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The map label for this gene is nfo

Identifier: 126207842

GI number: 126207842

Start: 400200

End: 401042

Strand: Direct

Name: nfo

Synonym: APL_0358

Alternate gene names: 126207842

Gene position: 400200-401042 (Clockwise)

Preceding gene: 126207839

Following gene: 126207843

Centisome position: 17.6

GC content: 39.26

Gene sequence:

>843_bases
ATGAAATATATTGGTGCGCACGTGAGTGCTTCGGGCGGTGTGGAGAACGCCGTATTAAGAGCGGTTGAAATCGGCGCAAA
TGCTTTTGCATTATTTACTAAAAACCAACGTCAATGGAAAGCACTGGCATTAAAGGCGGATACGATTGAAAAATTTAAGC
GTTTTTGTAAAGCGCATCAATTTTCGCCGGAGCATATTTTACCGCACGACAGTTACTTAATTAATTTAGGTAATCCTGAA
GCGGAAAATTTAGCCAAATCACGCGAAGCCTTTATCGATGAAATGGAACGGGCAAATCAGTTAGGATTAAAATTACTGAA
TTTTCACCCCAGCGCACATTTAAATAAAATTTCGGAAAGCGAATGTTTAGCCCGAATTGCCGAGTCGATTAATATTGCGG
TAGATAAAGTGCCTAATGTGATTGCGGTAATTGAGAATACTGCCGGTCAAGGTTCCAATCTCGGTTATCGTTTTGAACAT
TTAGCCGAAATTATTGACCAAGTAGAGGATAAAAACCGAGTCGGTGTTTGTTTAGATACTTGTCATCTCTTTTCAGCAGG
TTATGACATCAGTTCTTTAGAGTCTTGCGAACAAACATTTTCCGAATTTGAACGGACGGTCGGTTTTCAGTATTTACGAG
GTATGCACTTAAACGGTTCAAAAACGCCGTTAGGTAGCCGAGTAGATCGACATCACACATTAAGAGAAGGCACAATTGGA
ACGGATTTTTGCAAATTTATCATGCAAGACGACCGCTTTGACAATATTCCGCTTATTTTAGAAACAATTCAGCCTGAAAT
TTGGACAGAAGAAATAAAATTTTTGCGGACATTAGCAAAATAA

Upstream 100 bases:

>100_bases
AACGGCAGAGAAGTGAGAAATCGTTAATGGTTGTTTCGCTCATAAGTAATGATTTATAAAGGTTAAGAAGTTAGTCATTT
TACACGAATAGGATAAGAAG

Downstream 100 bases:

>100_bases
TCCGTTATACTGCCAGAGTTTGATTGTTGCGACCTAGGTAAAATAGGTCGCCAAATTTATTAACTTTAAGATTTTTAATA
GGAATCAACCGCTGTAGTTG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKYIGAHVSASGGVENAVLRAVEIGANAFALFTKNQRQWKALALKADTIEKFKRFCKAHQFSPEHILPHDSYLINLGNPE
AENLAKSREAFIDEMERANQLGLKLLNFHPSAHLNKISESECLARIAESINIAVDKVPNVIAVIENTAGQGSNLGYRFEH
LAEIIDQVEDKNRVGVCLDTCHLFSAGYDISSLESCEQTFSEFERTVGFQYLRGMHLNGSKTPLGSRVDRHHTLREGTIG
TDFCKFIMQDDRFDNIPLILETIQPEIWTEEIKFLRTLAK

Sequences:

>Translated_280_residues
MKYIGAHVSASGGVENAVLRAVEIGANAFALFTKNQRQWKALALKADTIEKFKRFCKAHQFSPEHILPHDSYLINLGNPE
AENLAKSREAFIDEMERANQLGLKLLNFHPSAHLNKISESECLARIAESINIAVDKVPNVIAVIENTAGQGSNLGYRFEH
LAEIIDQVEDKNRVGVCLDTCHLFSAGYDISSLESCEQTFSEFERTVGFQYLRGMHLNGSKTPLGSRVDRHHTLREGTIG
TDFCKFIMQDDRFDNIPLILETIQPEIWTEEIKFLRTLAK
>Mature_280_residues
MKYIGAHVSASGGVENAVLRAVEIGANAFALFTKNQRQWKALALKADTIEKFKRFCKAHQFSPEHILPHDSYLINLGNPE
AENLAKSREAFIDEMERANQLGLKLLNFHPSAHLNKISESECLARIAESINIAVDKVPNVIAVIENTAGQGSNLGYRFEH
LAEIIDQVEDKNRVGVCLDTCHLFSAGYDISSLESCEQTFSEFERTVGFQYLRGMHLNGSKTPLGSRVDRHHTLREGTIG
TDFCKFIMQDDRFDNIPLILETIQPEIWTEEIKFLRTLAK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=276, Percent_Identity=68.4782608695652, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17531193, Length=279, Percent_Identity=42.6523297491039, Blast_Score=257, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6322735, Length=278, Percent_Identity=40.6474820143885, Blast_Score=227, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_ACTP2 (A3MZ76)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053067.1
- ProteinModelPortal:   A3MZ76
- SMR:   A3MZ76
- STRING:   A3MZ76
- GeneID:   4849433
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0358
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   A3MZ76
- ProtClustDB:   PRK01060
- BioCyc:   APLE416269:APL_0358-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31519; Mature: 31519

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGAHVSASGGVENAVLRAVEIGANAFALFTKNQRQWKALALKADTIEKFKRFCKAHQ
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
FSPEHILPHDSYLINLGNPEAENLAKSREAFIDEMERANQLGLKLLNFHPSAHLNKISES
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHHHCCHH
ECLARIAESINIAVDKVPNVIAVIENTAGQGSNLGYRFEHLAEIIDQVEDKNRVGVCLDT
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
CHLFSAGYDISSLESCEQTFSEFERTVGFQYLRGMHLNGSKTPLGSRVDRHHTLREGTIG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCH
TDFCKFIMQDDRFDNIPLILETIQPEIWTEEIKFLRTLAK
HHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYIGAHVSASGGVENAVLRAVEIGANAFALFTKNQRQWKALALKADTIEKFKRFCKAHQ
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
FSPEHILPHDSYLINLGNPEAENLAKSREAFIDEMERANQLGLKLLNFHPSAHLNKISES
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHHHCCHH
ECLARIAESINIAVDKVPNVIAVIENTAGQGSNLGYRFEHLAEIIDQVEDKNRVGVCLDT
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
CHLFSAGYDISSLESCEQTFSEFERTVGFQYLRGMHLNGSKTPLGSRVDRHHTLREGTIG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCH
TDFCKFIMQDDRFDNIPLILETIQPEIWTEEIKFLRTLAK
HHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA