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The map label for this gene is apbE [H]

Identifier: 126207644

GI number: 126207644

Start: 174116

End: 175147

Strand: Direct

Name: apbE [H]

Synonym: APL_0156

Alternate gene names: 126207644

Gene position: 174116-175147 (Clockwise)

Preceding gene: 126207643

Following gene: 126207645

Centisome position: 7.66

GC content: 39.83

Gene sequence:

>1032_bases
TTGAAACTTAAATCTATTTTAATTTTTGCAATATCGGCAATTTTTTTAACCGCTTGTAACAAAGCTCCGGAGCAAATTAC
CTTAAAAGGTAAAACGATGGGTACCACTTATACGGTTAAATATATTGATAACGGTGAGTTACAAAACCTTCAAAAACCTG
AGGAAGTCCAAAAAACATTAGATGATTTGCTTAAGCAAGTTAATAATGAAATGTCCACTTATCAGCCGGATTCGCAAATC
AGCCGATTTAATGCGATGCAAGAGGTAAATAAGGCAGTGCAAATTTCGCCTGATTTTGCTAAGGTTGTGCGTGAAGCTAT
TCGGTTAAATCGGGTAACTGAAGGTGCGTTGGATGTTACGGTTGGTAAGCTGGTCAATTTATGGGGATTCGGTCCGGATA
AACGCTTAAATAAAGCACCAAGTGCAGAACAGATTGCTGCTCTTGCACCGAGTGTAGGGATTGAGAAATTAAGTGTAAGT
GATAACTCACTGATGAAATCAGTGCCTAATTTATATTTAGATCTGTCTTCTATTGCGAAAGGTTTTGGCGTAGATAAATT
AGCGGAACATCTCGAAAGCCTAGGTTTAGCCAATTACTTAGTGGAAATCGGCGGCGAGTTGCGTGGTAAAGGGAAAAATT
TACAAGGTGTCGATTGGCGTATTGCGATTGAGCAACCGACTTTAGCACAAGGGCAAGCGGCACAAATTACCGTGCCGTTA
CATAATTTGGGTATGGCGACTTCCGGTAATTACCGCAATTATTTTGAAGACGAGCAAGGTAACCGTCTTTCACATATTAT
TAATCCGAAAGAATTACGTCCGGTTAGCCATAAATTAGCTTCGATTACCGTATTTGCCCCGACCACAATGACGGCAGATG
GTTTATCAACCGGCTTGTTCGTATTAGGGCCGGAAAAAGCGCTGGAAGTGGCTGAACGTGAAAAGTTAGCAGTATTTCTG
ATTATCAAGAACGGCGAGGCATTTGAAACTAAAATGTCTCGTGAATTTGAAAAATTAATTAACCAAAAATAA

Upstream 100 bases:

>100_bases
GGTTTGTTTTTACAGGCTTTTTCAATAGTAATTTTAATTTATAAGGGCGGGCAGATTGACTTGTTTAAAAATCTTACGCA
CTAAAATAAAGAGGTTTAAT

Downstream 100 bases:

>100_bases
TTGGAGTAAACAATGGAAACATTATTAATTACTTTCGGCTTTTTTATTGCCGTAATTTTTGCGATGTCGATTGGTTTTAT
CGTAAAAGGAAAAACAATCA

Product: thiamine biosynthesis lipoprotein ApbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKLKSILIFAISAIFLTACNKAPEQITLKGKTMGTTYTVKYIDNGELQNLQKPEEVQKTLDDLLKQVNNEMSTYQPDSQI
SRFNAMQEVNKAVQISPDFAKVVREAIRLNRVTEGALDVTVGKLVNLWGFGPDKRLNKAPSAEQIAALAPSVGIEKLSVS
DNSLMKSVPNLYLDLSSIAKGFGVDKLAEHLESLGLANYLVEIGGELRGKGKNLQGVDWRIAIEQPTLAQGQAAQITVPL
HNLGMATSGNYRNYFEDEQGNRLSHIINPKELRPVSHKLASITVFAPTTMTADGLSTGLFVLGPEKALEVAEREKLAVFL
IIKNGEAFETKMSREFEKLINQK

Sequences:

>Translated_343_residues
MKLKSILIFAISAIFLTACNKAPEQITLKGKTMGTTYTVKYIDNGELQNLQKPEEVQKTLDDLLKQVNNEMSTYQPDSQI
SRFNAMQEVNKAVQISPDFAKVVREAIRLNRVTEGALDVTVGKLVNLWGFGPDKRLNKAPSAEQIAALAPSVGIEKLSVS
DNSLMKSVPNLYLDLSSIAKGFGVDKLAEHLESLGLANYLVEIGGELRGKGKNLQGVDWRIAIEQPTLAQGQAAQITVPL
HNLGMATSGNYRNYFEDEQGNRLSHIINPKELRPVSHKLASITVFAPTTMTADGLSTGLFVLGPEKALEVAEREKLAVFL
IIKNGEAFETKMSREFEKLINQK
>Mature_343_residues
MKLKSILIFAISAIFLTACNKAPEQITLKGKTMGTTYTVKYIDNGELQNLQKPEEVQKTLDDLLKQVNNEMSTYQPDSQI
SRFNAMQEVNKAVQISPDFAKVVREAIRLNRVTEGALDVTVGKLVNLWGFGPDKRLNKAPSAEQIAALAPSVGIEKLSVS
DNSLMKSVPNLYLDLSSIAKGFGVDKLAEHLESLGLANYLVEIGGELRGKGKNLQGVDWRIAIEQPTLAQGQAAQITVPL
HNLGMATSGNYRNYFEDEQGNRLSHIINPKELRPVSHKLASITVFAPTTMTADGLSTGLFVLGPEKALEVAEREKLAVFL
IIKNGEAFETKMSREFEKLINQK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=343, Percent_Identity=41.6909620991254, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37727; Mature: 37727

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKSILIFAISAIFLTACNKAPEQITLKGKTMGTTYTVKYIDNGELQNLQKPEEVQKTL
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
DDLLKQVNNEMSTYQPDSQISRFNAMQEVNKAVQISPDFAKVVREAIRLNRVTEGALDVT
HHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCHHHHH
VGKLVNLWGFGPDKRLNKAPSAEQIAALAPSVGIEKLSVSDNSLMKSVPNLYLDLSSIAK
HHHHHHHHCCCCCHHCCCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHEEEHHHHHC
GFGVDKLAEHLESLGLANYLVEIGGELRGKGKNLQGVDWRIAIEQPTLAQGQAAQITVPL
CCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEH
HNLGMATSGNYRNYFEDEQGNRLSHIINPKELRPVSHKLASITVFAPTTMTADGLSTGLF
HHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCHHHHHHEEEEEEECCCEECCCCCCCEE
VLGPEKALEVAEREKLAVFLIIKNGEAFETKMSREFEKLINQK
EECCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLKSILIFAISAIFLTACNKAPEQITLKGKTMGTTYTVKYIDNGELQNLQKPEEVQKTL
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
DDLLKQVNNEMSTYQPDSQISRFNAMQEVNKAVQISPDFAKVVREAIRLNRVTEGALDVT
HHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCHHHHH
VGKLVNLWGFGPDKRLNKAPSAEQIAALAPSVGIEKLSVSDNSLMKSVPNLYLDLSSIAK
HHHHHHHHCCCCCHHCCCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHEEEHHHHHC
GFGVDKLAEHLESLGLANYLVEIGGELRGKGKNLQGVDWRIAIEQPTLAQGQAAQITVPL
CCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEH
HNLGMATSGNYRNYFEDEQGNRLSHIINPKELRPVSHKLASITVFAPTTMTADGLSTGLF
HHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCHHHHHHEEEEEEECCCEECCCCCCCEE
VLGPEKALEVAEREKLAVFLIIKNGEAFETKMSREFEKLINQK
EECCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]