Click here to switch to the map view.

The map label for this gene is putP [H]

Identifier: 126207595

GI number: 126207595

Start: 125274

End: 126788

Strand: Reverse

Name: putP [H]

Synonym: APL_0107

Alternate gene names: 126207595

Gene position: 126788-125274 (Counterclockwise)

Preceding gene: 126207596

Following gene: 126207594

Centisome position: 5.57

GC content: 45.35

Gene sequence:

>1515_bases
ATGTTCAACTTCGATCCGACAACCATTACATTCATAATTTATATTATTGGAATGATTGCAATCGGCTTTATCGCCTATCG
TGTTACAAATAATTTATCCGACTATATCCTCGGCGGTCGTCGTTTAGGCAGCTTCGTCACCGCCCTATCCGCCGGCGCAT
CGGATATGTCCGGTTGGTTATTAATGGGGCTACCCGGTGCGGTTTATTCAAGCGGTTTAATTGAAGGCTGGTTAGCTGTC
GGGCTTACTATTGGGGCTTATCTTAACTGGAAACTGGTCGCCGGTCGTTTACGTTCACACACCGAACATAGCGGCGATGC
GCTTACGTTGCCCGAATATTTTCATAACCGTTTTAATGACGAAACGCGTATTATCAAAATTTTATCCGCAATCATTTTTT
TACTTTTCTTTGCCGTATATTGCGCTTCGGGCGTAGTTGCCGGTGCTCGCGTATTTGAAAACCTTTTCGGCGTACCTTAT
GAAAATGCGATTTGGTACGGTGCGGTCGCAACCATTCTTTACACCTTTATCGGTGGTTTCTTAGCGGTAAGTTGGACCGA
TACCATTCAAGCTTCGCTAATGTTATTCGCTTTAATTTTAACGCCCGTTTTCGTCATCATAAACGTAGGCGGTTTTGAGG
TGATGCAAGAAACGATTGCGAAAGCCGGCGAATTAGCGCAAAAAGATTATAACGACATTTTTGCTGGTACGACCGTACTC
GGCGTATTAAGCCTACTGGCTTGGGGTTTAGGCTACTTCGGGCAACCGCATATCGTCGTACGTTTTATGGCGGCGGAAAG
CGTAAAATCGCTTGAGAAAGCTCGCCGTATCAGTATGACGTGGATGATTATCTGTTTAGCGGGTGCTATCGGCATCGGTT
ATTTCGGTATGGCATATTTCTTTACTCATCCGGAACAAGCTACCGTCGTTAATCAAAATAAAGAACAAATCTTTATTGAA
TTGGCAAAATTACTGTTTAACCCGTGGATTGCAGGCGTCTTACTGTCCGCTATTTTAGCGGCGGTAATGAGTACCCTCTC
GGCACAGTTATTAATTTGTTCGAGCGCGATTACCGAAGATCTCTATAAAGGTATCCTTCGCCCGTCCGCAACCGATAAAG
AATTAGTATGGCTCGGTCGCATTATGGTGTTAGCGGTTGCCGCATTAGCAATCTATATCGCACAAGATCCGAACAGCCAA
GTATTCGGCTTAGTAAAAGACGCTTGGGCGGGCTTCGGTAGTGCTTTCGGTCCGGTGGTATTACTGTCATTATTCTGGAA
ACGTATGAACGGCTTCGGGGCAATTAGCGGTATGTTAGTAGGAGCGTTAATCGTGTACTTCTGGAAAGACATTACCCTCT
GGGCTAATTTACCGGAAGTCTATTCAATGATTCCGGGCTTCATTCTAGCGACGTTAGCGATTGTCTTGGTTTCGCTCATT
ACTCCGCCGCCGAGCAAAAAGGTAACGGACACATTTGAGCGTGCGGATAAAGCCTATTTAGCAGAGCTTCAATAA

Upstream 100 bases:

>100_bases
TTTGTTAAAAATTCATTTCCATCCTATCTTTTGCTATAATTCACGACTTTTGCATAGCGTTAATTAAATTAGCAACATAG
AAAGAATAAGAGGAATAACT

Downstream 100 bases:

>100_bases
AGACAAAGCGATTTGCCGGCAACGGCAAGTCGCTTTTTTGTTTTCGAAATGCAAACTTTTTCATAAAAACGACCGCTTGT
TAAAGCGAAACAGGAGTCCT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFNFDPTTITFIIYIIGMIAIGFIAYRVTNNLSDYILGGRRLGSFVTALSAGASDMSGWLLMGLPGAVYSSGLIEGWLAV
GLTIGAYLNWKLVAGRLRSHTEHSGDALTLPEYFHNRFNDETRIIKILSAIIFLLFFAVYCASGVVAGARVFENLFGVPY
ENAIWYGAVATILYTFIGGFLAVSWTDTIQASLMLFALILTPVFVIINVGGFEVMQETIAKAGELAQKDYNDIFAGTTVL
GVLSLLAWGLGYFGQPHIVVRFMAAESVKSLEKARRISMTWMIICLAGAIGIGYFGMAYFFTHPEQATVVNQNKEQIFIE
LAKLLFNPWIAGVLLSAILAAVMSTLSAQLLICSSAITEDLYKGILRPSATDKELVWLGRIMVLAVAALAIYIAQDPNSQ
VFGLVKDAWAGFGSAFGPVVLLSLFWKRMNGFGAISGMLVGALIVYFWKDITLWANLPEVYSMIPGFILATLAIVLVSLI
TPPPSKKVTDTFERADKAYLAELQ

Sequences:

>Translated_504_residues
MFNFDPTTITFIIYIIGMIAIGFIAYRVTNNLSDYILGGRRLGSFVTALSAGASDMSGWLLMGLPGAVYSSGLIEGWLAV
GLTIGAYLNWKLVAGRLRSHTEHSGDALTLPEYFHNRFNDETRIIKILSAIIFLLFFAVYCASGVVAGARVFENLFGVPY
ENAIWYGAVATILYTFIGGFLAVSWTDTIQASLMLFALILTPVFVIINVGGFEVMQETIAKAGELAQKDYNDIFAGTTVL
GVLSLLAWGLGYFGQPHIVVRFMAAESVKSLEKARRISMTWMIICLAGAIGIGYFGMAYFFTHPEQATVVNQNKEQIFIE
LAKLLFNPWIAGVLLSAILAAVMSTLSAQLLICSSAITEDLYKGILRPSATDKELVWLGRIMVLAVAALAIYIAQDPNSQ
VFGLVKDAWAGFGSAFGPVVLLSLFWKRMNGFGAISGMLVGALIVYFWKDITLWANLPEVYSMIPGFILATLAIVLVSLI
TPPPSKKVTDTFERADKAYLAELQ
>Mature_504_residues
MFNFDPTTITFIIYIIGMIAIGFIAYRVTNNLSDYILGGRRLGSFVTALSAGASDMSGWLLMGLPGAVYSSGLIEGWLAV
GLTIGAYLNWKLVAGRLRSHTEHSGDALTLPEYFHNRFNDETRIIKILSAIIFLLFFAVYCASGVVAGARVFENLFGVPY
ENAIWYGAVATILYTFIGGFLAVSWTDTIQASLMLFALILTPVFVIINVGGFEVMQETIAKAGELAQKDYNDIFAGTTVL
GVLSLLAWGLGYFGQPHIVVRFMAAESVKSLEKARRISMTWMIICLAGAIGIGYFGMAYFFTHPEQATVVNQNKEQIFIE
LAKLLFNPWIAGVLLSAILAAVMSTLSAQLLICSSAITEDLYKGILRPSATDKELVWLGRIMVLAVAALAIYIAQDPNSQ
VFGLVKDAWAGFGSAFGPVVLLSLFWKRMNGFGAISGMLVGALIVYFWKDITLWANLPEVYSMIPGFILATLAIVLVSLI
TPPPSKKVTDTFERADKAYLAELQ

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=499, Percent_Identity=45.2905811623246, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI110835708, Length=537, Percent_Identity=24.3947858472998, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI14140236, Length=487, Percent_Identity=25.0513347022587, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI4507031, Length=465, Percent_Identity=27.0967741935484, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI167466278, Length=446, Percent_Identity=22.6457399103139, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI109659839, Length=450, Percent_Identity=22.2222222222222, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI109659836, Length=437, Percent_Identity=23.5697940503432, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI4507035, Length=419, Percent_Identity=23.8663484486874, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI157671931, Length=440, Percent_Identity=22.0454545454545, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1787251, Length=494, Percent_Identity=63.1578947368421, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI87082237, Length=451, Percent_Identity=27.0509977827051, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1790503, Length=437, Percent_Identity=24.9427917620137, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790113, Length=492, Percent_Identity=22.3577235772358, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533094, Length=377, Percent_Identity=27.3209549071618, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17539284, Length=421, Percent_Identity=21.6152019002375, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533096, Length=241, Percent_Identity=29.045643153527, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221459584, Length=382, Percent_Identity=24.3455497382199, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645928, Length=380, Percent_Identity=25, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI221459588, Length=429, Percent_Identity=23.7762237762238, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221459582, Length=370, Percent_Identity=23.2432432432432, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650192, Length=365, Percent_Identity=24.3835616438356, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24651741, Length=441, Percent_Identity=24.9433106575964, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640370, Length=354, Percent_Identity=23.4463276836158, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221459586, Length=431, Percent_Identity=20.6496519721578, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19920916, Length=407, Percent_Identity=24.3243243243243, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28573698, Length=473, Percent_Identity=24.5243128964059, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281362918, Length=449, Percent_Identity=24.2761692650334, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 54998; Mature: 54998

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]