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The map label for this gene is cysK [H]

Identifier: 126207736

GI number: 126207736

Start: 269205

End: 270155

Strand: Reverse

Name: cysK [H]

Synonym: APL_0248

Alternate gene names: 126207736

Gene position: 270155-269205 (Counterclockwise)

Preceding gene: 126207737

Following gene: 126207735

Centisome position: 11.88

GC content: 44.58

Gene sequence:

>951_bases
ATGACAATCTATGCAGACAACTCATATACTATCGGTAATACCCCGATTGTTCGTTTAAAAAACTTCGGTCAAAACGGCAA
CTTATTAGTAAAAATCGAATCTCGTAACCCAAGTTTTAGCGTGAAATGCCGCATTGGTGCTAATATGGTATGGCAAGCGG
AAAAAGACGGTATTCTGACCAAAGGCAAAGAAATCGTAGATGCGACCAGCGGTAATACCGGTATCGCTCTTGCTTACGTG
GCAGCGGCTCGTGGGTATAAAATTACCCTAACTATGCCGGAAACGATGAGTACCGAACGTAAACGTTTATTACGCGGTTT
AGGTGTAAACTTGGTTTTAACCGAAGGCTCGAAAGGGATGAAAGGGGCGATCTCTAAAGCGGAAGAAATCGTCGCAAGCG
ATCCGAACCGTTATGTGATTTTAAAACAGTTTGAAAATCCGGCGAACCCGGCAATTCACCAACAAACAACCGGTCCGGAA
ATTTGGAATGCGACCGAAGGTAAAGTGGATGTGATCGTAGCAGGCGTGGGAACTGGCGGTACGATTACCGGTATTTCTCG
CTATATTAAACAAGATCAAGGCAAACAGATTATTTCCGTTGCGGTTGAACCGAAAGAATCACCGGTTATTACACAAACCT
TAAACGGCGAAGAAGTTAAACCGGGTCCGCATAAAATCCAAGGTATTGGCGCAGGTTTTATTCCGAAAAACTTAGACTTA
AGTTTAATCGACCGTGTAGAACAAGTTTCCAGCGAAGAAGCGATTGCAACCGCACGCCGAATTATGGCGGAAGAAGGTAT
CTTAGTCGGTATCTCGTCCGGTGCAGCGGTAGCAGCGGCGGATCGTTTAGCGAAGTTACCGGAATTTGCCGATAAACAGA
TCGTAGCGATTTTACCGTCCGCTTCCGAACGTTATTTAAGCACCTTATTATTTGAAGGTGTAGAAGTTTAA

Upstream 100 bases:

>100_bases
TCCTTTCCGTTATATTAAATGATTTTTCGCCACTAACATCTTTCGGAAAAATATCGTATGCTTGCTAAGAAATCTTAATT
CAAACTAAAGGAACGACCCT

Downstream 100 bases:

>100_bases
TTTTCTCTTCTTTCTTTAAAATGCCGGTATAACGCCGGCATTTTTCGTTTAGAAATGCTCGTAAGCGGTCTATTTTTGCA
AAGAATTTGCAATTTGGATA

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTIYADNSYTIGNTPIVRLKNFGQNGNLLVKIESRNPSFSVKCRIGANMVWQAEKDGILTKGKEIVDATSGNTGIALAYV
AAARGYKITLTMPETMSTERKRLLRGLGVNLVLTEGSKGMKGAISKAEEIVASDPNRYVILKQFENPANPAIHQQTTGPE
IWNATEGKVDVIVAGVGTGGTITGISRYIKQDQGKQIISVAVEPKESPVITQTLNGEEVKPGPHKIQGIGAGFIPKNLDL
SLIDRVEQVSSEEAIATARRIMAEEGILVGISSGAAVAAADRLAKLPEFADKQIVAILPSASERYLSTLLFEGVEV

Sequences:

>Translated_316_residues
MTIYADNSYTIGNTPIVRLKNFGQNGNLLVKIESRNPSFSVKCRIGANMVWQAEKDGILTKGKEIVDATSGNTGIALAYV
AAARGYKITLTMPETMSTERKRLLRGLGVNLVLTEGSKGMKGAISKAEEIVASDPNRYVILKQFENPANPAIHQQTTGPE
IWNATEGKVDVIVAGVGTGGTITGISRYIKQDQGKQIISVAVEPKESPVITQTLNGEEVKPGPHKIQGIGAGFIPKNLDL
SLIDRVEQVSSEEAIATARRIMAEEGILVGISSGAAVAAADRLAKLPEFADKQIVAILPSASERYLSTLLFEGVEV
>Mature_315_residues
TIYADNSYTIGNTPIVRLKNFGQNGNLLVKIESRNPSFSVKCRIGANMVWQAEKDGILTKGKEIVDATSGNTGIALAYVA
AARGYKITLTMPETMSTERKRLLRGLGVNLVLTEGSKGMKGAISKAEEIVASDPNRYVILKQFENPANPAIHQQTTGPEI
WNATEGKVDVIVAGVGTGGTITGISRYIKQDQGKQIISVAVEPKESPVITQTLNGEEVKPGPHKIQGIGAGFIPKNLDLS
LIDRVEQVSSEEAIATARRIMAEEGILVGISSGAAVAAADRLAKLPEFADKQIVAILPSASERYLSTLLFEGVEV

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=323, Percent_Identity=39.938080495356, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI295821200, Length=323, Percent_Identity=39.938080495356, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4557415, Length=323, Percent_Identity=39.938080495356, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1788754, Length=310, Percent_Identity=71.2903225806452, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=41.1184210526316, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=50, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17562970, Length=312, Percent_Identity=48.7179487179487, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=304, Percent_Identity=49.3421052631579, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=52.9605263157895, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17561720, Length=309, Percent_Identity=48.8673139158576, Blast_Score=244, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI32566672, Length=223, Percent_Identity=47.9820627802691, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=321, Percent_Identity=40.1869158878505, Blast_Score=151, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=314, Percent_Identity=35.9872611464968, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17561716, Length=125, Percent_Identity=48, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=35.5555555555556, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321449, Length=331, Percent_Identity=32.9305135951662, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24643623, Length=308, Percent_Identity=37.012987012987, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI20129101, Length=308, Percent_Identity=37.012987012987, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33743; Mature: 33612

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYADNSYTIGNTPIVRLKNFGQNGNLLVKIESRNPSFSVKCRIGANMVWQAEKDGILT
CEEECCCCEEECCCCEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCEEEEECCCCCEE
KGKEIVDATSGNTGIALAYVAAARGYKITLTMPETMSTERKRLLRGLGVNLVLTEGSKGM
CCCEEEEECCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCC
KGAISKAEEIVASDPNRYVILKQFENPANPAIHQQTTGPEIWNATEGKVDVIVAGVGTGG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
TITGISRYIKQDQGKQIISVAVEPKESPVITQTLNGEEVKPGPHKIQGIGAGFIPKNLDL
HHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCH
SLIDRVEQVSSEEAIATARRIMAEEGILVGISSGAAVAAADRLAKLPEFADKQIVAILPS
HHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECC
ASERYLSTLLFEGVEV
CHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIYADNSYTIGNTPIVRLKNFGQNGNLLVKIESRNPSFSVKCRIGANMVWQAEKDGILT
EEECCCCEEECCCCEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCEEEEECCCCCEE
KGKEIVDATSGNTGIALAYVAAARGYKITLTMPETMSTERKRLLRGLGVNLVLTEGSKGM
CCCEEEEECCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCC
KGAISKAEEIVASDPNRYVILKQFENPANPAIHQQTTGPEIWNATEGKVDVIVAGVGTGG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
TITGISRYIKQDQGKQIISVAVEPKESPVITQTLNGEEVKPGPHKIQGIGAGFIPKNLDL
HHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCH
SLIDRVEQVSSEEAIATARRIMAEEGILVGISSGAAVAAADRLAKLPEFADKQIVAILPS
HHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECC
ASERYLSTLLFEGVEV
CHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]