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The map label for this gene is proP [H]

Identifier: 126207839

GI number: 126207839

Start: 397874

End: 399070

Strand: Direct

Name: proP [H]

Synonym: APL_0355

Alternate gene names: 126207839

Gene position: 397874-399070 (Clockwise)

Preceding gene: 126207838

Following gene: 126207842

Centisome position: 17.49

GC content: 43.61

Gene sequence:

>1197_bases
ATGGTAACACAAAGACCGCATCCGTGCTTTTTTATTATTCTCGGTATGATGGCGATGTTACCGCCTTTAGCGATTGATAT
GTATTTGCCGTCGTTTCTTGATATCGCGAGAGATTTACAAGTTTCACAGGAAAAAGTACAAACCACATTGGCTGTTTTTA
CCTTCGGTTTTGCCGTAGGGCAACTTTTTTGGGGACCGATGGCGGACAGCTTCGGACGTAAGCCGATTATTTTGTTCGGT
TTAACGGGCTCCGCTTTAGCCGCCTATTTCCTGACACAAGTCATGACTATTGAAAACTTTTATTTGCTTCGACTGATACA
AGGACTTTGTGCGGCGGCGCCGGCGGTAGTATTGGGAGCCTTAGTACGAGATCTGTTTGATCGTAATATGTTTGCACAAA
TGATGTCGGTCATTATGATTATTTCGATGCTTGCGCCGTTGTTAGCACCGATTTTAGGCGGTTATATTGCGAAATACTTC
CATTGGCACTCTATTTTTTATACGTTGGTGTTAATGGGCATAAGTTGCGTGCTACTTATCAGTTGGAAGATTCCGGAAAC
CTTAGCGATTGAAAAACGACAACCGCTGAGATTCGGCATTGTTTTTAAAAACTTTTGGACGTTGCTATCCGATAAAGCGA
CCTTAGGTTATGTTTTAGTCGGCGGTTTAACGTTTGCCGGAATGTTCTGTTTCTTAACCTCCGGATCGTTGGTTTACATC
GGTATTTATGGCGTTTCGCAAGAGTATTTCGGCTACTTCTTCGGTTTGAATATGATTGTAATGGTCTCTATGACCGCATT
AAACGGACGGATTGTGGTTAAAGTCGGCTCGGAAAAAATGTTGAAAATCGGCTTGCTTGTTCAATTGTTAGCCGGTATTT
GGTTGGCGTGCGTTGCAATTTTTAAATTAGGTTTTTGGGCGATGGCAATCGGTATTCCGTTTTATGTCGGCATGCTTTCG
ACTATCGGTAGTAATGCGACTGCAGCGATTTTAGATCGTTATCCGCAAATGGCGGGAACGGCAAACGGTTTAGCCGGAAC
CGCACGCTTCGGTATCGCTTCTTTAGTCGGTGCAATGTTATCGCACATTGCCGTTACGAGCGAACGCCCTATGCTATATG
CTATGGCGATGTGTACGATAGCCGCTTCTATAGCTTATTACCTACTATGTAAAAATAAGTCGAAACAAGCGATCTGA

Upstream 100 bases:

>100_bases
AAGCGTTACATCGTTGGCGTATCGGTGATGTGGTTTTAGACGAAAATCTTGAAGAAGGCGAATATCGTCCGCTTAGCGAA
CAAGAAGTGGAGAGTTTTTA

Downstream 100 bases:

>100_bases
TTTTATGTAAAAACGGAAAAGCGGACACTTAAGTGTCCGCTTTTTTTACCAACAGGCATAACAGTAATGCCAAGGATGGA
AGCGAGTGCGCCCAAAATCG

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MVTQRPHPCFFIILGMMAMLPPLAIDMYLPSFLDIARDLQVSQEKVQTTLAVFTFGFAVGQLFWGPMADSFGRKPIILFG
LTGSALAAYFLTQVMTIENFYLLRLIQGLCAAAPAVVLGALVRDLFDRNMFAQMMSVIMIISMLAPLLAPILGGYIAKYF
HWHSIFYTLVLMGISCVLLISWKIPETLAIEKRQPLRFGIVFKNFWTLLSDKATLGYVLVGGLTFAGMFCFLTSGSLVYI
GIYGVSQEYFGYFFGLNMIVMVSMTALNGRIVVKVGSEKMLKIGLLVQLLAGIWLACVAIFKLGFWAMAIGIPFYVGMLS
TIGSNATAAILDRYPQMAGTANGLAGTARFGIASLVGAMLSHIAVTSERPMLYAMAMCTIAASIAYYLLCKNKSKQAI

Sequences:

>Translated_398_residues
MVTQRPHPCFFIILGMMAMLPPLAIDMYLPSFLDIARDLQVSQEKVQTTLAVFTFGFAVGQLFWGPMADSFGRKPIILFG
LTGSALAAYFLTQVMTIENFYLLRLIQGLCAAAPAVVLGALVRDLFDRNMFAQMMSVIMIISMLAPLLAPILGGYIAKYF
HWHSIFYTLVLMGISCVLLISWKIPETLAIEKRQPLRFGIVFKNFWTLLSDKATLGYVLVGGLTFAGMFCFLTSGSLVYI
GIYGVSQEYFGYFFGLNMIVMVSMTALNGRIVVKVGSEKMLKIGLLVQLLAGIWLACVAIFKLGFWAMAIGIPFYVGMLS
TIGSNATAAILDRYPQMAGTANGLAGTARFGIASLVGAMLSHIAVTSERPMLYAMAMCTIAASIAYYLLCKNKSKQAI
>Mature_398_residues
MVTQRPHPCFFIILGMMAMLPPLAIDMYLPSFLDIARDLQVSQEKVQTTLAVFTFGFAVGQLFWGPMADSFGRKPIILFG
LTGSALAAYFLTQVMTIENFYLLRLIQGLCAAAPAVVLGALVRDLFDRNMFAQMMSVIMIISMLAPLLAPILGGYIAKYF
HWHSIFYTLVLMGISCVLLISWKIPETLAIEKRQPLRFGIVFKNFWTLLSDKATLGYVLVGGLTFAGMFCFLTSGSLVYI
GIYGVSQEYFGYFFGLNMIVMVSMTALNGRIVVKVGSEKMLKIGLLVQLLAGIWLACVAIFKLGFWAMAIGIPFYVGMLS
TIGSNATAAILDRYPQMAGTANGLAGTARFGIASLVGAMLSHIAVTSERPMLYAMAMCTIAASIAYYLLCKNKSKQAI

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=397, Percent_Identity=43.3249370277078, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI48994889, Length=374, Percent_Identity=31.283422459893, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1790146, Length=256, Percent_Identity=30.078125, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082312, Length=330, Percent_Identity=24.8484848484848, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790794, Length=194, Percent_Identity=25.2577319587629, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787065, Length=185, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321838, Length=241, Percent_Identity=26.9709543568465, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=171, Percent_Identity=30.4093567251462, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43572; Mature: 43572

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]