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The map label for this gene is glgX [H]

Identifier: 126207831

GI number: 126207831

Start: 385723

End: 387708

Strand: Direct

Name: glgX [H]

Synonym: APL_0347

Alternate gene names: 126207831

Gene position: 385723-387708 (Clockwise)

Preceding gene: 126207830

Following gene: 126207832

Centisome position: 16.96

GC content: 42.45

Gene sequence:

>1986_bases
ATGAAAGTGACAGCGGGAAGTCCTTTTCCATTAGGTGCGCAAGCGGTCGAAAACGGCACGAATTTTGCAATTTTTTCAGA
AAATGCGACCGCTATCGAACTCTGTTTATTTAAGCAATCGCAAGAACAACGTTTTGCGATGCGACGTACCGGTGATGTAT
GGCATTTATTCGTTGAAAATATCGGAGAAGGCATCGAATACGGTTTCCGTGTCGCAGGGATTACCGATAAGCAAAAAGGC
GCGTTATTTAATCCGCAAAAGTTATTACTTGATCCTTATGCGAAACGTATCGTCGGTACGGTATCGGCGCATACGGCGGA
TGAAATTAAGTATTTTCAATGGGATGATAAACGTGATAACGCTCATTTAGCGCCTAAATCGGTAGTGGTTGCGCCGGATA
ATTTCGATTGGGAAGATGACGAACGCCCGCATTACCCGTGGAGAGAAACCATTATTTACGAGCTTCATGTAAAGGGTTTC
AGTCGGTTAAATCAACAGATTCCCGAACCGTTACGCGGGACTTATGCAGGTCTTGCACATCCGGCTTCTATTGCGTATCT
GAAGCGTTTAGGCGTCACGACCATAGAATTGCAACCGGTCAGCCATCATATTGATGAAGTGCACTTGCAGAAAATGGGGA
AAACCAATTATTGGGGATACAATGTTATCGGACATTCTGCAGTTGAGCCGGTGCTTGCTTGGGATCAAGAAGACCCGATT
ACCGAATTTAAATATTTGGTTAAAACATTACATAAACACGGTTTTGAAGTCATTTTAGATTTGGTTTATAACCATACCGC
AGAGGGCGGTACGGACAGTTTAACGCTATGTCAAAGAGGCATTGATAACCAAAATTATTATTGGCTGAATCAGCAAGGGG
AATATGAGAATTGGGCGGGATGCGGTAATGCGTTAAATTTAAGCCATCCGAGAGTGTGCCAGTGGGCGATAGACAGCTTG
GTTTACTGGGTAAGTGAATGCCATGTCGATGGATTCCGTTTCGATTTGGCAACCACATTAGGACGAACCCCCGAATTTGA
TCAATTATCGCCGTTTTTTGAAAAAATTAAGCAAACGCCTGAATTAGAACATATTAAATTAATCGCCGAGCCTTGGGATA
TTGGTCCGAACGGTTATCAAGTCGGCAGATTTCCGATTCAGTTTGCGGAATGGAATGATAAATACCGTACCACGATGCGA
GAATTCTTTTTAGGCGAGAGTGGCAATCTAGGCGAGTTTGCTCGTCGTTTAGCCGGTAGCGATGACCTTTATTGGCAATA
TTCTCCGGCAAAAAGTATCAACTATTTTGCTTCACACGACGGTTTTACTCTACAAGATCTCGTGAGTTATAACAAAAAGC
ATAATGAAGCAAACGGTGAAAATAATCGTGACGGTGAAAACACTAATCATAGTAATAACCACGGTGTTGAAGGCGAAACC
GATAACGAATTTGTAAATATTTTGCGAGATCAGACCGCTTGTAGCTTATTGGCGACCTTATTTCTCTCAAACGGCGTACC
TATGCTACGTGCCGGTGATGAGTTGGGGCATTCGCAAAAGGGTAATAACAACGCTTATTGCCAAGATAATGAGATAGCTT
GGATTGACTGGCAAAATGCAAATGAATCAAGAATTGAGTATGTAGCAAAATGGATAGCCTTACGTAAACGTATTGCCTTA
CTCTCTCACCAAAACCACTGGTGGACGGAGAATGACGTTCAATGGTTTTCATCGCAAGGGAAAGTGATGGAGATTGAAGA
TTGGCATAATACGGAGTCCAAAGCGTTACAAATTCTACTGCAAGATCATTGGCTGATCTTAATAAATGCCAAAAAAACCA
CCCAGCAATTTTTCCTACCGACAGGTAAATGGCATATTTGTCTCGGTAAGAATATTGCGACTTTGATAGCGAATACGCAA
TCGGTTGCTCTTCAGTTCGAACAGATGGGAGTGTGCGTGCTTCAAAGAAAATTAAGTTCATATTAA

Upstream 100 bases:

>100_bases
GGAAGTTGCGATGCAAAAAGCATTAAAACCCACTAAAAAAACGGTAAGCGTAAAAGCTAAAGCACATAAAAAAGCTCATA
AAAATAAGAAGTAACAAAAT

Downstream 100 bases:

>100_bases
TACCGGAGAAATCACATGCTTACACAAGAAAAAAACGACCTAAATAAATCTGGTCTCGATATTACTAACGATACGTTAGT
ACTTATTTTAGCGGGTGGTC

Product: glycogen operon protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MKVTAGSPFPLGAQAVENGTNFAIFSENATAIELCLFKQSQEQRFAMRRTGDVWHLFVENIGEGIEYGFRVAGITDKQKG
ALFNPQKLLLDPYAKRIVGTVSAHTADEIKYFQWDDKRDNAHLAPKSVVVAPDNFDWEDDERPHYPWRETIIYELHVKGF
SRLNQQIPEPLRGTYAGLAHPASIAYLKRLGVTTIELQPVSHHIDEVHLQKMGKTNYWGYNVIGHSAVEPVLAWDQEDPI
TEFKYLVKTLHKHGFEVILDLVYNHTAEGGTDSLTLCQRGIDNQNYYWLNQQGEYENWAGCGNALNLSHPRVCQWAIDSL
VYWVSECHVDGFRFDLATTLGRTPEFDQLSPFFEKIKQTPELEHIKLIAEPWDIGPNGYQVGRFPIQFAEWNDKYRTTMR
EFFLGESGNLGEFARRLAGSDDLYWQYSPAKSINYFASHDGFTLQDLVSYNKKHNEANGENNRDGENTNHSNNHGVEGET
DNEFVNILRDQTACSLLATLFLSNGVPMLRAGDELGHSQKGNNNAYCQDNEIAWIDWQNANESRIEYVAKWIALRKRIAL
LSHQNHWWTENDVQWFSSQGKVMEIEDWHNTESKALQILLQDHWLILINAKKTTQQFFLPTGKWHICLGKNIATLIANTQ
SVALQFEQMGVCVLQRKLSSY

Sequences:

>Translated_661_residues
MKVTAGSPFPLGAQAVENGTNFAIFSENATAIELCLFKQSQEQRFAMRRTGDVWHLFVENIGEGIEYGFRVAGITDKQKG
ALFNPQKLLLDPYAKRIVGTVSAHTADEIKYFQWDDKRDNAHLAPKSVVVAPDNFDWEDDERPHYPWRETIIYELHVKGF
SRLNQQIPEPLRGTYAGLAHPASIAYLKRLGVTTIELQPVSHHIDEVHLQKMGKTNYWGYNVIGHSAVEPVLAWDQEDPI
TEFKYLVKTLHKHGFEVILDLVYNHTAEGGTDSLTLCQRGIDNQNYYWLNQQGEYENWAGCGNALNLSHPRVCQWAIDSL
VYWVSECHVDGFRFDLATTLGRTPEFDQLSPFFEKIKQTPELEHIKLIAEPWDIGPNGYQVGRFPIQFAEWNDKYRTTMR
EFFLGESGNLGEFARRLAGSDDLYWQYSPAKSINYFASHDGFTLQDLVSYNKKHNEANGENNRDGENTNHSNNHGVEGET
DNEFVNILRDQTACSLLATLFLSNGVPMLRAGDELGHSQKGNNNAYCQDNEIAWIDWQNANESRIEYVAKWIALRKRIAL
LSHQNHWWTENDVQWFSSQGKVMEIEDWHNTESKALQILLQDHWLILINAKKTTQQFFLPTGKWHICLGKNIATLIANTQ
SVALQFEQMGVCVLQRKLSSY
>Mature_661_residues
MKVTAGSPFPLGAQAVENGTNFAIFSENATAIELCLFKQSQEQRFAMRRTGDVWHLFVENIGEGIEYGFRVAGITDKQKG
ALFNPQKLLLDPYAKRIVGTVSAHTADEIKYFQWDDKRDNAHLAPKSVVVAPDNFDWEDDERPHYPWRETIIYELHVKGF
SRLNQQIPEPLRGTYAGLAHPASIAYLKRLGVTTIELQPVSHHIDEVHLQKMGKTNYWGYNVIGHSAVEPVLAWDQEDPI
TEFKYLVKTLHKHGFEVILDLVYNHTAEGGTDSLTLCQRGIDNQNYYWLNQQGEYENWAGCGNALNLSHPRVCQWAIDSL
VYWVSECHVDGFRFDLATTLGRTPEFDQLSPFFEKIKQTPELEHIKLIAEPWDIGPNGYQVGRFPIQFAEWNDKYRTTMR
EFFLGESGNLGEFARRLAGSDDLYWQYSPAKSINYFASHDGFTLQDLVSYNKKHNEANGENNRDGENTNHSNNHGVEGET
DNEFVNILRDQTACSLLATLFLSNGVPMLRAGDELGHSQKGNNNAYCQDNEIAWIDWQNANESRIEYVAKWIALRKRIAL
LSHQNHWWTENDVQWFSSQGKVMEIEDWHNTESKALQILLQDHWLILINAKKTTQQFFLPTGKWHICLGKNIATLIANTQ
SVALQFEQMGVCVLQRKLSSY

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=664, Percent_Identity=48.0421686746988, Blast_Score=611, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75736; Mature: 75736

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTAGSPFPLGAQAVENGTNFAIFSENATAIELCLFKQSQEQRFAMRRTGDVWHLFVEN
CCCCCCCCCCCCHHHHCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHHHCCCHHHHHHHH
IGEGIEYGFRVAGITDKQKGALFNPQKLLLDPYAKRIVGTVSAHTADEIKYFQWDDKRDN
HCCHHHCCEEEECCCCCCCCCCCCCHHHHHCHHHHHHHHHHHCCCHHHEEEEECCCCCCC
AHLAPKSVVVAPDNFDWEDDERPHYPWRETIIYELHVKGFSRLNQQIPEPLRGTYAGLAH
CCCCCCEEEECCCCCCCCCCCCCCCCHHHEEEEEEEHHHHHHHHHHCCHHHHHHHHCCCC
PASIAYLKRLGVTTIELQPVSHHIDEVHLQKMGKTNYWGYNVIGHSAVEPVLAWDQEDPI
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEECCCHHHHCHHHCCCCCCCH
TEFKYLVKTLHKHGFEVILDLVYNHTAEGGTDSLTLCQRGIDNQNYYWLNQQGEYENWAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHC
CGNALNLSHPRVCQWAIDSLVYWVSECHVDGFRFDLATTLGRTPEFDQLSPFFEKIKQTP
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHCCC
ELEHIKLIAEPWDIGPNGYQVGRFPIQFAEWNDKYRTTMREFFLGESGNLGEFARRLAGS
CCCEEEEEECCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCC
DDLYWQYSPAKSINYFASHDGFTLQDLVSYNKKHNEANGENNRDGENTNHSNNHGVEGET
CCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
DNEFVNILRDQTACSLLATLFLSNGVPMLRAGDELGHSQKGNNNAYCQDNEIAWIDWQNA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCEECCCEEEEEECCC
NESRIEYVAKWIALRKRIALLSHQNHWWTENDVQWFSSQGKVMEIEDWHNTESKALQILL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEECCCCCCHHHHHHHHE
QDHWLILINAKKTTQQFFLPTGKWHICLGKNIATLIANTQSVALQFEQMGVCVLQRKLSS
ECCEEEEEECCCCCHHEECCCCCEEEEECCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHC
Y
C
>Mature Secondary Structure
MKVTAGSPFPLGAQAVENGTNFAIFSENATAIELCLFKQSQEQRFAMRRTGDVWHLFVEN
CCCCCCCCCCCCHHHHCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHHHCCCHHHHHHHH
IGEGIEYGFRVAGITDKQKGALFNPQKLLLDPYAKRIVGTVSAHTADEIKYFQWDDKRDN
HCCHHHCCEEEECCCCCCCCCCCCCHHHHHCHHHHHHHHHHHCCCHHHEEEEECCCCCCC
AHLAPKSVVVAPDNFDWEDDERPHYPWRETIIYELHVKGFSRLNQQIPEPLRGTYAGLAH
CCCCCCEEEECCCCCCCCCCCCCCCCHHHEEEEEEEHHHHHHHHHHCCHHHHHHHHCCCC
PASIAYLKRLGVTTIELQPVSHHIDEVHLQKMGKTNYWGYNVIGHSAVEPVLAWDQEDPI
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEECCCHHHHCHHHCCCCCCCH
TEFKYLVKTLHKHGFEVILDLVYNHTAEGGTDSLTLCQRGIDNQNYYWLNQQGEYENWAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHC
CGNALNLSHPRVCQWAIDSLVYWVSECHVDGFRFDLATTLGRTPEFDQLSPFFEKIKQTP
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHCCC
ELEHIKLIAEPWDIGPNGYQVGRFPIQFAEWNDKYRTTMREFFLGESGNLGEFARRLAGS
CCCEEEEEECCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCC
DDLYWQYSPAKSINYFASHDGFTLQDLVSYNKKHNEANGENNRDGENTNHSNNHGVEGET
CCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
DNEFVNILRDQTACSLLATLFLSNGVPMLRAGDELGHSQKGNNNAYCQDNEIAWIDWQNA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCEECCCEEEEEECCC
NESRIEYVAKWIALRKRIALLSHQNHWWTENDVQWFSSQGKVMEIEDWHNTESKALQILL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEECCCCCCHHHHHHHHE
QDHWLILINAKKTTQQFFLPTGKWHICLGKNIATLIANTQSVALQFEQMGVCVLQRKLSS
ECCEEEEEECCCCCHHEECCCCCEEEEECCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]