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The map label for this gene is ansB [H]

Identifier: 126207623

GI number: 126207623

Start: 149916

End: 150965

Strand: Reverse

Name: ansB [H]

Synonym: APL_0135

Alternate gene names: 126207623

Gene position: 150965-149916 (Counterclockwise)

Preceding gene: 126207626

Following gene: 126207618

Centisome position: 6.64

GC content: 45.52

Gene sequence:

>1050_bases
ATGAAACTCAAAAAACTTGCTTTATCTGCTGTTCTTTCTATGGCAATTTCGAGTGTGTATGCCGCGGAGTTACCGAATAT
CACAATATTGGCAACCGGCGGAACGATTGCCGGCAGCGGACAAAGTGCGGTGAGTTCGGCTTACCAAGCAGGACAATTGA
ACATTGATACCTTAATTGAAGCCGTACCGGAAATGAAGACGTTGGCAAACATTAAAGGTGAACAAGTAGTCAAAATCGGT
TCACAAGATATGAGTGATGAGGTATGGCTAAAATTAGCGAAAACCGTTAATAGTCAGTGCGCTAATACGGACGGTTTTGT
CATTACTCACGGCACGGATACGATGGAAGAAACCGCTTATTTCCTCGATATGACGGTAAAATGCGATAAACCGGTGGTAC
TGGTCGGCGCAATGCGTCCGGCAACGGAGAAAAGTGCGGACGGCCCGCTGAATTTATATAATTCAATTGTAGTAGCGAAA
GATAAAAAATCGGCAAAACGTGGTGTGTTAGTCGCAATGAATGATGTGGTACTGGGTGCGAGAGACGTGACCAAAACCAG
TACCACCGCCGTACAAACATTCAGTTCGCCGAACTTCGGTACGCTCGGCTATATTCATAACAGCAAAGTCGATTACGAAC
GTGCGCCGGAAAGTAAACATACGTTAAATACCCCGTTTACGATTGATAACCTCAATGAACTGCCGAAAGTCGGTATTATC
TACGCTTATTCAAATATGCCGACCGAGCCGCTTAAAGCCTTATTGGATGCGGGCTATCAAGGGATTGTGGTTGCCGGTGT
CGGTAACGGTAATATGAATGCGGCGAATTTAGCGTTACTGGAACAAGCGGCTAAAAACGGTGTTGCGGTGGTTCGTTCTT
CACGAGTGCCGACCGGCTATACCACTCGTGATGCGGAAGTGGACGATTCTAAATACGGTTTTGCGGCATCCGGTACGCTC
AACCCGCAAAAAGCACGTGTATTATTACAACTTGCCTTAACCCAAACCAAAGACATTAATACGATTCAACAATATTTTGA
AGACTTCTAG

Upstream 100 bases:

>100_bases
ATTTTGTGAACAAGATCAAAGAATAAGAGGCAAGTGTTTCTATCCGTTCTGCGTTCCATGGGATAATTATTTTATCTTTC
TCAAATCTTAAGGAGCTTAT

Downstream 100 bases:

>100_bases
TTTTCAGCCATAAAAGCAAGCGGTCTATTTCTTGCAAAAAATTGCGAAAAATAGACCGCTTGTCTTTTTGTAACCGTCGC
CTATTTTACAAAGCTATCAA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKLKKLALSAVLSMAISSVYAAELPNITILATGGTIAGSGQSAVSSAYQAGQLNIDTLIEAVPEMKTLANIKGEQVVKIG
SQDMSDEVWLKLAKTVNSQCANTDGFVITHGTDTMEETAYFLDMTVKCDKPVVLVGAMRPATEKSADGPLNLYNSIVVAK
DKKSAKRGVLVAMNDVVLGARDVTKTSTTAVQTFSSPNFGTLGYIHNSKVDYERAPESKHTLNTPFTIDNLNELPKVGII
YAYSNMPTEPLKALLDAGYQGIVVAGVGNGNMNAANLALLEQAAKNGVAVVRSSRVPTGYTTRDAEVDDSKYGFAASGTL
NPQKARVLLQLALTQTKDINTIQQYFEDF

Sequences:

>Translated_349_residues
MKLKKLALSAVLSMAISSVYAAELPNITILATGGTIAGSGQSAVSSAYQAGQLNIDTLIEAVPEMKTLANIKGEQVVKIG
SQDMSDEVWLKLAKTVNSQCANTDGFVITHGTDTMEETAYFLDMTVKCDKPVVLVGAMRPATEKSADGPLNLYNSIVVAK
DKKSAKRGVLVAMNDVVLGARDVTKTSTTAVQTFSSPNFGTLGYIHNSKVDYERAPESKHTLNTPFTIDNLNELPKVGII
YAYSNMPTEPLKALLDAGYQGIVVAGVGNGNMNAANLALLEQAAKNGVAVVRSSRVPTGYTTRDAEVDDSKYGFAASGTL
NPQKARVLLQLALTQTKDINTIQQYFEDF
>Mature_349_residues
MKLKKLALSAVLSMAISSVYAAELPNITILATGGTIAGSGQSAVSSAYQAGQLNIDTLIEAVPEMKTLANIKGEQVVKIG
SQDMSDEVWLKLAKTVNSQCANTDGFVITHGTDTMEETAYFLDMTVKCDKPVVLVGAMRPATEKSADGPLNLYNSIVVAK
DKKSAKRGVLVAMNDVVLGARDVTKTSTTAVQTFSSPNFGTLGYIHNSKVDYERAPESKHTLNTPFTIDNLNELPKVGII
YAYSNMPTEPLKALLDAGYQGIVVAGVGNGNMNAANLALLEQAAKNGVAVVRSSRVPTGYTTRDAEVDDSKYGFAASGTL
NPQKARVLLQLALTQTKDINTIQQYFEDF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=230, Percent_Identity=27.8260869565217, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789327, Length=347, Percent_Identity=68.0115273775216, Blast_Score=456, Evalue=1e-130,
Organism=Escherichia coli, GI1788065, Length=289, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI193207372, Length=226, Percent_Identity=29.2035398230088, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558280, Length=226, Percent_Identity=29.2035398230088, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193207374, Length=226, Percent_Identity=29.2035398230088, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558278, Length=226, Percent_Identity=29.2035398230088, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=333, Percent_Identity=39.6396396396396, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323187, Length=333, Percent_Identity=39.6396396396396, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323186, Length=333, Percent_Identity=39.6396396396396, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323184, Length=333, Percent_Identity=39.6396396396396, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320527, Length=336, Percent_Identity=35.7142857142857, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24639609, Length=230, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645530, Length=220, Percent_Identity=28.6363636363636, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37210; Mature: 37210

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLALSAVLSMAISSVYAAELPNITILATGGTIAGSGQSAVSSAYQAGQLNIDTLIE
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHHHHCCCCCHHHHHH
AVPEMKTLANIKGEQVVKIGSQDMSDEVWLKLAKTVNSQCANTDGFVITHGTDTMEETAY
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHEE
FLDMTVKCDKPVVLVGAMRPATEKSADGPLNLYNSIVVAKDKKSAKRGVLVAMNDVVLGA
EEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCEEEEECCEEECC
RDVTKTSTTAVQTFSSPNFGTLGYIHNSKVDYERAPESKHTLNTPFTIDNLNELPKVGII
CCCCCCCHHEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCHHCCCCEEEE
YAYSNMPTEPLKALLDAGYQGIVVAGVGNGNMNAANLALLEQAAKNGVAVVRSSRVPTGY
EEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCC
TTRDAEVDDSKYGFAASGTLNPQKARVLLQLALTQTKDINTIQQYFEDF
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLKKLALSAVLSMAISSVYAAELPNITILATGGTIAGSGQSAVSSAYQAGQLNIDTLIE
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHHHHCCCCCHHHHHH
AVPEMKTLANIKGEQVVKIGSQDMSDEVWLKLAKTVNSQCANTDGFVITHGTDTMEETAY
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHEE
FLDMTVKCDKPVVLVGAMRPATEKSADGPLNLYNSIVVAKDKKSAKRGVLVAMNDVVLGA
EEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCEEEEECCEEECC
RDVTKTSTTAVQTFSSPNFGTLGYIHNSKVDYERAPESKHTLNTPFTIDNLNELPKVGII
CCCCCCCHHEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCHHCCCCEEEE
YAYSNMPTEPLKALLDAGYQGIVVAGVGNGNMNAANLALLEQAAKNGVAVVRSSRVPTGY
EEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCC
TTRDAEVDDSKYGFAASGTLNPQKARVLLQLALTQTKDINTIQQYFEDF
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]