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The map label for this gene is fruB [H]

Identifier: 126207829

GI number: 126207829

Start: 381614

End: 383122

Strand: Reverse

Name: fruB [H]

Synonym: APL_0345

Alternate gene names: 126207829

Gene position: 383122-381614 (Counterclockwise)

Preceding gene: 126207835

Following gene: 126207828

Centisome position: 16.84

GC content: 45.53

Gene sequence:

>1509_bases
ATGCTAAATTTATCGGCAAAAAATATCTGCTTAAATAGCTCGGTAAACAATAAAGACGAAGCGATTAAACTTGTTGCCGC
CGGCTTAACGGCAAACGGTAATGTTGCGGAAGGTTATGAAGCCGGAATGCTTGCGCGCGAAACGCAAACCTCAACTTTTC
TAGGTAACGGTATTGCAATTCCGCACGGCACATTGGATACCCGCCACCTTGTACAAAATACCGGTGTACAAATTTATCAA
TTCCCGCAAGGTGTGGAGTGGGGTGAAGGCAATAAAGCCTATGTTGTGATTGGTATTGCGGCAAAATCCGACGAGCATTT
AACACTATTGCGTCAATTAACCGGCGTATTAAGCGACGAAGCGGCGGCGGAATTACTCGCAACTACGCAAGATACGGACG
AATTTATCAGCGTGCTGAGCGGTAAAAAGTCCTTACCGAGTTTGACCGATACCCTCATTTCATTAAATATCGATTCTCCG
AGCCTTATTACGCTTTCTGCGATTAATGCCGCAAAATTGCAAGAAAACGGTTATGTCAATCAGGCATTTATCAGCGAAGC
GATTTCAAATCAGCCGCTTAATTTAGGCAACAACATTTATCTTACCGATGCAGCTCAAGGCAATCAATCAAACGGTATCG
CCGTTGCACGTAACATGTCAGGTCAAACGGTCATTACCGTTGCGGCAACAGACGAGCATCTACAAAGCCATCTGGCTTAC
TTATTAAACGGCGATATTCGTAGCCAATTCTCAACCGCTTCCGCAGAACAAATTATTGCGCTTTTTAACGGTGAAAGCAT
TGTAAATTCCGCTCAAAATACCACCGCTTGCGTAAATCTTGCAACAGGTCAGGTTATCGGTACTTTCACCGTACGTAACG
ACAATGGTTTACACGCTCGCCCAAGCGCAGTATTAGTGCAAACCTTAAAACCGTTTGCGGCAAAAGTAACCGTTGAAAAC
CTAGATCGAGGTACTGCACCGGCAAATGCGAAAAGTACGATGAAAGTGGTGGCGTTAGGTGCAAGTCAAGCACACCGCTT
ACGTTTTGTGGCAGAAGGTGAAGACGCTCAACAAGCGATCGAAGCATTAGCAAAAGCATTCGTAGAAGGTTTAGGCGAAA
GCGTTTCATTCGTGCCTGCGGTTGAAGATACGATTGAAGGTGCGGCACAACCGCAAGCGGTTGAATCTGCAAAAAATTTT
GCAAATCCGACCGCTTCCGAGCCTACGGTGGAAGGTCAAGTGGAAGGCACGTTCGTGATTCAAAACGAACACGGTTTACA
CGCCCGCCCAAGTGCAGTGTTAGTGAATGAAGTAAAAAAATATAACGCGACTATCGTTGTACAAAACTTAGACCGTAATA
CTCAATTAGTCAGTGCGAAAAGCTTAATGAAAATTGTAGCACTTGGCGTCGTAAAAGGTCATCGCTTACATTTCGTAGCA
ACCGGTGACGACGCTCAAAAAGCGATTGACGGTATCGGCGAGGCAATTGCGGCAGGCTTAGGCGAGTAA

Upstream 100 bases:

>100_bases
CTGATATTTTTTTCGGCTTGCTAGAACGATTTAGCAGAATATAGGTTACAATATCGGACAATATTTTTATTTTTAAAATT
AAATCTGATAAGGAGTTACG

Downstream 100 bases:

>100_bases
TTGAGGAACAGACAATGACACAACAATTACGAATTGCAACCGTTACCTTAAATCCAGCGTTTGACTTGGTCGGCCGCCTA
CAACGTATTGAAGTGGGCGA

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Fructose PTS diphosphoryl transfer protein; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIIA-Fru; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MLNLSAKNICLNSSVNNKDEAIKLVAAGLTANGNVAEGYEAGMLARETQTSTFLGNGIAIPHGTLDTRHLVQNTGVQIYQ
FPQGVEWGEGNKAYVVIGIAAKSDEHLTLLRQLTGVLSDEAAAELLATTQDTDEFISVLSGKKSLPSLTDTLISLNIDSP
SLITLSAINAAKLQENGYVNQAFISEAISNQPLNLGNNIYLTDAAQGNQSNGIAVARNMSGQTVITVAATDEHLQSHLAY
LLNGDIRSQFSTASAEQIIALFNGESIVNSAQNTTACVNLATGQVIGTFTVRNDNGLHARPSAVLVQTLKPFAAKVTVEN
LDRGTAPANAKSTMKVVALGASQAHRLRFVAEGEDAQQAIEALAKAFVEGLGESVSFVPAVEDTIEGAAQPQAVESAKNF
ANPTASEPTVEGQVEGTFVIQNEHGLHARPSAVLVNEVKKYNATIVVQNLDRNTQLVSAKSLMKIVALGVVKGHRLHFVA
TGDDAQKAIDGIGEAIAAGLGE

Sequences:

>Translated_502_residues
MLNLSAKNICLNSSVNNKDEAIKLVAAGLTANGNVAEGYEAGMLARETQTSTFLGNGIAIPHGTLDTRHLVQNTGVQIYQ
FPQGVEWGEGNKAYVVIGIAAKSDEHLTLLRQLTGVLSDEAAAELLATTQDTDEFISVLSGKKSLPSLTDTLISLNIDSP
SLITLSAINAAKLQENGYVNQAFISEAISNQPLNLGNNIYLTDAAQGNQSNGIAVARNMSGQTVITVAATDEHLQSHLAY
LLNGDIRSQFSTASAEQIIALFNGESIVNSAQNTTACVNLATGQVIGTFTVRNDNGLHARPSAVLVQTLKPFAAKVTVEN
LDRGTAPANAKSTMKVVALGASQAHRLRFVAEGEDAQQAIEALAKAFVEGLGESVSFVPAVEDTIEGAAQPQAVESAKNF
ANPTASEPTVEGQVEGTFVIQNEHGLHARPSAVLVNEVKKYNATIVVQNLDRNTQLVSAKSLMKIVALGVVKGHRLHFVA
TGDDAQKAIDGIGEAIAAGLGE
>Mature_502_residues
MLNLSAKNICLNSSVNNKDEAIKLVAAGLTANGNVAEGYEAGMLARETQTSTFLGNGIAIPHGTLDTRHLVQNTGVQIYQ
FPQGVEWGEGNKAYVVIGIAAKSDEHLTLLRQLTGVLSDEAAAELLATTQDTDEFISVLSGKKSLPSLTDTLISLNIDSP
SLITLSAINAAKLQENGYVNQAFISEAISNQPLNLGNNIYLTDAAQGNQSNGIAVARNMSGQTVITVAATDEHLQSHLAY
LLNGDIRSQFSTASAEQIIALFNGESIVNSAQNTTACVNLATGQVIGTFTVRNDNGLHARPSAVLVQTLKPFAAKVTVEN
LDRGTAPANAKSTMKVVALGASQAHRLRFVAEGEDAQQAIEALAKAFVEGLGESVSFVPAVEDTIEGAAQPQAVESAKNF
ANPTASEPTVEGQVEGTFVIQNEHGLHARPSAVLVNEVKKYNATIVVQNLDRNTQLVSAKSLMKIVALGVVKGHRLHFVA
TGDDAQKAIDGIGEAIAAGLGE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=386, Percent_Identity=46.1139896373057, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=41.3793103448276, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016258
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 52680; Mature: 52680

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLSAKNICLNSSVNNKDEAIKLVAAGLTANGNVAEGYEAGMLARETQTSTFLGNGIAI
CCCCCCCCEEECCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCEEC
PHGTLDTRHLVQNTGVQIYQFPQGVEWGEGNKAYVVIGIAAKSDEHLTLLRQLTGVLSDE
CCCCCCHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCHH
AAAELLATTQDTDEFISVLSGKKSLPSLTDTLISLNIDSPSLITLSAINAAKLQENGYVN
HHHHHHHCCCCHHHHHHHHCCCCCCCCHHEEEEEEECCCCCEEEEEECCHHHHHCCCCCH
QAFISEAISNQPLNLGNNIYLTDAAQGNQSNGIAVARNMSGQTVITVAATDEHLQSHLAY
HHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECHHHHHHHHHH
LLNGDIRSQFSTASAEQIIALFNGESIVNSAQNTTACVNLATGQVIGTFTVRNDNGLHAR
HHCCHHHHHHCCCCHHHEEEEECCCHHHHCCCCCEEEEEEECCCEEEEEEEECCCCCCCC
PSAVLVQTLKPFAAKVTVENLDRGTAPANAKSTMKVVALGASQAHRLRFVAEGEDAQQAI
CHHHHHHHHCCHHHEEEHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHH
EALAKAFVEGLGESVSFVPAVEDTIEGAAQPQAVESAKNFANPTASEPTVEGQVEGTFVI
HHHHHHHHHHCCCCCEECCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
QNEHGLHARPSAVLVNEVKKYNATIVVQNLDRNTQLVSAKSLMKIVALGVVKGHRLHFVA
ECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TGDDAQKAIDGIGEAIAAGLGE
CCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNLSAKNICLNSSVNNKDEAIKLVAAGLTANGNVAEGYEAGMLARETQTSTFLGNGIAI
CCCCCCCCEEECCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCEEC
PHGTLDTRHLVQNTGVQIYQFPQGVEWGEGNKAYVVIGIAAKSDEHLTLLRQLTGVLSDE
CCCCCCHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCHH
AAAELLATTQDTDEFISVLSGKKSLPSLTDTLISLNIDSPSLITLSAINAAKLQENGYVN
HHHHHHHCCCCHHHHHHHHCCCCCCCCHHEEEEEEECCCCCEEEEEECCHHHHHCCCCCH
QAFISEAISNQPLNLGNNIYLTDAAQGNQSNGIAVARNMSGQTVITVAATDEHLQSHLAY
HHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECHHHHHHHHHH
LLNGDIRSQFSTASAEQIIALFNGESIVNSAQNTTACVNLATGQVIGTFTVRNDNGLHAR
HHCCHHHHHHCCCCHHHEEEEECCCHHHHCCCCCEEEEEEECCCEEEEEEEECCCCCCCC
PSAVLVQTLKPFAAKVTVENLDRGTAPANAKSTMKVVALGASQAHRLRFVAEGEDAQQAI
CHHHHHHHHCCHHHEEEHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHH
EALAKAFVEGLGESVSFVPAVEDTIEGAAQPQAVESAKNFANPTASEPTVEGQVEGTFVI
HHHHHHHHHHCCCCCEECCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
QNEHGLHARPSAVLVNEVKKYNATIVVQNLDRNTQLVSAKSLMKIVALGVVKGHRLHFVA
ECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TGDDAQKAIDGIGEAIAAGLGE
CCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 8763608 [H]