATGAAATTACCGTGTAAGCGGTGGTTTTTCCTATCTTTTTTACAAGCCTTGAGATCGAAAGATTTCAAGGCTTTTTTCAT
CATTAGGGTAAACATGCCTGTAATGTGTTTTCCTCTGCCCTCAAATAGTTTCAAAACAATGACGGATTTAGACTATATGC
GCCGTGCCATTGCACTGGCAAAACAAGGTTTAGGCTGGACGAATCCCAATCCGCTTGTCGGTTGTGTAATTGTCAAAAAC
GGTGAAATCGTTGCCGAAGGTTACCATGAAAAGATTGGTGGATGGCATGCGGAACGTAATGCCGTTTTACATTGTAAGGA
AGATCTTTCCGGGGCGACTGCTTATGTAACGCTTGAGCCTTGTTGTCATCACGGCCGCACGCCGCCTTGTTCGGATTTAT
TAATTGAACGAGGCATTAAAAAAGTATTTATCGGTTCGAGCGATCCGAATCCTTTAGTAGCAGGGCGGGGAGCAAATCAG
CTACGCCAAGCCGGCGTGGAAGTGGTGGAAGGTTTACTCAAAGAAGAATGTGATGCGTTAAACCCGATTTTTTTCCACTA
TATTCAAACTAAACGTCCGTATGTGCTAATGAAATATGCCATGACGGCAGACGGCAAAATTGCAACCGGTAGCGGCGAAT
CCAAATGGATTACCGGTGAATCGGCAAGAGCAAGAGTGCAGCAAACACGTCATCAATATAGTGCGATTATGGTCGGTGTA
GATACGGTACTTGCCGATAACCCGATGTTAAATAGCCGAATGCCGAATGCGAAACAACCGGTCCGGATTGTCTGCGATAG
CCAATTACGTACACCGTTAGATTGCCAGTTAGTGCAGACAGCGAAAGAATATCGCACCGTAATTGCAACCGTTAGTGACG
ATTTGCAAAAAATTGAACAATTTAGACCGCTTGGCGTAGATGTATTAGTGTGTAAAGCACGAAACAAGCGGGTAGATTTG
CAAGATCTTTTGCAAAAGCTCGGTGAAATGCAGATCGACAGCCTCTTATTGGAAGGCGGTTCAAGTTTGAATTTCAGTGC
GTTAGAAAGCGGTATCGTGAATCGAGTACATTGTTATATTGCGCCTAAATTAGTCGGTGGTAAACAAGCGAAAACCCCAA
TCGGCGGTGAGGGAATTCAACAAATCGACCAAGCGGTTAAATTAAAATTGAAATCGACCGAACTCATCGGCGAAGATATT
TTGTTGGATTATGTAGTCATCTCCCCTCTTTAG

Upstream 100 bases:

>100_bases
GAAATTCCCGATCGGCGGTAAAGTCCGCGAGCCGAACGAAAAAGGTTTGGCAGGAACCGGTGAGATTCCGGTACCGACAG
TATAGTCTGGATGGAAGAAG

Downstream 100 bases:

>100_bases
CAAAGAGGGGTCGGGGGAGATTTGAGATAATGTTGAAATTTACACCGCCTTTCACTTTGGCGTTGTTAAATCTCCCCTAA
CCCCTCTTTACAAAAGAGAG

Product: riboflavin biosynthesis protein

Products: NA

Alternate protein names: Diaminohydroxyphosphoribosylaminopyrimidine deaminase; DRAP deaminase; Riboflavin-specific deaminase; 5-amino-6-(5-phosphoribosylamino)uracil reductase; HTP reductase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKLPCKRWFFLSFLQALRSKDFKAFFIIRVNMPVMCFPLPSNSFKTMTDLDYMRRAIALAKQGLGWTNPNPLVGCVIVKN
GEIVAEGYHEKIGGWHAERNAVLHCKEDLSGATAYVTLEPCCHHGRTPPCSDLLIERGIKKVFIGSSDPNPLVAGRGANQ
LRQAGVEVVEGLLKEECDALNPIFFHYIQTKRPYVLMKYAMTADGKIATGSGESKWITGESARARVQQTRHQYSAIMVGV
DTVLADNPMLNSRMPNAKQPVRIVCDSQLRTPLDCQLVQTAKEYRTVIATVSDDLQKIEQFRPLGVDVLVCKARNKRVDL
QDLLQKLGEMQIDSLLLEGGSSLNFSALESGIVNRVHCYIAPKLVGGKQAKTPIGGEGIQQIDQAVKLKLKSTELIGEDI
LLDYVVISPL

Sequences:

>Translated_410_residues
MKLPCKRWFFLSFLQALRSKDFKAFFIIRVNMPVMCFPLPSNSFKTMTDLDYMRRAIALAKQGLGWTNPNPLVGCVIVKN
GEIVAEGYHEKIGGWHAERNAVLHCKEDLSGATAYVTLEPCCHHGRTPPCSDLLIERGIKKVFIGSSDPNPLVAGRGANQ
LRQAGVEVVEGLLKEECDALNPIFFHYIQTKRPYVLMKYAMTADGKIATGSGESKWITGESARARVQQTRHQYSAIMVGV
DTVLADNPMLNSRMPNAKQPVRIVCDSQLRTPLDCQLVQTAKEYRTVIATVSDDLQKIEQFRPLGVDVLVCKARNKRVDL
QDLLQKLGEMQIDSLLLEGGSSLNFSALESGIVNRVHCYIAPKLVGGKQAKTPIGGEGIQQIDQAVKLKLKSTELIGEDI
LLDYVVISPL
>Mature_410_residues
MKLPCKRWFFLSFLQALRSKDFKAFFIIRVNMPVMCFPLPSNSFKTMTDLDYMRRAIALAKQGLGWTNPNPLVGCVIVKN
GEIVAEGYHEKIGGWHAERNAVLHCKEDLSGATAYVTLEPCCHHGRTPPCSDLLIERGIKKVFIGSSDPNPLVAGRGANQ
LRQAGVEVVEGLLKEECDALNPIFFHYIQTKRPYVLMKYAMTADGKIATGSGESKWITGESARARVQQTRHQYSAIMVGV
DTVLADNPMLNSRMPNAKQPVRIVCDSQLRTPLDCQLVQTAKEYRTVIATVSDDLQKIEQFRPLGVDVLVCKARNKRVDL
QDLLQKLGEMQIDSLLLEGGSSLNFSALESGIVNRVHCYIAPKLVGGKQAKTPIGGEGIQQIDQAVKLKLKSTELIGEDI
LLDYVVISPL

Specific function: Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate [H]

COG id: COG1985

COG function: function code H; Pyrimidine reductase, riboflavin biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the HTP reductase family [H]

Homologues:

Organism=Escherichia coli, GI1786616, Length=372, Percent_Identity=42.2043010752688, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI145693172, Length=147, Percent_Identity=34.0136054421769, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319629, Length=184, Percent_Identity=29.8913043478261, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193
- InterPro:   IPR004794
- InterPro:   IPR011549
- InterPro:   IPR002734 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1; PF01872 RibD_C [H]

EC number: =3.5.4.26; =1.1.1.193 [H]

Molecular weight: Translated: 45440; Mature: 45440

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPCKRWFFLSFLQALRSKDFKAFFIIRVNMPVMCFPLPSNSFKTMTDLDYMRRAIALA
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHH
KQGLGWTNPNPLVGCVIVKNGEIVAEGYHEKIGGWHAERNAVLHCKEDLSGATAYVTLEP
HHCCCCCCCCCEEEEEEEECCCEEECCHHHHHCCEECCCCCEEEECCCCCCCEEEEEECH
CCHHGRTPPCSDLLIERGIKKVFIGSSDPNPLVAGRGANQLRQAGVEVVEGLLKEECDAL
HHCCCCCCCHHHHHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHC
NPIFFHYIQTKRPYVLMKYAMTADGKIATGSGESKWITGESARARVQQTRHQYSAIMVGV
CHHHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHEEEH
DTVLADNPMLNSRMPNAKQPVRIVCDSQLRTPLDCQLVQTAKEYRTVIATVSDDLQKIEQ
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FRPLGVDVLVCKARNKRVDLQDLLQKLGEMQIDSLLLEGGSSLNFSALESGIVNRVHCYI
HCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEEE
APKLVGGKQAKTPIGGEGIQQIDQAVKLKLKSTELIGEDILLDYVVISPL
ECEECCCCCCCCCCCCHHHHHHHHHHHEEEHHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MKLPCKRWFFLSFLQALRSKDFKAFFIIRVNMPVMCFPLPSNSFKTMTDLDYMRRAIALA
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHH
KQGLGWTNPNPLVGCVIVKNGEIVAEGYHEKIGGWHAERNAVLHCKEDLSGATAYVTLEP
HHCCCCCCCCCEEEEEEEECCCEEECCHHHHHCCEECCCCCEEEECCCCCCCEEEEEECH
CCHHGRTPPCSDLLIERGIKKVFIGSSDPNPLVAGRGANQLRQAGVEVVEGLLKEECDAL
HHCCCCCCCHHHHHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHC
NPIFFHYIQTKRPYVLMKYAMTADGKIATGSGESKWITGESARARVQQTRHQYSAIMVGV
CHHHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHEEEH
DTVLADNPMLNSRMPNAKQPVRIVCDSQLRTPLDCQLVQTAKEYRTVIATVSDDLQKIEQ
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FRPLGVDVLVCKARNKRVDLQDLLQKLGEMQIDSLLLEGGSSLNFSALESGIVNRVHCYI
HCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEEE
APKLVGGKQAKTPIGGEGIQQIDQAVKLKLKSTELIGEDILLDYVVISPL
ECEECCCCCCCCCCCCHHHHHHHHHHHEEEHHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]