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The map label for this gene is rnfA

Identifier: 126207653

GI number: 126207653

Start: 183432

End: 184013

Strand: Direct

Name: rnfA

Synonym: APL_0165

Alternate gene names: 126207653

Gene position: 183432-184013 (Clockwise)

Preceding gene: 126207650

Following gene: 126207654

Centisome position: 8.06

GC content: 44.33

Gene sequence:

>582_bases
ATGGTCGATTACATTCTTTTAATTATCAGTACCGCACTCATAAATAACTTTGTCCTAGTAAAATTCTTAGGGCTTTGTCC
GTTTATGGGGGTGTCTAAAAAAGTGGAAACCGCGATTGGTATGGGGATGGCGACCACTTTCGTGCTGACCGTCGCTTCGT
TGTCGGCATATCTCGTCGAAACTTACGTATTAATTCCTTTAGAGGCGCAATTTTTACGTACATTGGTGTTTATTTTAGTT
ATTGCGGTGATTGTGCAATTAACGGAAATGATCGTCCACAAAACCAGTCCGACTTTATACCGTTTGCTCGGTATTTATCT
GCCGTTAATCACGACTAACTGCGCGGTACTCGGTGTTGCGTTATTAAACGTAAATCTTTCCAATAATCTGGTTGAATCGG
TACTTTACGGCTTTGGTGCGGCGTTAGGTTTTTCTTTGGTATTGGTTTTATTTGCCGCATTGCGTGAGCGTTTGGCGGCG
GCGGACGTACCGCGTCCGTTCCAAGGCGCTTCCATCGCTTTAATTACGGCGGGACTCATGTCGCTTGCCTTTATGGGCTT
CACCGGTTTGGTAAAAATCTAA

Upstream 100 bases:

>100_bases
ATAATTTCTAAAGAATAAGCGGTAGGATTTTGCGGAATTCTTGCAAAATTTTCACTAAAAACAACCGCTTATTTTTATTT
TGTCTTTTATTGTGGATACT

Downstream 100 bases:

>100_bases
TGTTAGCATTTCCTATTATTGTGTATATCCTGATTGCGATTGCCGTGATCGCACTGATTTTCGGTGCGATTTTGGGCTAT
TCGTCGATCAAATTAAAAGT

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MVDYILLIISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGMATTFVLTVASLSAYLVETYVLIPLEAQFLRTLVFILV
IAVIVQLTEMIVHKTSPTLYRLLGIYLPLITTNCAVLGVALLNVNLSNNLVESVLYGFGAALGFSLVLVLFAALRERLAA
ADVPRPFQGASIALITAGLMSLAFMGFTGLVKI

Sequences:

>Translated_193_residues
MVDYILLIISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGMATTFVLTVASLSAYLVETYVLIPLEAQFLRTLVFILV
IAVIVQLTEMIVHKTSPTLYRLLGIYLPLITTNCAVLGVALLNVNLSNNLVESVLYGFGAALGFSLVLVLFAALRERLAA
ADVPRPFQGASIALITAGLMSLAFMGFTGLVKI
>Mature_193_residues
MVDYILLIISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGMATTFVLTVASLSAYLVETYVLIPLEAQFLRTLVFILV
IAVIVQLTEMIVHKTSPTLYRLLGIYLPLITTNCAVLGVALLNVNLSNNLVESVLYGFGAALGFSLVLVLFAALRERLAA
ADVPRPFQGASIALITAGLMSLAFMGFTGLVKI

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=75.1295336787565, Blast_Score=300, Evalue=3e-83,
Organism=Escherichia coli, GI1787919, Length=142, Percent_Identity=34.5070422535211, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFA_ACTP2 (A3MYN7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052878.1
- STRING:   A3MYN7
- GeneID:   4849320
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0165
- eggNOG:   COG4657
- HOGENOM:   HBG624915
- OMA:   LRTIAFI
- PhylomeDB:   A3MYN7
- ProtClustDB:   PRK05151
- BioCyc:   APLE416269:APL_0165-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00459
- InterPro:   IPR003667
- InterPro:   IPR011293
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01943

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 20719; Mature: 20719

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ad91454)-; HASH(0x1b0ed3e8)-; HASH(0x1b3e6044)-; HASH(0x1b8a7778)-; HASH(0x1bca7c1c)-; HASH(0x1c25f6fc)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDYILLIISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGMATTFVLTVASLSAYLVE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYVLIPLEAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRLLGIYLPLITTNCAVLGVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNLSNNLVESVLYGFGAALGFSLVLVLFAALRERLAAADVPRPFQGASIALITAGLM
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVKI
HHHHHHHHHHHCH
>Mature Secondary Structure
MVDYILLIISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGMATTFVLTVASLSAYLVE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYVLIPLEAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRLLGIYLPLITTNCAVLGVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNLSNNLVESVLYGFGAALGFSLVLVLFAALRERLAAADVPRPFQGASIALITAGLM
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVKI
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA