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The map label for this gene is grcA

Identifier: 126207845

GI number: 126207845

Start: 402489

End: 402875

Strand: Reverse

Name: grcA

Synonym: APL_0361

Alternate gene names: 126207845

Gene position: 402875-402489 (Counterclockwise)

Preceding gene: 126207847

Following gene: 126207844

Centisome position: 17.71

GC content: 40.83

Gene sequence:

>387_bases
ATGATCAAAGGCGTACAAATTACAGAATCTTCAAACAGCAACTTAGTAAACTCTTTCTGGTTATTAGACGAAGAGAAAAA
CGAAGCGCGTTGTATCGCGGCTAAAGGCGACGTTTATAAAGAAGACCAAGTTATCGCAATCAGCGAATTAGGTCAAATCG
CATACCGCGAAGTGCCGGTAAACGTTGCTCCGACAATCAAAGTTGAAGGCGGTCAGCACTTAAACGTTAATGTTTTACGT
CGTGAAACATTAGAAGATGCGGTTAAAAACCCGGAAAAATATCCGCAATTAACCATCCGTGTTTCAGGTTATGCAGTGCG
TTTCAACTCATTAACACCGGAACAACAACGTGACGTAATCACTCGTACTTTCACAGAAAGTTTATAA

Upstream 100 bases:

>100_bases
TAGAAATCTTCAAAAAAAGTGATAAAATCCACTGCATCAACAAAATTTGAATTTATCAAAAATTTTTATAATTATCCAGC
CAATACTCAGGAGTATCAAA

Downstream 100 bases:

>100_bases
TAACAGCTTTACTGTTCATTTTCTTAAAAAAGCACCTTCGGGTGCTTTTTTATTGCTTCTGTTTTACAAATCTTCCCTCA
AATTCCACCGCTTGCTAAAT

Product: autonomous glycyl radical cofactor GrcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MIKGVQITESSNSNLVNSFWLLDEEKNEARCIAAKGDVYKEDQVIAISELGQIAYREVPVNVAPTIKVEGGQHLNVNVLR
RETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL

Sequences:

>Translated_128_residues
MIKGVQITESSNSNLVNSFWLLDEEKNEARCIAAKGDVYKEDQVIAISELGQIAYREVPVNVAPTIKVEGGQHLNVNVLR
RETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL
>Mature_128_residues
MIKGVQITESSNSNLVNSFWLLDEEKNEARCIAAKGDVYKEDQVIAISELGQIAYREVPVNVAPTIKVEGGQHLNVNVLR
RETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain

Homologues:

Organism=Escherichia coli, GI1788933, Length=128, Percent_Identity=78.125, Blast_Score=211, Evalue=8e-57,
Organism=Escherichia coli, GI1787131, Length=61, Percent_Identity=81.9672131147541, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI48994926, Length=61, Percent_Identity=77.0491803278689, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1787044, Length=58, Percent_Identity=44.8275862068966, Blast_Score=63, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRCA_ACTP2 (A3MZ79)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053070.1
- ProteinModelPortal:   A3MZ79
- SMR:   A3MZ79
- STRING:   A3MZ79
- GeneID:   4850301
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0361
- eggNOG:   COG3445
- HOGENOM:   HBG297207
- OMA:   GQFEYRE
- PhylomeDB:   A3MZ79
- ProtClustDB:   PRK11127
- BioCyc:   APLE416269:APL_0361-MONOMER
- HAMAP:   MF_00806
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140
- PIRSF:   PIRSF000378

Pfam domain/function: PF01228 Gly_radical

EC number: NA

Molecular weight: Translated: 14434; Mature: 14434

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGVQITESSNSNLVNSFWLLDEEKNEARCIAAKGDVYKEDQVIAISELGQIAYREVPV
CCCCEEEECCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCEEEHHHCCCCEEEECCC
NVAPTIKVEGGQHLNVNVLRRETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVI
CCCCEEEECCCCEEEEEEEHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHH
TRTFTESL
HHHHHHCC
>Mature Secondary Structure
MIKGVQITESSNSNLVNSFWLLDEEKNEARCIAAKGDVYKEDQVIAISELGQIAYREVPV
CCCCEEEECCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCEEEHHHCCCCEEEECCC
NVAPTIKVEGGQHLNVNVLRRETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVI
CCCCEEEECCCCEEEEEEEHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHH
TRTFTESL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA