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The map label for this gene is rnhB

Identifier: 126207617

GI number: 126207617

Start: 144477

End: 145070

Strand: Reverse

Name: rnhB

Synonym: APL_0129

Alternate gene names: 126207617

Gene position: 145070-144477 (Counterclockwise)

Preceding gene: 126207618

Following gene: 126207616

Centisome position: 6.38

GC content: 47.31

Gene sequence:

>594_bases
ATGAGTACAAATTTCATTTATCCTAATGCCCACTTGATCGCAGGTGTGGACGAAGTCGGTAGAGGCCCGTTAGTCGGTGC
GGTGGTCACCGCTGCCGTGATTTTAGCTCCGAATAATCCGATTGAAGGATTAGCCGACTCTAAAAAACTGTCGGAGAAGA
AGCGTTTGCTTTTAGCGGAGGAAATTAAAGCAAAAGCGCTTTGTTGGTCTTTAGGGCGTGCCGAACCGGAAGAAATCGAT
CGGTTGAATATCTTGCACGCAACAATGCTCGCTATGCAGCGGGCGGTTGCCGGACTCAATATTCAGCCCGATTTCGTGTT
GGTGGACGGCAACCGTATTCCAACTTTACCGATGCCGGCACAAGCGGTCATAAAAGGAGATAGTTTAGTTGCCGAGATCA
GCGCAGCTTCCATTTTAGCCAAAGTTGCCCGAGATCAGGAAATGGCTGAATTAGATGTGCAATATCCGGAATACGGCTTT
GCAAAGCATAAAGGCTATCCGACTAAACTACATTTTGAAAAACTGGAACAATTCGGGGCAACCCCTTTTCACCGAAAGAG
CTTCGCCCCGGTGAAGAAAATCTTAGGTTTATAA

Upstream 100 bases:

>100_bases
CGTTTCGAAAACTATCATCGATTAATTGAAAGCCGTGATGAAACCAAAAGTCAGCGTCATTTTAGAACGGAAGAATAAAA
TTAAATAAGAATCAATAGCA

Downstream 100 bases:

>100_bases
ATGCTAGAACTACTACTCGAACCCTTCCAATATAACTATATGCAAAAAGCGATTTTCGTCAGTATGGGCGTTGGTGTGGT
TTGTGCCTTTCTCTCCGCCT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSTNFIYPNAHLIAGVDEVGRGPLVGAVVTAAVILAPNNPIEGLADSKKLSEKKRLLLAEEIKAKALCWSLGRAEPEEID
RLNILHATMLAMQRAVAGLNIQPDFVLVDGNRIPTLPMPAQAVIKGDSLVAEISAASILAKVARDQEMAELDVQYPEYGF
AKHKGYPTKLHFEKLEQFGATPFHRKSFAPVKKILGL

Sequences:

>Translated_197_residues
MSTNFIYPNAHLIAGVDEVGRGPLVGAVVTAAVILAPNNPIEGLADSKKLSEKKRLLLAEEIKAKALCWSLGRAEPEEID
RLNILHATMLAMQRAVAGLNIQPDFVLVDGNRIPTLPMPAQAVIKGDSLVAEISAASILAKVARDQEMAELDVQYPEYGF
AKHKGYPTKLHFEKLEQFGATPFHRKSFAPVKKILGL
>Mature_196_residues
STNFIYPNAHLIAGVDEVGRGPLVGAVVTAAVILAPNNPIEGLADSKKLSEKKRLLLAEEIKAKALCWSLGRAEPEEIDR
LNILHATMLAMQRAVAGLNIQPDFVLVDGNRIPTLPMPAQAVIKGDSLVAEISAASILAKVARDQEMAELDVQYPEYGFA
KHKGYPTKLHFEKLEQFGATPFHRKSFAPVKKILGL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=194, Percent_Identity=74.7422680412371, Blast_Score=298, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ACTP2 (A3MYK1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052842.1
- ProteinModelPortal:   A3MYK1
- SMR:   A3MYK1
- STRING:   A3MYK1
- GeneID:   4850382
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0129
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   A3MYK1
- ProtClustDB:   PRK00015
- BioCyc:   APLE416269:APL_0129-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21417; Mature: 21286

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNFIYPNAHLIAGVDEVGRGPLVGAVVTAAVILAPNNPIEGLADSKKLSEKKRLLLAE
CCCCEECCCCCEEECHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHH
EIKAKALCWSLGRAEPEEIDRLNILHATMLAMQRAVAGLNIQPDFVLVDGNRIPTLPMPA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCH
QAVIKGDSLVAEISAASILAKVARDQEMAELDVQYPEYGFAKHKGYPTKLHFEKLEQFGA
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHCC
TPFHRKSFAPVKKILGL
CCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
STNFIYPNAHLIAGVDEVGRGPLVGAVVTAAVILAPNNPIEGLADSKKLSEKKRLLLAE
CCCEECCCCCEEECHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHH
EIKAKALCWSLGRAEPEEIDRLNILHATMLAMQRAVAGLNIQPDFVLVDGNRIPTLPMPA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCH
QAVIKGDSLVAEISAASILAKVARDQEMAELDVQYPEYGFAKHKGYPTKLHFEKLEQFGA
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHCC
TPFHRKSFAPVKKILGL
CCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA