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The map label for this gene is glyA

Identifier: 126207699

GI number: 126207699

Start: 228822

End: 230087

Strand: Reverse

Name: glyA

Synonym: APL_0211

Alternate gene names: 126207699

Gene position: 230087-228822 (Counterclockwise)

Preceding gene: 126207700

Following gene: 126207698

Centisome position: 10.12

GC content: 44.55

Gene sequence:

>1266_bases
ATGTTTACAGCAAATATGAACATTCAAGATTACGACCCTATTTTATGGCAGGCGATTGAAAACGAAAATCGCCGTCAAGA
AGAACATATTGAGCTGATTGCTTCGGAAAACTATGCAAGCCCTCGAGTGATGCAGGCGCAAGGTTCGCAATTTACTAATA
AATATGCCGAAGGTTATCCGGGAAAACGCTATTACGGCGGTTGCGAATATGCGGATATTGTCGAGCAATTAGCGATTGAT
CGTGCGAAACAGTTATTCGGTGCAGACTATGTAAACGTCCAACCGCATTCAGGCTCACAAGCGAATGCAGCTGTTTACGG
CGCATTAATTCAACCGAACGATACCATTTTAGGTATGGACTTAGCACATGGAGGCCATTTAACGCACGGAGCAAAAGTGA
GCTTCTCAGGCAAAATTTATAATTCTGTCCTCTACGGCATCACAGCCGAAGGCTTAATTGATTATGAAGACGTCCGTCAA
AAAGCGCTTGAGTGTAAGCCAAAAATGATTGTTGCCGGCTTTTCCGCTTATTCACAAATCGTAGATTGGGCGAAAATGCG
TGAAATTGCCGATGAAGTCGGTGCTTATTTATTCGTCGATATGGCGCACGTTGCCGGTTTAATTGCGGCAGGCGTTTACC
CAAGCCCGCTTCCTTACGCTCACGTTGTTACAACCACAACCCATAAAACTTTAGGCGGTCCGCGCGGCGGTTTAATTCTT
TCCGCTTGTGGCGATGAAGAAATTTATAAAAAACTGCAATCTTCGGTTTTCCCGGCAAACCAAGGAGGGCCATTAGTGCA
TATTATTGCGGCGAAAGCGGTTTGCTTTAAAGAAGCGCTTGAGCCTGAATATAAAATCTATCAACAAAATGTAGTTAAAA
ATGCGAAAGCAATGGTAGAAGTATTCAAACAACGCAGCTATGAAGTAATTTCAAACGGTACCGAAAACCACTTATTCTTA
GTCAGCTTTGTAAAACAAGGCTTAACCGGTAAAGCGGCAGATGCGGCGTTAGGTCAAGCGAATATCACCGTAAATAAAAA
TTCCGTGCCGAATGATCCGCAAAAACCGTTTATTACTTCCGGTATTCGCATCGGTACGCCTGCCGTTACTCGTCGCGGTT
TCAAAGAAGCGGACGTTCAAGCCCTTGCCGGCTGGATGTGCGACGTATTGGACAGCATCGGTAAAGACAATCACGAACAA
GTAATTGCGGAAACCAAAGCGAAAGTGCTTGATATTTGCGCTCGCTTGCCGGTATATGCCAAATAA

Upstream 100 bases:

>100_bases
AAGAGCTACCATCATGTTTAATAAATGTGTAGAATGCAGTTAAACTCATTTGCGCAAACGTTTGCGCAACTTTATTTATC
ATCAAACAGAGGACTTCCAT

Downstream 100 bases:

>100_bases
GATTTCTAACAAACACCAAGCCCCGAGCTAATGATCTGCACCCCAAAAGTTGGACTAAACCTCCAACGGATTAAGGTGCA
GATTTTTTATGACTAAATAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MFTANMNIQDYDPILWQAIENENRRQEEHIELIASENYASPRVMQAQGSQFTNKYAEGYPGKRYYGGCEYADIVEQLAID
RAKQLFGADYVNVQPHSGSQANAAVYGALIQPNDTILGMDLAHGGHLTHGAKVSFSGKIYNSVLYGITAEGLIDYEDVRQ
KALECKPKMIVAGFSAYSQIVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPYAHVVTTTTHKTLGGPRGGLIL
SACGDEEIYKKLQSSVFPANQGGPLVHIIAAKAVCFKEALEPEYKIYQQNVVKNAKAMVEVFKQRSYEVISNGTENHLFL
VSFVKQGLTGKAADAALGQANITVNKNSVPNDPQKPFITSGIRIGTPAVTRRGFKEADVQALAGWMCDVLDSIGKDNHEQ
VIAETKAKVLDICARLPVYAK

Sequences:

>Translated_421_residues
MFTANMNIQDYDPILWQAIENENRRQEEHIELIASENYASPRVMQAQGSQFTNKYAEGYPGKRYYGGCEYADIVEQLAID
RAKQLFGADYVNVQPHSGSQANAAVYGALIQPNDTILGMDLAHGGHLTHGAKVSFSGKIYNSVLYGITAEGLIDYEDVRQ
KALECKPKMIVAGFSAYSQIVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPYAHVVTTTTHKTLGGPRGGLIL
SACGDEEIYKKLQSSVFPANQGGPLVHIIAAKAVCFKEALEPEYKIYQQNVVKNAKAMVEVFKQRSYEVISNGTENHLFL
VSFVKQGLTGKAADAALGQANITVNKNSVPNDPQKPFITSGIRIGTPAVTRRGFKEADVQALAGWMCDVLDSIGKDNHEQ
VIAETKAKVLDICARLPVYAK
>Mature_421_residues
MFTANMNIQDYDPILWQAIENENRRQEEHIELIASENYASPRVMQAQGSQFTNKYAEGYPGKRYYGGCEYADIVEQLAID
RAKQLFGADYVNVQPHSGSQANAAVYGALIQPNDTILGMDLAHGGHLTHGAKVSFSGKIYNSVLYGITAEGLIDYEDVRQ
KALECKPKMIVAGFSAYSQIVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPYAHVVTTTTHKTLGGPRGGLIL
SACGDEEIYKKLQSSVFPANQGGPLVHIIAAKAVCFKEALEPEYKIYQQNVVKNAKAMVEVFKQRSYEVISNGTENHLFL
VSFVKQGLTGKAADAALGQANITVNKNSVPNDPQKPFITSGIRIGTPAVTRRGFKEADVQALAGWMCDVLDSIGKDNHEQ
VIAETKAKVLDICARLPVYAK

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=407, Percent_Identity=44.4717444717445, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI261862350, Length=407, Percent_Identity=44.4717444717445, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI261862348, Length=407, Percent_Identity=44.4717444717445, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI19923315, Length=407, Percent_Identity=44.4717444717445, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI261862346, Length=406, Percent_Identity=43.3497536945813, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=44.3890274314214, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=42.9305912596401, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1788902, Length=420, Percent_Identity=75.7142857142857, Blast_Score=648, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=411, Percent_Identity=44.7688564476886, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25144729, Length=411, Percent_Identity=44.7688564476886, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=44.5273631840796, Blast_Score=332, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=438, Percent_Identity=41.5525114155251, Blast_Score=324, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640005, Length=396, Percent_Identity=46.969696969697, Blast_Score=357, Evalue=7e-99,
Organism=Drosophila melanogaster, GI221329721, Length=396, Percent_Identity=46.969696969697, Blast_Score=357, Evalue=9e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ACTP2 (A3MYT3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052924.1
- ProteinModelPortal:   A3MYT3
- SMR:   A3MYT3
- STRING:   A3MYT3
- GeneID:   4849582
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0211
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   GGLIMTK
- PhylomeDB:   A3MYT3
- ProtClustDB:   PRK00011
- BioCyc:   APLE416269:APL_0211-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45984; Mature: 45984

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTANMNIQDYDPILWQAIENENRRQEEHIELIASENYASPRVMQAQGSQFTNKYAEGYP
CEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEHHCCCHHHHHHHCCCC
GKRYYGGCEYADIVEQLAIDRAKQLFGADYVNVQPHSGSQANAAVYGALIQPNDTILGMD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGAKVSFSGKIYNSVLYGITAEGLIDYEDVRQKALECKPKMIVAGFSAYSQI
ECCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPYAHVVTTTTHKTLGGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCCCCEEE
SACGDEEIYKKLQSSVFPANQGGPLVHIIAAKAVCFKEALEPEYKIYQQNVVKNAKAMVE
EECCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFKQRSYEVISNGTENHLFLVSFVKQGLTGKAADAALGQANITVNKNSVPNDPQKPFITS
HHHHCCHHHHCCCCCCCEEHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCC
GIRIGTPAVTRRGFKEADVQALAGWMCDVLDSIGKDNHEQVIAETKAKVLDICARLPVYA
CEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
K
C
>Mature Secondary Structure
MFTANMNIQDYDPILWQAIENENRRQEEHIELIASENYASPRVMQAQGSQFTNKYAEGYP
CEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEHHCCCHHHHHHHCCCC
GKRYYGGCEYADIVEQLAIDRAKQLFGADYVNVQPHSGSQANAAVYGALIQPNDTILGMD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGAKVSFSGKIYNSVLYGITAEGLIDYEDVRQKALECKPKMIVAGFSAYSQI
ECCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPYAHVVTTTTHKTLGGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCCCCEEE
SACGDEEIYKKLQSSVFPANQGGPLVHIIAAKAVCFKEALEPEYKIYQQNVVKNAKAMVE
EECCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFKQRSYEVISNGTENHLFLVSFVKQGLTGKAADAALGQANITVNKNSVPNDPQKPFITS
HHHHCCHHHHCCCCCCCEEHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCC
GIRIGTPAVTRRGFKEADVQALAGWMCDVLDSIGKDNHEQVIAETKAKVLDICARLPVYA
CEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA