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The map label for this gene is fruA [H]

Identifier: 126207827

GI number: 126207827

Start: 378909

End: 380570

Strand: Reverse

Name: fruA [H]

Synonym: APL_0343

Alternate gene names: 126207827

Gene position: 380570-378909 (Counterclockwise)

Preceding gene: 126207828

Following gene: 126207826

Centisome position: 16.73

GC content: 45.97

Gene sequence:

>1662_bases
ATGAATATTTCCTTTGTATTCCCTCAATCATTAGGTAAAGCCCGTGCTTTCCTCGTGAATGAAGTATTATCGGCGGCAGC
CAAGCAACAAGGCCATCAAGTTGTAAACGCTGAACAAGCTGATTTTGTTGTGTTATTTAACGATGAAATTCCGACTATTG
CTGCCGGCAAACAAGGTGCGGTGGTGAATTTAGATCAAGCTTTCTCTCAACCGGAAGCAACGCTTAAACAAGCGGTCGAA
AATGCAAAAACTTTTGCAAATGCAACCGTTTCTAGCGCATCAAATTCAGGTATGAAAAACATCGTAGCGGTAACCGCTTG
CCCGACCGGTGTGGCACATACTTTTATGTCTGCGGAAGCTATCGAAAACTACGCCAAAGCACAAGGTTGGAACGTTAAAG
TTGAAACCCGCGGTCAGGTTGGTGCGGGTAACCCGATTTCTGCGGAAGAAATTGCAGCGGCGGACTTAGTCTTCGTGGCG
GCGGATATTGACGTGGATTTAGACAAATTCAAAGGTAAACCGATGTACCGTACCTCAACCGGTTTAGCCTTGAAGAAAAC
CGCACAAGAATTTGAAAAAGCGTTTGCACAAGCAACCGTTTATCAAGGCGGTGCAACGGCTGCACAACAAGCTGAAGGCT
CAGCAAGCGGTGAGAAAAAAGGTTTATATAAACACTTAATGACCGGTGTTTCTCATATGTTACCGTTAGTGGTTGCCGGT
GGTTTATTAATTGCGATTTCATTTATGTTCGGTATTGAAGCGTTCAAAGATGAAACCATCGCCGGCGGCTTACCAAAAGC
ATTAATGGATATCGGTGGCGGTGCGGCATTCCACTTAATGATCGCCGTATTTGCCGGTTATGTTGCTTTCTCTATCGCTG
ACCGTCCTGGGTTAGCGGTCGGTTTAATCGGCGGTATGTTGGCAACCACAGCCGGTGCGGGGATTTTAGGCGGTATCGTA
GCCGGTTTCCTTGCGGGTTATACCGTGAAATTCTTAAACGGCGTGATTCAATTACCGGCAAGTTTAACTTCGCTTAAACC
GATTTTGATTCTACCGTTATTAGGTTCTGCGATTGTCGGTTTAGCGATGATTTACCTCATCAACCCACCGGTAAAAGGCA
TCATGGACGCTTTAGTCGAATGGTTAAACTCAATGGGTCAAACCAATGCGATTATCTTAGGTGCAATCCTCGGTGCAATG
ATGTGTGTAGATATGGGTGGTCCGGTAAATAAAGCGGCATATACCTTCGGTGTCGGTTTAATCGCTTCACAACAATATAC
GCCGATGGCGGCGGTTATGGCGGCTGGCATGGTTCCGCCTATCGGTATGGCGATTGCAACCTTAATCGCACGTAATAAAT
TTACCACTAACCAACGTGATGCAGGTAAAGCCTCGTTCGTATTAGGCTTATGCTTTATTTCGGAAGGTGCGTTACCGTTT
GTAGCGGCGGATCCGGTTCGTGTAATTGCAACCTCTATTATTGGTGGTGCGGCAGCCGGTGCAATTTCAATGGCATTCGG
TATTACGCTTCAAGCACCGCACGGCGGTTTATTTGTGATTCCGTTCGTTTCACAACCGTTAATGTATTTAGCGGCAATCG
CAATCGGCTCAGTGGTAACCGGTGTAATTTATGCAACTATCAAACAAAAAGCATCAGAATAA

Upstream 100 bases:

>100_bases
ATTAACCCGATTAAGCTAATAATTTGTCATCAAATCTCCCCCTACCCCTCTTTACTAAAGAGGGGGGAATAAATTTTCTC
AAACTTATAAGGATCTCAAA

Downstream 100 bases:

>100_bases
TTTTCTTGATGATAAAAAACGGAGCTTGTTGGCTCCGTTTTTTTCTCCAATATATCTCGTTTTTTCTGAAAACTTTTGTA
AGTTTGCTAGGAAATTAGAC

Product: PTS system fructose-specific EIIBC component

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNISFVFPQSLGKARAFLVNEVLSAAAKQQGHQVVNAEQADFVVLFNDEIPTIAAGKQGAVVNLDQAFSQPEATLKQAVE
NAKTFANATVSSASNSGMKNIVAVTACPTGVAHTFMSAEAIENYAKAQGWNVKVETRGQVGAGNPISAEEIAAADLVFVA
ADIDVDLDKFKGKPMYRTSTGLALKKTAQEFEKAFAQATVYQGGATAAQQAEGSASGEKKGLYKHLMTGVSHMLPLVVAG
GLLIAISFMFGIEAFKDETIAGGLPKALMDIGGGAAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIV
AGFLAGYTVKFLNGVIQLPASLTSLKPILILPLLGSAIVGLAMIYLINPPVKGIMDALVEWLNSMGQTNAIILGAILGAM
MCVDMGGPVNKAAYTFGVGLIASQQYTPMAAVMAAGMVPPIGMAIATLIARNKFTTNQRDAGKASFVLGLCFISEGALPF
VAADPVRVIATSIIGGAAAGAISMAFGITLQAPHGGLFVIPFVSQPLMYLAAIAIGSVVTGVIYATIKQKASE

Sequences:

>Translated_553_residues
MNISFVFPQSLGKARAFLVNEVLSAAAKQQGHQVVNAEQADFVVLFNDEIPTIAAGKQGAVVNLDQAFSQPEATLKQAVE
NAKTFANATVSSASNSGMKNIVAVTACPTGVAHTFMSAEAIENYAKAQGWNVKVETRGQVGAGNPISAEEIAAADLVFVA
ADIDVDLDKFKGKPMYRTSTGLALKKTAQEFEKAFAQATVYQGGATAAQQAEGSASGEKKGLYKHLMTGVSHMLPLVVAG
GLLIAISFMFGIEAFKDETIAGGLPKALMDIGGGAAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIV
AGFLAGYTVKFLNGVIQLPASLTSLKPILILPLLGSAIVGLAMIYLINPPVKGIMDALVEWLNSMGQTNAIILGAILGAM
MCVDMGGPVNKAAYTFGVGLIASQQYTPMAAVMAAGMVPPIGMAIATLIARNKFTTNQRDAGKASFVLGLCFISEGALPF
VAADPVRVIATSIIGGAAAGAISMAFGITLQAPHGGLFVIPFVSQPLMYLAAIAIGSVVTGVIYATIKQKASE
>Mature_553_residues
MNISFVFPQSLGKARAFLVNEVLSAAAKQQGHQVVNAEQADFVVLFNDEIPTIAAGKQGAVVNLDQAFSQPEATLKQAVE
NAKTFANATVSSASNSGMKNIVAVTACPTGVAHTFMSAEAIENYAKAQGWNVKVETRGQVGAGNPISAEEIAAADLVFVA
ADIDVDLDKFKGKPMYRTSTGLALKKTAQEFEKAFAQATVYQGGATAAQQAEGSASGEKKGLYKHLMTGVSHMLPLVVAG
GLLIAISFMFGIEAFKDETIAGGLPKALMDIGGGAAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIV
AGFLAGYTVKFLNGVIQLPASLTSLKPILILPLLGSAIVGLAMIYLINPPVKGIMDALVEWLNSMGQTNAIILGAILGAM
MCVDMGGPVNKAAYTFGVGLIASQQYTPMAAVMAAGMVPPIGMAIATLIARNKFTTNQRDAGKASFVLGLCFISEGALPF
VAADPVRVIATSIIGGAAAGAISMAFGITLQAPHGGLFVIPFVSQPLMYLAAIAIGSVVTGVIYATIKQKASE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=557, Percent_Identity=57.9892280071813, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1786951, Length=437, Percent_Identity=34.0961098398169, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082348, Length=433, Percent_Identity=33.256351039261, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1790386, Length=313, Percent_Identity=41.5335463258786, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1788729, Length=392, Percent_Identity=27.8061224489796, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790387, Length=90, Percent_Identity=50, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788730, Length=102, Percent_Identity=35.2941176470588, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790390, Length=68, Percent_Identity=42.6470588235294, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 56769; Mature: 56769

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISFVFPQSLGKARAFLVNEVLSAAAKQQGHQVVNAEQADFVVLFNDEIPTIAAGKQGA
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCEEECCCCCC
VVNLDQAFSQPEATLKQAVENAKTFANATVSSASNSGMKNIVAVTACPTGVAHTFMSAEA
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHH
IENYAKAQGWNVKVETRGQVGAGNPISAEEIAAADLVFVAADIDVDLDKFKGKPMYRTST
HHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHEEEEEECCCCCHHHHCCCCCEECCC
GLALKKTAQEFEKAFAQATVYQGGATAAQQAEGSASGEKKGLYKHLMTGVSHMLPLVVAG
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLLIAISFMFGIEAFKDETIAGGLPKALMDIGGGAAFHLMIAVFAGYVAFSIADRPGLAV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCHH
GLIGGMLATTAGAGILGGIVAGFLAGYTVKFLNGVIQLPASLTSLKPILILPLLGSAIVG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHH
LAMIYLINPPVKGIMDALVEWLNSMGQTNAIILGAILGAMMCVDMGGPVNKAAYTFGVGL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IASQQYTPMAAVMAAGMVPPIGMAIATLIARNKFTTNQRDAGKASFVLGLCFISEGALPF
EECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCE
VAADPVRVIATSIIGGAAAGAISMAFGITLQAPHGGLFVIPFVSQPLMYLAAIAIGSVVT
EECCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEEHHHCCHHHHHHHHHHHHHHH
GVIYATIKQKASE
HHHHHHHHHHCCC
>Mature Secondary Structure
MNISFVFPQSLGKARAFLVNEVLSAAAKQQGHQVVNAEQADFVVLFNDEIPTIAAGKQGA
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCEEECCCCCC
VVNLDQAFSQPEATLKQAVENAKTFANATVSSASNSGMKNIVAVTACPTGVAHTFMSAEA
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHH
IENYAKAQGWNVKVETRGQVGAGNPISAEEIAAADLVFVAADIDVDLDKFKGKPMYRTST
HHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHEEEEEECCCCCHHHHCCCCCEECCC
GLALKKTAQEFEKAFAQATVYQGGATAAQQAEGSASGEKKGLYKHLMTGVSHMLPLVVAG
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLLIAISFMFGIEAFKDETIAGGLPKALMDIGGGAAFHLMIAVFAGYVAFSIADRPGLAV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCHH
GLIGGMLATTAGAGILGGIVAGFLAGYTVKFLNGVIQLPASLTSLKPILILPLLGSAIVG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHH
LAMIYLINPPVKGIMDALVEWLNSMGQTNAIILGAILGAMMCVDMGGPVNKAAYTFGVGL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IASQQYTPMAAVMAAGMVPPIGMAIATLIARNKFTTNQRDAGKASFVLGLCFISEGALPF
EECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCE
VAADPVRVIATSIIGGAAAGAISMAFGITLQAPHGGLFVIPFVSQPLMYLAAIAIGSVVT
EECCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEEHHHCCHHHHHHHHHHHHHHH
GVIYATIKQKASE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]