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The map label for this gene is nqrF [H]

Identifier: 126207643

GI number: 126207643

Start: 172680

End: 173909

Strand: Direct

Name: nqrF [H]

Synonym: APL_0155

Alternate gene names: 126207643

Gene position: 172680-173909 (Clockwise)

Preceding gene: 126207642

Following gene: 126207644

Centisome position: 7.59

GC content: 41.46

Gene sequence:

>1230_bases
GTGGATAGTAATTTTATTTTCGGTATTATCGCATTTACTGCTCTGGTATTAGTGCTTGCGGTGATCATTCTTTTCGCTAA
ATCAAAATTAGTCGATTCAGGTGATATTACCATCTCGATCAACAACGATCCGGAAAAAGGCATTACGCTTCCGGCGGGTG
GTAAATTACTTGGTGCTTTAGCGAGCAAAGGTATTTTCGTATCGTCAGCTTGCGGCGGCGGCGGCTCATGCGGTCAATGT
AAAGTACAAGTAAAATCCGGCGGCGGTGAAATTCTTCCAACCGAGTTATCGCACATTTCTAAGAAAGAAGCGAAAGAAGG
TTGGCGTTTAGCGTGTCAGGTAAACGTGAAATCTTCAATGGATGTTGAACTTCCGGAAGAAATCTTTGGTGTGAAAAAAT
GGGAATGTACCGTTATTTCTAACGATAACAAAGCAACCTTCATCAAAGAGCTTAAACTTCAAATTCCTGAAGGCGAAGAA
GTACCTTTCCGTGCGGGTGGTTATATCCAAATTGAAGCGGATCCGCATACGGTTAATTATAAAGACTTCGATATTCCGAA
AGAATATCACGAAGACTGGGATAAATTTAACTTATGGCGTTATGTATCAAAAGTGGACGAGCATATTATTCGTGCTTATT
CAATGGCTTCATATCCGGAAGAGAAAGGCATTATTATGTTAAATGTGCGTATTGCAACGCCTCCTCCACGTAACCCAGAT
GTTCCACCGGGTCAAATGTCTTCATACATTTGGTCATTAAAACCGGGTGATAAAGTAACGATTTCCGGTCCGTTCGGTGA
ATTCTTCGCGAAAGAAACCGACAACGAAATGGTGTTTATCGGTGGTGGTGCAGGTATGGCGCCAATGCGTTCGCATATCT
TTGACCAATTAAAACGTTTAAAATCTAAACGTAAAATGTCATTCTGGTATGGTGCTCGTTCTAAACGTGAAATCTTCTAC
CAAGAAGACTTCGACCAATTAGCGGCCGAAAACGATAACTTCGTATGGCACGTAGCACTTTCAGATGCGTTACCGGAAGA
TAACTGGACAGGTTATACAGGCTTTATTCACAATGTACTTTATGAAAACTATCTGAAAAATCATGAAGCACCGGAAGACT
GTGAATACTATATGTGTGGTCCACCGGTGATGAATGCAGCGGTAATCGGTATGCTGAAGAGCTTAGGTGTTGAAGACGAA
AACATCTTATTAGATGATTTCGGTGGTTAA

Upstream 100 bases:

>100_bases
CCGGCTGGTTTACGTGGTTTAGGTATTACCTTTATCACTGTAGGTTTAATGGCGTTAGGTTTCATGTCATTCTCGGGCAT
TCAATTATAAGGAGCATATC

Downstream 100 bases:

>100_bases
TACCAAATAGAACAGAGGGTAAATGTACCCTGATTTACAAGCGGTGTGATTTGCAAAGTCTTTTGTAAATCACACCGCTT
GAAGTGCTTTAAATTATTAG

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDPEKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQC
KVQVKSGGGEILPTELSHISKKEAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
VPFRAGGYIQIEADPHTVNYKDFDIPKEYHEDWDKFNLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRNPD
VPPGQMSSYIWSLKPGDKVTISGPFGEFFAKETDNEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSKREIFY
QEDFDQLAAENDNFVWHVALSDALPEDNWTGYTGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGMLKSLGVEDE
NILLDDFGG

Sequences:

>Translated_409_residues
MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDPEKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQC
KVQVKSGGGEILPTELSHISKKEAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
VPFRAGGYIQIEADPHTVNYKDFDIPKEYHEDWDKFNLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRNPD
VPPGQMSSYIWSLKPGDKVTISGPFGEFFAKETDNEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSKREIFY
QEDFDQLAAENDNFVWHVALSDALPEDNWTGYTGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGMLKSLGVEDE
NILLDDFGG
>Mature_409_residues
MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDPEKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQC
KVQVKSGGGEILPTELSHISKKEAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
VPFRAGGYIQIEADPHTVNYKDFDIPKEYHEDWDKFNLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRNPD
VPPGQMSSYIWSLKPGDKVTISGPFGEFFAKETDNEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSKREIFY
QEDFDQLAAENDNFVWHVALSDALPEDNWTGYTGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGMLKSLGVEDE
NILLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17563926, Length=220, Percent_Identity=28.1818181818182, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323552, Length=218, Percent_Identity=25.6880733944954, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007804, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007806, Length=218, Percent_Identity=27.5229357798165, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 45509; Mature: 45509

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDPEKGITLPAGGKLLGAL
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEECCCCCHHHHHH
ASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKKEAKEGWRLACQVNVKSSM
HCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
DVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEEVPFRAGGYIQIEADPHTVNY
CCCCCHHHHCCCEEEEEEEECCCCEEEEEEHEEECCCCCCCCCCCCCEEEEECCCCEECC
KDFDIPKEYHEDWDKFNLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRNPD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCC
VPPGQMSSYIWSLKPGDKVTISGPFGEFFAKETDNEMVFIGGGAGMAPMRSHIFDQLKRL
CCCCCHHHEEEEECCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
KSKRKMSFWYGARSKREIFYQEDFDQLAAENDNFVWHVALSDALPEDNWTGYTGFIHNVL
HHHCCEEEEECCCCCCEEEEHHCHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHH
YENYLKNHEAPEDCEYYMCGPPVMNAAVIGMLKSLGVEDENILLDDFGG
HHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDPEKGITLPAGGKLLGAL
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEECCCCCHHHHHH
ASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKKEAKEGWRLACQVNVKSSM
HCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
DVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEEVPFRAGGYIQIEADPHTVNY
CCCCCHHHHCCCEEEEEEEECCCCEEEEEEHEEECCCCCCCCCCCCCEEEEECCCCEECC
KDFDIPKEYHEDWDKFNLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRNPD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCC
VPPGQMSSYIWSLKPGDKVTISGPFGEFFAKETDNEMVFIGGGAGMAPMRSHIFDQLKRL
CCCCCHHHEEEEECCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
KSKRKMSFWYGARSKREIFYQEDFDQLAAENDNFVWHVALSDALPEDNWTGYTGFIHNVL
HHHCCEEEEECCCCCCEEEEHHCHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHH
YENYLKNHEAPEDCEYYMCGPPVMNAAVIGMLKSLGVEDENILLDDFGG
HHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA