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The map label for this gene is ppnK [H]

Identifier: 126207700

GI number: 126207700

Start: 230318

End: 231205

Strand: Reverse

Name: ppnK [H]

Synonym: APL_0212

Alternate gene names: 126207700

Gene position: 231205-230318 (Counterclockwise)

Preceding gene: 126207701

Following gene: 126207699

Centisome position: 10.17

GC content: 42.91

Gene sequence:

>888_bases
ATGAAACCTGAAGAACGATTTTTTGAATGTATTGCGATTGTCGGTAAGCCTCGGCATGAGATTGCGTTGGAAACACATTT
AGCGGTTTATAATTGGTTAAAAGACCGCCATTATCGTGTGTTAGTCGAAAAAAGTATCGCACAGCAATTAAATCTGCCCG
AAGCAAGAAGCCTAGAGGAAATCGGGCAACAAGCGAATTTGGTTATTGTGATCGGTGGAGACGGCAATATGCTCGGTATG
GCTCGCAGGTTAGCCGAATATCAAGTACCGCTTATCGGTATTAACCGAGGTAACCTAGGTTTTTTAACCGATATTGCCCC
ACATTCAACCTTCGAACAATTACACAATTGCATCGAACGAGGCGAGTTTGTGATTGAAGAACGTTTTTTACTTGAAGCGC
GTATTGAGCGTAACGGTAAAATTATTGCAACCAATAATGCACTGAATGAAGTGGTCATTCATCCGACTCAAGTGGCTCGT
ATTATTGAATTCGAGGTTTATATCGACGGTAAATTCGCATTTTCCCAGCGTTCTGACGGTTTAATAATCAGCACGCCGAC
CGGCTCTACCGCTTATTCGCTTTCTGCCGGCGGTCCGATTTTAACCCCAAATATGAATGCAATTGCGCTTGTGCCGATGC
ATCCGCATACGCTTTCATCCCGTCCTTTGGTTATTGACGGCGACAGCCAGATTTCTTTGCGTTTCGCACAATATAATCAG
CCTAGCTTGGAAGTGAGCTGCGACGGTCAATACGATTTACCCTTTACACCGGAAGACCGCATTATTGTAGAGAAAAGCCC
GAATAAATTACGTCTTCTGCATTTAAAAGATTATAACTATTTTACCGTGCTGGGTTCTAAACTCGGTTGGTCAAGTAAAT
TATTTTAA

Upstream 100 bases:

>100_bases
GCAGCCATAACAAGCGGTCATTTTTTATGAATTTTTTACATATCTTATCAATGAAAAAAAGGATAGAATTCAATAATTCT
TATTTTGAGAGTAGAAAAAG

Downstream 100 bases:

>100_bases
TTTCTTTTCGCTTAGGCACTCCGTCCGAGTGCCTTTTGTCGGTAAATATTCCACAAAATTCAACCGCTTACCCTTCAATT
AATTTTTTTCATTATTCCTA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKPEERFFECIAIVGKPRHEIALETHLAVYNWLKDRHYRVLVEKSIAQQLNLPEARSLEEIGQQANLVIVIGGDGNMLGM
ARRLAEYQVPLIGINRGNLGFLTDIAPHSTFEQLHNCIERGEFVIEERFLLEARIERNGKIIATNNALNEVVIHPTQVAR
IIEFEVYIDGKFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPNMNAIALVPMHPHTLSSRPLVIDGDSQISLRFAQYNQ
PSLEVSCDGQYDLPFTPEDRIIVEKSPNKLRLLHLKDYNYFTVLGSKLGWSSKLF

Sequences:

>Translated_295_residues
MKPEERFFECIAIVGKPRHEIALETHLAVYNWLKDRHYRVLVEKSIAQQLNLPEARSLEEIGQQANLVIVIGGDGNMLGM
ARRLAEYQVPLIGINRGNLGFLTDIAPHSTFEQLHNCIERGEFVIEERFLLEARIERNGKIIATNNALNEVVIHPTQVAR
IIEFEVYIDGKFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPNMNAIALVPMHPHTLSSRPLVIDGDSQISLRFAQYNQ
PSLEVSCDGQYDLPFTPEDRIIVEKSPNKLRLLHLKDYNYFTVLGSKLGWSSKLF
>Mature_295_residues
MKPEERFFECIAIVGKPRHEIALETHLAVYNWLKDRHYRVLVEKSIAQQLNLPEARSLEEIGQQANLVIVIGGDGNMLGM
ARRLAEYQVPLIGINRGNLGFLTDIAPHSTFEQLHNCIERGEFVIEERFLLEARIERNGKIIATNNALNEVVIHPTQVAR
IIEFEVYIDGKFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPNMNAIALVPMHPHTLSSRPLVIDGDSQISLRFAQYNQ
PSLEVSCDGQYDLPFTPEDRIIVEKSPNKLRLLHLKDYNYFTVLGSKLGWSSKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=304, Percent_Identity=27.3026315789474, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1788968, Length=290, Percent_Identity=56.8965517241379, Blast_Score=330, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=29.6296296296296, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6322509, Length=244, Percent_Identity=30.327868852459, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6325068, Length=217, Percent_Identity=31.7972350230415, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28573828, Length=317, Percent_Identity=25.2365930599369, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573826, Length=317, Percent_Identity=25.2365930599369, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573832, Length=317, Percent_Identity=25.2365930599369, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573830, Length=317, Percent_Identity=25.2365930599369, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161077047, Length=317, Percent_Identity=25.2365930599369, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24653422, Length=280, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281363321, Length=280, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281363323, Length=280, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653424, Length=280, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20129957, Length=280, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 33181; Mature: 33181

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEERFFECIAIVGKPRHEIALETHLAVYNWLKDRHYRVLVEKSIAQQLNLPEARSLEE
CCCHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHCCCEEEEEEHHHHHHCCCCCCCCHHH
IGQQANLVIVIGGDGNMLGMARRLAEYQVPLIGINRGNLGFLTDIAPHSTFEQLHNCIER
HCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHC
GEFVIEERFLLEARIERNGKIIATNNALNEVVIHPTQVARIIEFEVYIDGKFAFSQRSDG
CCEEEEHHHEEEEEECCCCEEEEECCCCCEEEECHHHEEEEEEEEEEECCEEEECCCCCC
LIISTPTGSTAYSLSAGGPILTPNMNAIALVPMHPHTLSSRPLVIDGDSQISLRFAQYNQ
EEEECCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEEEECCC
PSLEVSCDGQYDLPFTPEDRIIVEKSPNKLRLLHLKDYNYFTVLGSKLGWSSKLF
CCEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKPEERFFECIAIVGKPRHEIALETHLAVYNWLKDRHYRVLVEKSIAQQLNLPEARSLEE
CCCHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHCCCEEEEEEHHHHHHCCCCCCCCHHH
IGQQANLVIVIGGDGNMLGMARRLAEYQVPLIGINRGNLGFLTDIAPHSTFEQLHNCIER
HCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHC
GEFVIEERFLLEARIERNGKIIATNNALNEVVIHPTQVARIIEFEVYIDGKFAFSQRSDG
CCEEEEHHHEEEEEECCCCEEEEECCCCCEEEECHHHEEEEEEEEEEECCEEEECCCCCC
LIISTPTGSTAYSLSAGGPILTPNMNAIALVPMHPHTLSSRPLVIDGDSQISLRFAQYNQ
EEEECCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEEEECCC
PSLEVSCDGQYDLPFTPEDRIIVEKSPNKLRLLHLKDYNYFTVLGSKLGWSSKLF
CCEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA