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The map label for this gene is glgB

Identifier: 126207830

GI number: 126207830

Start: 383383

End: 385716

Strand: Direct

Name: glgB

Synonym: APL_0346

Alternate gene names: 126207830

Gene position: 383383-385716 (Clockwise)

Preceding gene: 126207813

Following gene: 126207831

Centisome position: 16.86

GC content: 43.23

Gene sequence:

>2334_bases
ATGAAAACATATACGTATTCTAAACAAGATTTATCTTTTATTGAGCAACTTTCTCAAGCGTATTGTAAAGATCCTTTCTC
TTATCTCGGCTTACATCAAGCGGGTGATGTGAGCGTAATTCGTGTTTTTCTACCGGAAGCAACGACGGTTAAAATTCTGA
GTGCAGTCGGTCAAATACTTTCCGAAGCTTTGAAAATAGACGACTCAGGCTTGTTTGTAGCGCAATTGGCGCAGCAGTAT
TCTTCCTTAAATTATCGTTTAAGAGTCGGTTATTCTTTAGCGGAAATCGACCTTGAAGATCCCTATCGTTTTACTTCTTC
GCTTCTACCGATGGATAACTGGTTATTGGCGGAAGGAACTCACCTTCGTCCATACGAAATCTTAGGTGCGCATTTGAAAA
CGCAAGAAGGCGTTTCCGGCGTACATTTTTCCGTTTGGGCGCCGAATGCCCGTCGTGTATCGGTTGTCGGCGATTTCAAC
TATTGGGACGGGCGAGTAAACCCGATGCGTTTTCATGCGGAAAGCGGCATTTGGGATATTTTCCTACCTAATGTTGAGAA
AGGTGCATTATATAAATTCGAAATTTTAGATAGCAACGGTAACATTCGTTTGAAATCCGACCCTTATGCGTTCGCATCGC
AATTCAGACCGGATACGGCATCCGTAGTAACGGGTTTACCGGAAAAAATAGAAGTGGATGCGAAACTACGTCATGCGAAT
GATCCCGACCAGCCGATTTCAATTTATGAAGTGCATCTCGGTTCGTGGCGTCGTCACCTTGAAAATAATTATTGGCTGAA
TTATGAAGAAATTGCCAACGAATTAATTCCTTATGTTAAAGATATGGGGTTCACTCATATCGAATTGTTACCGATTACCG
AATATCCGTTCGACGGTTCTTGGGGTTATCAACCGACCGGACTTTATTCTCCGACCAGCCGATTCGGTTCGCCGGAGGAT
TTACGTACCTTGATTCGTAAAGCGCATGAAGCGGGTATTAATGTGATTCTTGACTGGGTAGTCGGGCATTTCCCGACCGA
TTCGCACGGTTTAACCGAGTTTGACGGCTCGCATTTATATGAACATCAAGATCCGCGAGAAGGTTATCATCAAGATTGGA
ATACCTTAATTTTTAATTACGGTCGTCACGAGGTGTTTAATTATCTTTCCAGTAACGCCTTATATTGGACGGAACGATTC
GGTATCGACGGTTTACGTGTCGATGCGGTTTCTTCCATGATTTACCGCGATTATTCTCGTAAAGACGGCGAGTGGATTCC
GAATCAATACGGCGGACGAGAAAATTTAGAAGCGCTTGATTTCCTTCGTAGAACCAATCGTATGTTGAAAAAAGAAGGAC
ACGGAGCGGTTGTGATTGCGGAGGAATCCACTTCATTTGCCGGTATTACGCATAGTCCGGAGGAAAACGGGGTCGGTTTC
GACTATAAATGGAATATGGGCTGGATGAACGATACCTTACGTTATATGTCGCTCGATCCGATTTATCGTCAATATCATCA
CGATTGGATGACCTTCGGGATGATGTATCAATATAGCGAAAAATTCGTATTACCGCTTTCACACGATGAAGTGGTGCATG
GCAAATGTTCCATTCTCGGAAAAATGTCCGGCGATTGTTGGCAAAAATTTGCGAACTTAAGAGCCTATTACGGTTATATG
TGGGGCTATCCGGGTAAAAAATTACTGTTTATGGGTAATGAATTCGCCCAAGGTCGTGAATGGAATTACAACGAAAGCCT
CGATTGGTTCCTGTTAGGCGAACAAGGCGGCGGTTGGCATAAAGGCGTGCTGAATTGGGTGAGAGATTTGAACCGTACTT
ACCAAAAATATCCGGCGCTTTATCAATTGGATTACGATCCTGCCGGTTTTGAATGGTTAGTAGTCGATGACTGGCAACAA
TCGGTATTTGCTTTTGAACGTAAAGCCAAAAACGGCGAGAGTGTGATCGTAGTCAGTAACTTTACGCCGGTCGTTCGCCA
TAATTATCGTATAGGCGTGCGTCAAGACGGCACTTATACTGAAATCCTCAATTCGGATGCGGCTTACTATGAAGGAAGTA
ACGTAGGCAATTACGGCGAGATTGAATGCGAAGCAATCGAATCGCACGGTAAACCGTTCTCTATTGAACTTTCGATTCCG
CCGTTATCAACCATTTTTATTGCTTGTCAGCCTAAGCCTAAAGAAGCGGTTGAAGCCGAACAAGATATTGTGAAAATGGC
GGAAGTTGCGATGCAAAAAGCATTAAAACCCACTAAAAAAACGGTAAGCGTAAAAGCTAAAGCACATAAAAAAGCTCATA
AAAATAAGAAGTAA

Upstream 100 bases:

>100_bases
CAATGCTTCGCCGAAGACCATTAGAAAATGTTATGATGCAAACGTTTTCGTAGTCAGGAAGAAAATAGTCAAAAGTGTGA
AAAGCGAGGATGGTTATAGC

Downstream 100 bases:

>100_bases
CAAAATATGAAAGTGACAGCGGGAAGTCCTTTTCCATTAGGTGCGCAAGCGGTCGAAAACGGCACGAATTTTGCAATTTT
TTCAGAAAATGCGACCGCTA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MKTYTYSKQDLSFIEQLSQAYCKDPFSYLGLHQAGDVSVIRVFLPEATTVKILSAVGQILSEALKIDDSGLFVAQLAQQY
SSLNYRLRVGYSLAEIDLEDPYRFTSSLLPMDNWLLAEGTHLRPYEILGAHLKTQEGVSGVHFSVWAPNARRVSVVGDFN
YWDGRVNPMRFHAESGIWDIFLPNVEKGALYKFEILDSNGNIRLKSDPYAFASQFRPDTASVVTGLPEKIEVDAKLRHAN
DPDQPISIYEVHLGSWRRHLENNYWLNYEEIANELIPYVKDMGFTHIELLPITEYPFDGSWGYQPTGLYSPTSRFGSPED
LRTLIRKAHEAGINVILDWVVGHFPTDSHGLTEFDGSHLYEHQDPREGYHQDWNTLIFNYGRHEVFNYLSSNALYWTERF
GIDGLRVDAVSSMIYRDYSRKDGEWIPNQYGGRENLEALDFLRRTNRMLKKEGHGAVVIAEESTSFAGITHSPEENGVGF
DYKWNMGWMNDTLRYMSLDPIYRQYHHDWMTFGMMYQYSEKFVLPLSHDEVVHGKCSILGKMSGDCWQKFANLRAYYGYM
WGYPGKKLLFMGNEFAQGREWNYNESLDWFLLGEQGGGWHKGVLNWVRDLNRTYQKYPALYQLDYDPAGFEWLVVDDWQQ
SVFAFERKAKNGESVIVVSNFTPVVRHNYRIGVRQDGTYTEILNSDAAYYEGSNVGNYGEIECEAIESHGKPFSIELSIP
PLSTIFIACQPKPKEAVEAEQDIVKMAEVAMQKALKPTKKTVSVKAKAHKKAHKNKK

Sequences:

>Translated_777_residues
MKTYTYSKQDLSFIEQLSQAYCKDPFSYLGLHQAGDVSVIRVFLPEATTVKILSAVGQILSEALKIDDSGLFVAQLAQQY
SSLNYRLRVGYSLAEIDLEDPYRFTSSLLPMDNWLLAEGTHLRPYEILGAHLKTQEGVSGVHFSVWAPNARRVSVVGDFN
YWDGRVNPMRFHAESGIWDIFLPNVEKGALYKFEILDSNGNIRLKSDPYAFASQFRPDTASVVTGLPEKIEVDAKLRHAN
DPDQPISIYEVHLGSWRRHLENNYWLNYEEIANELIPYVKDMGFTHIELLPITEYPFDGSWGYQPTGLYSPTSRFGSPED
LRTLIRKAHEAGINVILDWVVGHFPTDSHGLTEFDGSHLYEHQDPREGYHQDWNTLIFNYGRHEVFNYLSSNALYWTERF
GIDGLRVDAVSSMIYRDYSRKDGEWIPNQYGGRENLEALDFLRRTNRMLKKEGHGAVVIAEESTSFAGITHSPEENGVGF
DYKWNMGWMNDTLRYMSLDPIYRQYHHDWMTFGMMYQYSEKFVLPLSHDEVVHGKCSILGKMSGDCWQKFANLRAYYGYM
WGYPGKKLLFMGNEFAQGREWNYNESLDWFLLGEQGGGWHKGVLNWVRDLNRTYQKYPALYQLDYDPAGFEWLVVDDWQQ
SVFAFERKAKNGESVIVVSNFTPVVRHNYRIGVRQDGTYTEILNSDAAYYEGSNVGNYGEIECEAIESHGKPFSIELSIP
PLSTIFIACQPKPKEAVEAEQDIVKMAEVAMQKALKPTKKTVSVKAKAHKKAHKNKK
>Mature_777_residues
MKTYTYSKQDLSFIEQLSQAYCKDPFSYLGLHQAGDVSVIRVFLPEATTVKILSAVGQILSEALKIDDSGLFVAQLAQQY
SSLNYRLRVGYSLAEIDLEDPYRFTSSLLPMDNWLLAEGTHLRPYEILGAHLKTQEGVSGVHFSVWAPNARRVSVVGDFN
YWDGRVNPMRFHAESGIWDIFLPNVEKGALYKFEILDSNGNIRLKSDPYAFASQFRPDTASVVTGLPEKIEVDAKLRHAN
DPDQPISIYEVHLGSWRRHLENNYWLNYEEIANELIPYVKDMGFTHIELLPITEYPFDGSWGYQPTGLYSPTSRFGSPED
LRTLIRKAHEAGINVILDWVVGHFPTDSHGLTEFDGSHLYEHQDPREGYHQDWNTLIFNYGRHEVFNYLSSNALYWTERF
GIDGLRVDAVSSMIYRDYSRKDGEWIPNQYGGRENLEALDFLRRTNRMLKKEGHGAVVIAEESTSFAGITHSPEENGVGF
DYKWNMGWMNDTLRYMSLDPIYRQYHHDWMTFGMMYQYSEKFVLPLSHDEVVHGKCSILGKMSGDCWQKFANLRAYYGYM
WGYPGKKLLFMGNEFAQGREWNYNESLDWFLLGEQGGGWHKGVLNWVRDLNRTYQKYPALYQLDYDPAGFEWLVVDDWQQ
SVFAFERKAKNGESVIVVSNFTPVVRHNYRIGVRQDGTYTEILNSDAAYYEGSNVGNYGEIECEAIESHGKPFSIELSIP
PLSTIFIACQPKPKEAVEAEQDIVKMAEVAMQKALKPTKKTVSVKAKAHKKAHKNKK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=646, Percent_Identity=27.2445820433437, Blast_Score=185, Evalue=1e-46,
Organism=Escherichia coli, GI1789839, Length=724, Percent_Identity=60.9116022099447, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI2367229, Length=351, Percent_Identity=26.7806267806268, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554896, Length=624, Percent_Identity=28.3653846153846, Blast_Score=183, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32564391, Length=587, Percent_Identity=28.1090289608177, Blast_Score=160, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320826, Length=697, Percent_Identity=26.5423242467719, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI28573410, Length=606, Percent_Identity=27.7227722772277, Blast_Score=176, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_ACTP2 (A3MZ64)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053055.1
- ProteinModelPortal:   A3MZ64
- SMR:   A3MZ64
- STRING:   A3MZ64
- GeneID:   4849471
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0346
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   A3MZ64
- ProtClustDB:   PRK05402
- BioCyc:   APLE416269:APL_0346-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 89300; Mature: 89300

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: ACT_SITE 303-303 ACT_SITE 338-338 ACT_SITE 343-343 ACT_SITE 406-406 ACT_SITE 408-408 ACT_SITE 461-461 ACT_SITE 528-528 ACT_SITE 529-529

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYTYSKQDLSFIEQLSQAYCKDPFSYLGLHQAGDVSVIRVFLPEATTVKILSAVGQIL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
SEALKIDDSGLFVAQLAQQYSSLNYRLRVGYSLAEIDLEDPYRFTSSLLPMDNWLLAEGT
HHHHCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHCCCCCCEEEECCC
HLRPYEILGAHLKTQEGVSGVHFSVWAPNARRVSVVGDFNYWDGRVNPMRFHAESGIWDI
CCCHHHHHCCHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEE
FLPNVEKGALYKFEILDSNGNIRLKSDPYAFASQFRPDTASVVTGLPEKIEVDAKLRHAN
ECCCCCCCCEEEEEEECCCCCEEECCCCHHHHHHCCCCHHHHHHCCCCCEEECEEEECCC
DPDQPISIYEVHLGSWRRHLENNYWLNYEEIANELIPYVKDMGFTHIELLPITEYPFDGS
CCCCCEEEEEEEHHHHHHHHCCCCEECHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
WGYQPTGLYSPTSRFGSPEDLRTLIRKAHEAGINVILDWVVGHFPTDSHGLTEFDGSHLY
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCHHEEEEHHHHCCCCCCCCCCCCCCCCCC
EHQDPREGYHQDWNTLIFNYGRHEVFNYLSSNALYWTERFGIDGLRVDAVSSMIYRDYSR
CCCCCCHHHHCCCCCEEEECCHHHHHHHHHCCEEEEEHHCCCCCEEHHHHHHHHHHHCCC
KDGEWIPNQYGGRENLEALDFLRRTNRMLKKEGHGAVVIAEESTSFAGITHSPEENGVGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCE
DYKWNMGWMNDTLRYMSLDPIYRQYHHDWMTFGMMYQYSEKFVLPLSHDEVVHGKCSILG
EEEECCCCCCCCHHHEECCHHHHHHHHHHHHHHHHEECCCCEEEECCCCCEECCCEEEEE
KMSGDCWQKFANLRAYYGYMWGYPGKKLLFMGNEFAQGREWNYNESLDWFLLGEQGGGWH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEECCCCCHH
KGVLNWVRDLNRTYQKYPALYQLDYDPAGFEWLVVDDWQQSVFAFERKAKNGESVIVVSN
HHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECHHHHHHHHHHHCCCCCEEEEEEC
FTPVVRHNYRIGVRQDGTYTEILNSDAAYYEGSNVGNYGEIECEAIESHGKPFSIELSIP
CCHHHHCCEEEEEECCCCHHHHHCCCCCEECCCCCCCCCCEEEEEHHCCCCEEEEEEECC
PLSTIFIACQPKPKEAVEAEQDIVKMAEVAMQKALKPTKKTVSVKAKAHKKAHKNKK
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHCCCC
>Mature Secondary Structure
MKTYTYSKQDLSFIEQLSQAYCKDPFSYLGLHQAGDVSVIRVFLPEATTVKILSAVGQIL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
SEALKIDDSGLFVAQLAQQYSSLNYRLRVGYSLAEIDLEDPYRFTSSLLPMDNWLLAEGT
HHHHCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHCCCCCCEEEECCC
HLRPYEILGAHLKTQEGVSGVHFSVWAPNARRVSVVGDFNYWDGRVNPMRFHAESGIWDI
CCCHHHHHCCHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEE
FLPNVEKGALYKFEILDSNGNIRLKSDPYAFASQFRPDTASVVTGLPEKIEVDAKLRHAN
ECCCCCCCCEEEEEEECCCCCEEECCCCHHHHHHCCCCHHHHHHCCCCCEEECEEEECCC
DPDQPISIYEVHLGSWRRHLENNYWLNYEEIANELIPYVKDMGFTHIELLPITEYPFDGS
CCCCCEEEEEEEHHHHHHHHCCCCEECHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
WGYQPTGLYSPTSRFGSPEDLRTLIRKAHEAGINVILDWVVGHFPTDSHGLTEFDGSHLY
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCHHEEEEHHHHCCCCCCCCCCCCCCCCCC
EHQDPREGYHQDWNTLIFNYGRHEVFNYLSSNALYWTERFGIDGLRVDAVSSMIYRDYSR
CCCCCCHHHHCCCCCEEEECCHHHHHHHHHCCEEEEEHHCCCCCEEHHHHHHHHHHHCCC
KDGEWIPNQYGGRENLEALDFLRRTNRMLKKEGHGAVVIAEESTSFAGITHSPEENGVGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCE
DYKWNMGWMNDTLRYMSLDPIYRQYHHDWMTFGMMYQYSEKFVLPLSHDEVVHGKCSILG
EEEECCCCCCCCHHHEECCHHHHHHHHHHHHHHHHEECCCCEEEECCCCCEECCCEEEEE
KMSGDCWQKFANLRAYYGYMWGYPGKKLLFMGNEFAQGREWNYNESLDWFLLGEQGGGWH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEECCCCCHH
KGVLNWVRDLNRTYQKYPALYQLDYDPAGFEWLVVDDWQQSVFAFERKAKNGESVIVVSN
HHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECHHHHHHHHHHHCCCCCEEEEEEC
FTPVVRHNYRIGVRQDGTYTEILNSDAAYYEGSNVGNYGEIECEAIESHGKPFSIELSIP
CCHHHHCCEEEEEECCCCHHHHHCCCCCEECCCCCCCCCCEEEEEHHCCCCEEEEEEECC
PLSTIFIACQPKPKEAVEAEQDIVKMAEVAMQKALKPTKKTVSVKAKAHKKAHKNKK
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA