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The map label for this gene is yfiF [C]

Identifier: 126207693

GI number: 126207693

Start: 223489

End: 224526

Strand: Reverse

Name: yfiF [C]

Synonym: APL_0205

Alternate gene names: 126207693

Gene position: 224526-223489 (Counterclockwise)

Preceding gene: 126207696

Following gene: 126207683

Centisome position: 9.87

GC content: 43.45

Gene sequence:

>1038_bases
ATGAAACCAAAATTTAATGAAAATAAACCTCGCTTTCAAACCGTTGAAGAGCGCCGAGATAACAAATTCCAAACAAAATC
AAACCGATTCGAAGATAAACCTCGTTTTGATAAACCGAAATTCGGCGGCGACAAAGCATGCTTTGATCGTAAAGATCACC
AAGAGGCTCGTGAAGCGATTTACCCGACAATCGGTAAAAAAGCCGCTGGCGAAGGCAACGTACAAATTAGCGTAAAGGGC
GGCAATAACACAATGAAAGAGAAAAAAACCGGCCCGCTTTCTCCTCGCGCACCGGAGAAAATTAAGAAAAATCGTGCGGA
AGAAATGAAAGTGTACGGCGAAAACGCTTGTATTGCATTGTTTAAACAAAGACCGGATGCGATTGTTCGCTTATGGGCAA
CCGTAGAAGGCGCGAAAAAGTTAGGCGATATGCTTAGCTACCTAGCCGAACACAAAAAAGCTTATCATGTGGTAGATCGT
GCCGAAATGGAACGTGTCACCGGCACGGAACACCACGGTGATGTCTGTTTATTAGTCAAAAAAGCTGTGCCGTTCTCATT
AGAAGGCTATTTACAAGTACCGCGTAAACGTGATTGTTTAGTCTTACTGGATGCGGTAAACAATGCACAAAACGTAGGCG
GTATTATTCGCACTTGTGCTTTCTATGGCGTAAACGGTGTGATTGTCGAAAGTGCCGATACGCTTAATTCAAGCGCTGCG
GCACGTGTTGCCGAAGGCGGTTTAGAATTTGTTCGCCCGCTCGAAACGAAAAATAAACAAATCGCACTAACGCAATTACG
TAATGCCGGCTATCAAGTCGTCCATTTAACTCGAAATAAACAAGCTGCGCCACTGGCAAAAACCAAATTAGCTGCAAAAA
CAGTATTCGTATTAAGTGAAATTCCGGCACAAGACATTGAATATCCGGAAGATACCACCGTACAGCTTTCTTTTGAAAAC
CCGTTAAACAGCGGCTTAAATGTGGCGGTAAGCACAGGTATCCTACTTAACCAATGGTATCAAGCGAATATCATCTAA

Upstream 100 bases:

>100_bases
AATAACAAGCGGTCCGATTCGCAAAATTTTTTGCAAAAAGTGATATTTTACTAGGCGAATAAACTATAATAGCCCGAAAT
TTTCCCTAAGGAACAATAGT

Downstream 100 bases:

>100_bases
TCTCGAAACTAAAACAGGCTTACGCAAAAACGTAAGCCTGTTTTACTTTAGATCTTTGTTAGCTTATTAGCCCTTTTATT
CCGTCAAAAATTAAAATCAT

Product: rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKPKFNENKPRFQTVEERRDNKFQTKSNRFEDKPRFDKPKFGGDKACFDRKDHQEAREAIYPTIGKKAAGEGNVQISVKG
GNNTMKEKKTGPLSPRAPEKIKKNRAEEMKVYGENACIALFKQRPDAIVRLWATVEGAKKLGDMLSYLAEHKKAYHVVDR
AEMERVTGTEHHGDVCLLVKKAVPFSLEGYLQVPRKRDCLVLLDAVNNAQNVGGIIRTCAFYGVNGVIVESADTLNSSAA
ARVAEGGLEFVRPLETKNKQIALTQLRNAGYQVVHLTRNKQAAPLAKTKLAAKTVFVLSEIPAQDIEYPEDTTVQLSFEN
PLNSGLNVAVSTGILLNQWYQANII

Sequences:

>Translated_345_residues
MKPKFNENKPRFQTVEERRDNKFQTKSNRFEDKPRFDKPKFGGDKACFDRKDHQEAREAIYPTIGKKAAGEGNVQISVKG
GNNTMKEKKTGPLSPRAPEKIKKNRAEEMKVYGENACIALFKQRPDAIVRLWATVEGAKKLGDMLSYLAEHKKAYHVVDR
AEMERVTGTEHHGDVCLLVKKAVPFSLEGYLQVPRKRDCLVLLDAVNNAQNVGGIIRTCAFYGVNGVIVESADTLNSSAA
ARVAEGGLEFVRPLETKNKQIALTQLRNAGYQVVHLTRNKQAAPLAKTKLAAKTVFVLSEIPAQDIEYPEDTTVQLSFEN
PLNSGLNVAVSTGILLNQWYQANII
>Mature_345_residues
MKPKFNENKPRFQTVEERRDNKFQTKSNRFEDKPRFDKPKFGGDKACFDRKDHQEAREAIYPTIGKKAAGEGNVQISVKG
GNNTMKEKKTGPLSPRAPEKIKKNRAEEMKVYGENACIALFKQRPDAIVRLWATVEGAKKLGDMLSYLAEHKKAYHVVDR
AEMERVTGTEHHGDVCLLVKKAVPFSLEGYLQVPRKRDCLVLLDAVNNAQNVGGIIRTCAFYGVNGVIVESADTLNSSAA
ARVAEGGLEFVRPLETKNKQIALTQLRNAGYQVVHLTRNKQAAPLAKTKLAAKTVFVLSEIPAQDIEYPEDTTVQLSFEN
PLNSGLNVAVSTGILLNQWYQANII

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=277, Percent_Identity=41.1552346570397, Blast_Score=211, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 38440; Mature: 38440

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKFNENKPRFQTVEERRDNKFQTKSNRFEDKPRFDKPKFGGDKACFDRKDHQEAREAI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHH
YPTIGKKAAGEGNVQISVKGGNNTMKEKKTGPLSPRAPEKIKKNRAEEMKVYGENACIAL
CCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHEECCCEEEEE
FKQRPDAIVRLWATVEGAKKLGDMLSYLAEHKKAYHVVDRAEMERVTGTEHHGDVCLLVK
ECCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
KAVPFSLEGYLQVPRKRDCLVLLDAVNNAQNVGGIIRTCAFYGVNGVIVESADTLNSSAA
CCCCCCCCHHEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCHHCCCHHH
ARVAEGGLEFVRPLETKNKQIALTQLRNAGYQVVHLTRNKQAAPLAKTKLAAKTVFVLSE
HHHHHCCHHHHHCCCCCCCEEEHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IPAQDIEYPEDTTVQLSFENPLNSGLNVAVSTGILLNQWYQANII
CCHHHCCCCCCCEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCC
>Mature Secondary Structure
MKPKFNENKPRFQTVEERRDNKFQTKSNRFEDKPRFDKPKFGGDKACFDRKDHQEAREAI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHH
YPTIGKKAAGEGNVQISVKGGNNTMKEKKTGPLSPRAPEKIKKNRAEEMKVYGENACIAL
CCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHEECCCEEEEE
FKQRPDAIVRLWATVEGAKKLGDMLSYLAEHKKAYHVVDRAEMERVTGTEHHGDVCLLVK
ECCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
KAVPFSLEGYLQVPRKRDCLVLLDAVNNAQNVGGIIRTCAFYGVNGVIVESADTLNSSAA
CCCCCCCCHHEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCHHCCCHHH
ARVAEGGLEFVRPLETKNKQIALTQLRNAGYQVVHLTRNKQAAPLAKTKLAAKTVFVLSE
HHHHHCCHHHHHCCCCCCCEEEHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IPAQDIEYPEDTTVQLSFENPLNSGLNVAVSTGILLNQWYQANII
CCHHHCCCCCCCEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]