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The map label for this gene is mrcA [H]

Identifier: 126207683

GI number: 126207683

Start: 214614

End: 217181

Strand: Reverse

Name: mrcA [H]

Synonym: APL_0195

Alternate gene names: 126207683

Gene position: 217181-214614 (Counterclockwise)

Preceding gene: 126207693

Following gene: 126207682

Centisome position: 9.55

GC content: 44.24

Gene sequence:

>2568_bases
ATGAAGATCGCAAAAATAATATTAAGTGCCTTACTTTCACTTATTATTTTAGGTGGCATCGCCGCCGGCTTAGCTTATAT
ACATATCAGAGACAGTTTGCCGAATGTGTCTAGCTTAAAAACGGTTGAGTTGCAGCAGCCGATGCAGATCTTTACTGCAG
ACGGCAAATTAATCGGTGAAGTCGGCGAACAACGTCGTATCCCAGTGAAATTAGCCGAAATTCCGCAAACACTGATTGAT
GCGGTAATTGCGACCGAAGACGTGCGCTTTTATGACCATAAAGGTATTGATCCGAAAGGGATCGTTCGAGCGATTTGGCG
TTCCAGTCAAGGCGATACCCAAGGTGCAAGTACCATTACCCAACAGTTGGCTCGTAACTTTTTCTTAACGCCGGAACGTA
ATTTGGAACGTAAGCTCAAAGAAGCGATTCTTGCGCTTGAAATCGAGAAAGTACTAACGAAAGACGAAATTCTTGAGCTT
TACCTCAATAAAATTTATCTAGGTTATCGTTCACACGGCGTTGCCGCCGCAGCCAAAACCTATTTTGGTAAGAACCTCGA
TCAACTGACTTTAGGTGAAATGGCGATTATTGCCGGCTTACCGAAAGCACCATCGACCATGAACCCGCTTTATTCGGTAA
AACGTGCTGAAGCGCGCCGTAATGTGGTATTGGGTCGAATGTTAGAAGTCGGTAAAATTACTCAACAGCAATATGATCAA
GCGAAAGCGGAAACGGTAAAAGCGACGTTTTACGGTACCGCTTTGGAATTCCGAGCCGATTACGTTACCGAGATGGTTCG
CCAAGAAATGGTTAAACGTTACGGTGAGGACGTGGCATATAATAAAGGTTTTAAAGTGTATGCGACCGTGCTTTCAACCG
ACCAAAAAGCGGCGCAAGACGCATTAAGCGAGAACTTGATTAATTATGATCGCCGTCACGGTTGGCGCGGGGCCGAAAAA
CTTTGGAGCGGTAAAGAGGCTTGGGACGACGAGAAAATTATCGACCATCTAAGTAAATTGCCGAATTCCGATCCGTTTAT
CGGTGCGGCTGTTATGGCGGTCGGAAAAAACGGCTATACCATTATGTTACCTAACGGCGATAAAGAAGAATTACAAAAAT
CGAACGCCGTATTCGGCAAACAAATCAAGCTCAACGTAGGCGAACAAATTTGGGTTCGTAAATATAAAAACGGTAAAGAT
TGGCAACTCGGTCAGATTCCTGAAGTGAATTCGGCATTAGTTTCGTTAAACAGCGATAACGGTGCGATTGAAGCGATTGT
CGGCGGTTTTAGTTTTGAACAAAGTCGTTTTAATCGAGCGACACAATCGCTCGTGCAAGTCGGTTCATCGATTAAACCGT
TTATTTATGCGGCGGCTATGAATAAAGGTTTAAGTCTTTCCACGACACTAAGCGACTCGCCGATTACCATTACTAAAAAA
GGACAAAAACCGTGGACGCCTAAAAATGCGGATAATCGTTATGAAGGCCCGTTACGCTTACGTGTCGCACTCGGTAAGTC
TAAAAATATGATTGCCATTCGCGCATTACAGATGGCGGGTGTAGATTACGTTGCGGAATATCTTGAACGTTTCGGTTTCA
ATCGAGATCAATTTGTAGCGACCGAAGCGCTCGCTTTAGGCGCAGCTTCATTTACTCCGCTGGAAATGGCAAGAGGTTAT
GCCGTGTTTGATAACGGCGGTTACTTAATCGAACCTTATATTATTGAACGTATTTTAGATAATCAGGGTAACGAATTATT
TAAAGCGAATCCGGCGATTGCTTGTATGGATTGTAATATCGGCGTTAAATATCCTGAACCGACCTATTTCGATGGCGTGA
AGATTCATGATGAAACGCAATTACAAGGAAACTCGGAAATCTCGGAAAATGATGAAAGTAACGATACTTCGGCAGAGAAT
GACGATGAGCTCGAAATTAAACCGGCGGTAAAATCCGATGCGCCGAGTTTAATGACCGAAGCGGCACAAAACGGTACGGA
AACCCGTTATGCGCCGCGCGTTATCAGCGGCGAACTCGCCTTCCTGATGCGTAGCGCATTAAACAGTGCGATTTACGGCG
AACCGGGACAAGCGTGGAACGGAACCAGTAAAGCGCTTGCAAATGAATTTAAACGTAAAGATATCGGCGGAAAAACCGGT
ACGACCAATAATTCGAAAGTCACTTGGTATGCCGGTTTCGGTGCGAATATCGTGACCACGGTCTATGTCGGTTTCGACGA
TAATAAACGTGATTTGGGTAAAGGCGAAGCGGGAGCCAGAACCGCAATGCCGGCTTGGATTAAATATATGAAAGTGGCGT
TAGCGGATAAAAAAGAAAGAACGCCGGTAATGCCACCGAATATTATCGAAACTAAAATCGATAGCGTAAGCGGTTTCCTC
GGTAATAACTTAACCGAATACTTTATTAAAGGAACCGAACCGACGAAAAAATATATCGTAGAAAAAGCCTATAAATTACC
TCAAGAGGCGAAACCGCATTTAGAACAACGACTGGGCTTACCGCCGAAAGAATTATTAAATTCACCGGGTAGCTTCGAGT
TATTCTAA

Upstream 100 bases:

>100_bases
GCGGTTTTTTTAACATTTTTGGCTTATACTTATATTTTTAATGTAACTATATCTTGGCAATAACTTTTATTTCCGTCATT
TACCAGAAGAGAATTTTTCG

Downstream 100 bases:

>100_bases
AAAATAATTTATGAAATATTTTAGGGATTATCTCCCTATGAGGTTGAATCGGGTATGGTTTCTTGCGATAATCATACCCA
ATTATTTTGGGCAATTTGTG

Product: membrane carboxypeptidase/penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-binding protein A; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 855; Mature: 855

Protein sequence:

>855_residues
MKIAKIILSALLSLIILGGIAAGLAYIHIRDSLPNVSSLKTVELQQPMQIFTADGKLIGEVGEQRRIPVKLAEIPQTLID
AVIATEDVRFYDHKGIDPKGIVRAIWRSSQGDTQGASTITQQLARNFFLTPERNLERKLKEAILALEIEKVLTKDEILEL
YLNKIYLGYRSHGVAAAAKTYFGKNLDQLTLGEMAIIAGLPKAPSTMNPLYSVKRAEARRNVVLGRMLEVGKITQQQYDQ
AKAETVKATFYGTALEFRADYVTEMVRQEMVKRYGEDVAYNKGFKVYATVLSTDQKAAQDALSENLINYDRRHGWRGAEK
LWSGKEAWDDEKIIDHLSKLPNSDPFIGAAVMAVGKNGYTIMLPNGDKEELQKSNAVFGKQIKLNVGEQIWVRKYKNGKD
WQLGQIPEVNSALVSLNSDNGAIEAIVGGFSFEQSRFNRATQSLVQVGSSIKPFIYAAAMNKGLSLSTTLSDSPITITKK
GQKPWTPKNADNRYEGPLRLRVALGKSKNMIAIRALQMAGVDYVAEYLERFGFNRDQFVATEALALGAASFTPLEMARGY
AVFDNGGYLIEPYIIERILDNQGNELFKANPAIACMDCNIGVKYPEPTYFDGVKIHDETQLQGNSEISENDESNDTSAEN
DDELEIKPAVKSDAPSLMTEAAQNGTETRYAPRVISGELAFLMRSALNSAIYGEPGQAWNGTSKALANEFKRKDIGGKTG
TTNNSKVTWYAGFGANIVTTVYVGFDDNKRDLGKGEAGARTAMPAWIKYMKVALADKKERTPVMPPNIIETKIDSVSGFL
GNNLTEYFIKGTEPTKKYIVEKAYKLPQEAKPHLEQRLGLPPKELLNSPGSFELF

Sequences:

>Translated_855_residues
MKIAKIILSALLSLIILGGIAAGLAYIHIRDSLPNVSSLKTVELQQPMQIFTADGKLIGEVGEQRRIPVKLAEIPQTLID
AVIATEDVRFYDHKGIDPKGIVRAIWRSSQGDTQGASTITQQLARNFFLTPERNLERKLKEAILALEIEKVLTKDEILEL
YLNKIYLGYRSHGVAAAAKTYFGKNLDQLTLGEMAIIAGLPKAPSTMNPLYSVKRAEARRNVVLGRMLEVGKITQQQYDQ
AKAETVKATFYGTALEFRADYVTEMVRQEMVKRYGEDVAYNKGFKVYATVLSTDQKAAQDALSENLINYDRRHGWRGAEK
LWSGKEAWDDEKIIDHLSKLPNSDPFIGAAVMAVGKNGYTIMLPNGDKEELQKSNAVFGKQIKLNVGEQIWVRKYKNGKD
WQLGQIPEVNSALVSLNSDNGAIEAIVGGFSFEQSRFNRATQSLVQVGSSIKPFIYAAAMNKGLSLSTTLSDSPITITKK
GQKPWTPKNADNRYEGPLRLRVALGKSKNMIAIRALQMAGVDYVAEYLERFGFNRDQFVATEALALGAASFTPLEMARGY
AVFDNGGYLIEPYIIERILDNQGNELFKANPAIACMDCNIGVKYPEPTYFDGVKIHDETQLQGNSEISENDESNDTSAEN
DDELEIKPAVKSDAPSLMTEAAQNGTETRYAPRVISGELAFLMRSALNSAIYGEPGQAWNGTSKALANEFKRKDIGGKTG
TTNNSKVTWYAGFGANIVTTVYVGFDDNKRDLGKGEAGARTAMPAWIKYMKVALADKKERTPVMPPNIIETKIDSVSGFL
GNNLTEYFIKGTEPTKKYIVEKAYKLPQEAKPHLEQRLGLPPKELLNSPGSFELF
>Mature_855_residues
MKIAKIILSALLSLIILGGIAAGLAYIHIRDSLPNVSSLKTVELQQPMQIFTADGKLIGEVGEQRRIPVKLAEIPQTLID
AVIATEDVRFYDHKGIDPKGIVRAIWRSSQGDTQGASTITQQLARNFFLTPERNLERKLKEAILALEIEKVLTKDEILEL
YLNKIYLGYRSHGVAAAAKTYFGKNLDQLTLGEMAIIAGLPKAPSTMNPLYSVKRAEARRNVVLGRMLEVGKITQQQYDQ
AKAETVKATFYGTALEFRADYVTEMVRQEMVKRYGEDVAYNKGFKVYATVLSTDQKAAQDALSENLINYDRRHGWRGAEK
LWSGKEAWDDEKIIDHLSKLPNSDPFIGAAVMAVGKNGYTIMLPNGDKEELQKSNAVFGKQIKLNVGEQIWVRKYKNGKD
WQLGQIPEVNSALVSLNSDNGAIEAIVGGFSFEQSRFNRATQSLVQVGSSIKPFIYAAAMNKGLSLSTTLSDSPITITKK
GQKPWTPKNADNRYEGPLRLRVALGKSKNMIAIRALQMAGVDYVAEYLERFGFNRDQFVATEALALGAASFTPLEMARGY
AVFDNGGYLIEPYIIERILDNQGNELFKANPAIACMDCNIGVKYPEPTYFDGVKIHDETQLQGNSEISENDESNDTSAEN
DDELEIKPAVKSDAPSLMTEAAQNGTETRYAPRVISGELAFLMRSALNSAIYGEPGQAWNGTSKALANEFKRKDIGGKTG
TTNNSKVTWYAGFGANIVTTVYVGFDDNKRDLGKGEAGARTAMPAWIKYMKVALADKKERTPVMPPNIIETKIDSVSGFL
GNNLTEYFIKGTEPTKKYIVEKAYKLPQEAKPHLEQRLGLPPKELLNSPGSFELF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=857, Percent_Identity=48.8914819136523, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1786343, Length=282, Percent_Identity=35.1063829787234, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1788867, Length=184, Percent_Identity=36.4130434782609, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1789601, Length=164, Percent_Identity=37.1951219512195, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 94565; Mature: 94565

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAKIILSALLSLIILGGIAAGLAYIHIRDSLPNVSSLKTVELQQPMQIFTADGKLIGE
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEHHCCCEEEECCCHHHHH
VGEQRRIPVKLAEIPQTLIDAVIATEDVRFYDHKGIDPKGIVRAIWRSSQGDTQGASTIT
CCCCCCCCEEHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHH
QQLARNFFLTPERNLERKLKEAILALEIEKVLTKDEILELYLNKIYLGYRSHGVAAAAKT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
YFGKNLDQLTLGEMAIIAGLPKAPSTMNPLYSVKRAEARRNVVLGRMLEVGKITQQQYDQ
HHCCCCCHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAETVKATFYGTALEFRADYVTEMVRQEMVKRYGEDVAYNKGFKVYATVLSTDQKAAQD
HHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEHHCCHHHHHH
ALSENLINYDRRHGWRGAEKLWSGKEAWDDEKIIDHLSKLPNSDPFIGAAVMAVGKNGYT
HHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHEEECCCCCE
IMLPNGDKEELQKSNAVFGKQIKLNVGEQIWVRKYKNGKDWQLGQIPEVNSALVSLNSDN
EECCCCCHHHHHHHCCCCCCEEEECCCCEEEHHCCCCCCCCCCCCCCCCCCEEEEECCCC
GAIEAIVGGFSFEQSRFNRATQSLVQVGSSIKPFIYAAAMNKGLSLSTTLSDSPITITKK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEHHCCCCEEEEECCCCCEEEEEC
GQKPWTPKNADNRYEGPLRLRVALGKSKNMIAIRALQMAGVDYVAEYLERFGFNRDQFVA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHHHHCCCCHHHHH
TEALALGAASFTPLEMARGYAVFDNGGYLIEPYIIERILDNQGNELFKANPAIACMDCNI
HHHHHHCCCCCCHHHHHCCEEEEECCCEEECHHHHHHHHCCCCCCEEECCCCEEEEECCC
GVKYPEPTYFDGVKIHDETQLQGNSEISENDESNDTSAENDDELEIKPAVKSDAPSLMTE
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHH
AAQNGTETRYAPRVISGELAFLMRSALNSAIYGEPGQAWNGTSKALANEFKRKDIGGKTG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
TTNNSKVTWYAGFGANIVTTVYVGFDDNKRDLGKGEAGARTAMPAWIKYMKVALADKKER
CCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
TPVMPPNIIETKIDSVSGFLGNNLTEYFIKGTEPTKKYIVEKAYKLPQEAKPHLEQRLGL
CCCCCCCHHHHHHHHHHHHHCCCCCHHEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCC
PPKELLNSPGSFELF
CHHHHHCCCCCEECC
>Mature Secondary Structure
MKIAKIILSALLSLIILGGIAAGLAYIHIRDSLPNVSSLKTVELQQPMQIFTADGKLIGE
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEHHCCCEEEECCCHHHHH
VGEQRRIPVKLAEIPQTLIDAVIATEDVRFYDHKGIDPKGIVRAIWRSSQGDTQGASTIT
CCCCCCCCEEHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHH
QQLARNFFLTPERNLERKLKEAILALEIEKVLTKDEILELYLNKIYLGYRSHGVAAAAKT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
YFGKNLDQLTLGEMAIIAGLPKAPSTMNPLYSVKRAEARRNVVLGRMLEVGKITQQQYDQ
HHCCCCCHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAETVKATFYGTALEFRADYVTEMVRQEMVKRYGEDVAYNKGFKVYATVLSTDQKAAQD
HHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEHHCCHHHHHH
ALSENLINYDRRHGWRGAEKLWSGKEAWDDEKIIDHLSKLPNSDPFIGAAVMAVGKNGYT
HHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHEEECCCCCE
IMLPNGDKEELQKSNAVFGKQIKLNVGEQIWVRKYKNGKDWQLGQIPEVNSALVSLNSDN
EECCCCCHHHHHHHCCCCCCEEEECCCCEEEHHCCCCCCCCCCCCCCCCCCEEEEECCCC
GAIEAIVGGFSFEQSRFNRATQSLVQVGSSIKPFIYAAAMNKGLSLSTTLSDSPITITKK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEHHCCCCEEEEECCCCCEEEEEC
GQKPWTPKNADNRYEGPLRLRVALGKSKNMIAIRALQMAGVDYVAEYLERFGFNRDQFVA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHHHHCCCCHHHHH
TEALALGAASFTPLEMARGYAVFDNGGYLIEPYIIERILDNQGNELFKANPAIACMDCNI
HHHHHHCCCCCCHHHHHCCEEEEECCCEEECHHHHHHHHCCCCCCEEECCCCEEEEECCC
GVKYPEPTYFDGVKIHDETQLQGNSEISENDESNDTSAENDDELEIKPAVKSDAPSLMTE
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHH
AAQNGTETRYAPRVISGELAFLMRSALNSAIYGEPGQAWNGTSKALANEFKRKDIGGKTG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
TTNNSKVTWYAGFGANIVTTVYVGFDDNKRDLGKGEAGARTAMPAWIKYMKVALADKKER
CCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
TPVMPPNIIETKIDSVSGFLGNNLTEYFIKGTEPTKKYIVEKAYKLPQEAKPHLEQRLGL
CCCCCCCHHHHHHHHHHHHHCCCCCHHEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCC
PPKELLNSPGSFELF
CHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1916268; 7542800; 7592463; 10675023 [H]