Click here to switch to the map view.

The map label for this gene is nrfA [H]

Identifier: 126207588

GI number: 126207588

Start: 106390

End: 107871

Strand: Direct

Name: nrfA [H]

Synonym: APL_0100

Alternate gene names: 126207588

Gene position: 106390-107871 (Clockwise)

Preceding gene: 126207580

Following gene: 126207589

Centisome position: 4.68

GC content: 42.04

Gene sequence:

>1482_bases
ATGGCAACCATTGCAATGTTGGGATGTTTATCATCAGCAGCATGGGCGGAAGAAAAACCGGCTCAAACACCATCTTTAAC
CGATAAAGCTTTAAAACATGAAAAACTAGGAGTGGTAGTAGAATCAGCAAACCATAAATTTGCTGAAAAATATCCGCTTC
AATATAACTCATGGAAAGCAACTTCAGAATCAACCGACCGTGGTAGTGCTTTAGCGGCGGATCCTCGCTACGTTGTGTTA
TGGGCGGGTTATGCATTTGCAAAAGACTATAACAAACCTCGTGGTCACTATTATGCTATAACAGATGTACGTGAAATTTT
ACGTACTGGTGCGCCGAAAGATGAGAATGATGGTCCACAGCCAATGGCTTGTTGGACATGTAAAGGTCCTGATGTTGCAC
GTTTAATCGAAGAAAAAGGTGAACGTGGTTATTTTGATCCTAAATGGGCGAAATACGGTGCGGAAATCGTAAACTCAATC
GGTTGTGCAGACTGTCATGACACAACATCGAAAGAGTTTGAAGAAGGCAAACCTGCATTACGTGTTGCTCGTCCACATGT
ATTACGTGCGTTAGAAACTGTAGGTTGGAAATTTGAAGACTTAGATAAACATGGCAAACGTACCGCAGTATGTGCAAACT
GCCACGTAGAGTACTACTTCAAAGGCAAAACAGACGTAACTTTCCCATGGGATAAAGGTGTAGATGTCGATAGTATCGAA
AAATACTACGATGAAATTCAATTTGCTGACTGGACTCACGCATTATCTAAAGCACCAATGTTAAAAACTCAGCACCCGGA
TTTTGAAATCTGGTCGCAAGGTGTTCATGGTAAAAACGGCGTAACTTGTATTGACTGCCATATGCCGAAAGTGAAAGATA
AAGACGGTAAAGTTTATACGGATCATAAAATCGGTAACCCGTTCGATCAATTTGACGCAACGTGTAAAACTTGTCACGAA
CAAAGCAAAGAAACACTTGAGAAACGTGTGAAAGAACATAAACACCAAGTGAAAGAAGCAATGATTCGTTTAGAAGATCA
ATTAGTGAAAGCGCACTTTGAAGCGAAAGCAGCTTGGGAAGCAGGTGCAACTCAAGAAGAGATGAAAGATATCTTAACTG
CAATTCGTCACGCTCAATGGCGTTGGGATTACTCAGCAGCAGGTCACGGTAGCCATATGCATGCACCTGATGTAATGCTT
CACGTAATCGCATCAGGTATTGACCGTGCAGCGGATGCGCGTGCGCAATTAGGTGTCGTATTAGCGAAACACGGCGTGAC
AACACCGGTAGCAATTCCTGATATCTCTACTAAAGAAAAAGCACAAAAAGCGATTGGTTTAGATATTCCTAAAGATCAAG
CTGCGAAAGATGAGTTCTTACGTACAGTTGTTCCACAATGGGATGCGGAAGCACGTAAAAAAGGCTTATTACCTGCAGAA
GAACCAAAAGCAGTTGTAGCACCAAAAGCAGAAGCGAAATAA

Upstream 100 bases:

>100_bases
CTTAAATGTTTATCTTATTGTTTTTAATAAGAAAAACTCTCATTTCTATCAGGCATTTTGTGGGGGACACAACGTGAAAC
GTTTAACTAAAAACCTAGCA

Downstream 100 bases:

>100_bases
TAGAAGCTTGAGGTGAAATTATGAAAAACATTATCTCAAAAGCAGGGCGAGCAATCGCCCTCCAAGCGGTCGCTTTTGCA
AGTTTTCTTGCAATTTCAAC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MATIAMLGCLSSAAWAEEKPAQTPSLTDKALKHEKLGVVVESANHKFAEKYPLQYNSWKATSESTDRGSALAADPRYVVL
WAGYAFAKDYNKPRGHYYAITDVREILRTGAPKDENDGPQPMACWTCKGPDVARLIEEKGERGYFDPKWAKYGAEIVNSI
GCADCHDTTSKEFEEGKPALRVARPHVLRALETVGWKFEDLDKHGKRTAVCANCHVEYYFKGKTDVTFPWDKGVDVDSIE
KYYDEIQFADWTHALSKAPMLKTQHPDFEIWSQGVHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFDATCKTCHE
QSKETLEKRVKEHKHQVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKDILTAIRHAQWRWDYSAAGHGSHMHAPDVML
HVIASGIDRAADARAQLGVVLAKHGVTTPVAIPDISTKEKAQKAIGLDIPKDQAAKDEFLRTVVPQWDAEARKKGLLPAE
EPKAVVAPKAEAK

Sequences:

>Translated_493_residues
MATIAMLGCLSSAAWAEEKPAQTPSLTDKALKHEKLGVVVESANHKFAEKYPLQYNSWKATSESTDRGSALAADPRYVVL
WAGYAFAKDYNKPRGHYYAITDVREILRTGAPKDENDGPQPMACWTCKGPDVARLIEEKGERGYFDPKWAKYGAEIVNSI
GCADCHDTTSKEFEEGKPALRVARPHVLRALETVGWKFEDLDKHGKRTAVCANCHVEYYFKGKTDVTFPWDKGVDVDSIE
KYYDEIQFADWTHALSKAPMLKTQHPDFEIWSQGVHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFDATCKTCHE
QSKETLEKRVKEHKHQVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKDILTAIRHAQWRWDYSAAGHGSHMHAPDVML
HVIASGIDRAADARAQLGVVLAKHGVTTPVAIPDISTKEKAQKAIGLDIPKDQAAKDEFLRTVVPQWDAEARKKGLLPAE
EPKAVVAPKAEAK
>Mature_492_residues
ATIAMLGCLSSAAWAEEKPAQTPSLTDKALKHEKLGVVVESANHKFAEKYPLQYNSWKATSESTDRGSALAADPRYVVLW
AGYAFAKDYNKPRGHYYAITDVREILRTGAPKDENDGPQPMACWTCKGPDVARLIEEKGERGYFDPKWAKYGAEIVNSIG
CADCHDTTSKEFEEGKPALRVARPHVLRALETVGWKFEDLDKHGKRTAVCANCHVEYYFKGKTDVTFPWDKGVDVDSIEK
YYDEIQFADWTHALSKAPMLKTQHPDFEIWSQGVHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFDATCKTCHEQ
SKETLEKRVKEHKHQVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKDILTAIRHAQWRWDYSAAGHGSHMHAPDVMLH
VIASGIDRAADARAQLGVVLAKHGVTTPVAIPDISTKEKAQKAIGLDIPKDQAAKDEFLRTVVPQWDAEARKKGLLPAEE
PKAVVAPKAEAK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=441, Percent_Identity=59.6371882086168, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 55069; Mature: 54937

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIAMLGCLSSAAWAEEKPAQTPSLTDKALKHEKLGVVVESANHKFAEKYPLQYNSWKA
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCHHHHCCCCCCCCCC
TSESTDRGSALAADPRYVVLWAGYAFAKDYNKPRGHYYAITDVREILRTGAPKDENDGPQ
CCCCCCCCCEEECCCCEEEEECCHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
PMACWTCKGPDVARLIEEKGERGYFDPKWAKYGAEIVNSIGCADCHDTTSKEFEEGKPAL
CCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHH
RVARPHVLRALETVGWKFEDLDKHGKRTAVCANCHVEYYFKGKTDVTFPWDKGVDVDSIE
HHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECEEEEEECCCCCEECCCCCCCCHHHHH
KYYDEIQFADWTHALSKAPMLKTQHPDFEIWSQGVHGKNGVTCIDCHMPKVKDKDGKVYT
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEE
DHKIGNPFDQFDATCKTCHEQSKETLEKRVKEHKHQVKEAMIRLEDQLVKAHFEAKAAWE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGATQEEMKDILTAIRHAQWRWDYSAAGHGSHMHAPDVMLHVIASGIDRAADARAQLGVV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAKHGVTTPVAIPDISTKEKAQKAIGLDIPKDQAAKDEFLRTVVPQWDAEARKKGLLPAE
HHCCCCCCCEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCC
EPKAVVAPKAEAK
CCCEEECCCCCCC
>Mature Secondary Structure 
ATIAMLGCLSSAAWAEEKPAQTPSLTDKALKHEKLGVVVESANHKFAEKYPLQYNSWKA
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCHHHHCCCCCCCCCC
TSESTDRGSALAADPRYVVLWAGYAFAKDYNKPRGHYYAITDVREILRTGAPKDENDGPQ
CCCCCCCCCEEECCCCEEEEECCHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
PMACWTCKGPDVARLIEEKGERGYFDPKWAKYGAEIVNSIGCADCHDTTSKEFEEGKPAL
CCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHH
RVARPHVLRALETVGWKFEDLDKHGKRTAVCANCHVEYYFKGKTDVTFPWDKGVDVDSIE
HHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECEEEEEECCCCCEECCCCCCCCHHHHH
KYYDEIQFADWTHALSKAPMLKTQHPDFEIWSQGVHGKNGVTCIDCHMPKVKDKDGKVYT
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEE
DHKIGNPFDQFDATCKTCHEQSKETLEKRVKEHKHQVKEAMIRLEDQLVKAHFEAKAAWE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGATQEEMKDILTAIRHAQWRWDYSAAGHGSHMHAPDVMLHVIASGIDRAADARAQLGVV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAKHGVTTPVAIPDISTKEKAQKAIGLDIPKDQAAKDEFLRTVVPQWDAEARKKGLLPAE
HHCCCCCCCEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCC
EPKAVVAPKAEAK
CCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA