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The map label for this gene is kdsB

Identifier: 126207573

GI number: 126207573

Start: 93486

End: 94238

Strand: Direct

Name: kdsB

Synonym: APL_0085

Alternate gene names: 126207573

Gene position: 93486-94238 (Clockwise)

Preceding gene: 126207572

Following gene: 126207574

Centisome position: 4.11

GC content: 46.35

Gene sequence:

>753_bases
ATGAATTTTACTGTGATTATTCCGGCTCGTTACGCTTCAAGCCGCTTACCTCGTAAACCGTTGTTAGATATTGCCGGTAA
ACCGATGATTCAACACGTGTGGGAAAAGGCGCAACAAGCTGGTGCAACACGTGTGATTATTGCGACTGATCATCCGGAGA
TTGAAGCAACTGCTAAAGCATTTGGCGCGGAAGTGTGCATGACTTCGGATCAACATAATTCAGGCACGGAACGTTTAGCG
GAAGTGATTGAAAAGATGCAGATTGCCGATGATGAAATTATCGTGAATGTACAAGGTGATGAGCCGTTGATCCCGCCGGT
GATCGTCTCGCAAGTGGCAGAAAATTTAGATCGTTGCCAAGTGAATATGGCGACATTAGCGGTAAAATTAACCACCAAAG
AAGAACTTTTCAATCCGAATGCCGTCAAAGCGCTGGCGGATAAAAACGGTATGGCACTCTATTTCTCTCGTGCGCCGATT
CCGTTTGCCCGTGATCATTTCGCAGACTGTGATGACGCTTTTGTCGCAAGCCAGAATTACTTACGCCATATCGGTATCTA
CGCATACCGTGCCGGCTTTGTGAAACAATACGTCGCTTGGCAGCCGACCCAATTAGAACAATTGGAATCGTTAGAGCAAC
TACGTGCGTTATGGTACGGAGAAAAAATTCATATCGAACTTGCTAAACAGGCACCTCAAGTGGGAGTGGATACGCAAGAA
GATTTGGAACGTGTTCGCCGAATTCTGGCTTAG

Upstream 100 bases:

>100_bases
TACGGAACATTAGCTTCATTCAAACAAGCGGTTAAATTTCACAAGAATTTTGCCATGTTGATTTAAGCGGATGTTCCGTG
TTTTTTATAAAGGAAATGTT

Downstream 100 bases:

>100_bases
AATCGGCGGAAACAAGCGGTCAAAATTGCAAAAAACTTTGCAAATCAGACCGCTTAATTATCTAGGCTGAGTAGCAACTC
AGTAAATAAAAACGTGCTTA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase 1; CKS 1; CMP-KDO synthase 1

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQQAGATRVIIATDHPEIEATAKAFGAEVCMTSDQHNSGTERLA
EVIEKMQIADDEIIVNVQGDEPLIPPVIVSQVAENLDRCQVNMATLAVKLTTKEELFNPNAVKALADKNGMALYFSRAPI
PFARDHFADCDDAFVASQNYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALWYGEKIHIELAKQAPQVGVDTQE
DLERVRRILA

Sequences:

>Translated_250_residues
MNFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQQAGATRVIIATDHPEIEATAKAFGAEVCMTSDQHNSGTERLA
EVIEKMQIADDEIIVNVQGDEPLIPPVIVSQVAENLDRCQVNMATLAVKLTTKEELFNPNAVKALADKNGMALYFSRAPI
PFARDHFADCDDAFVASQNYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALWYGEKIHIELAKQAPQVGVDTQE
DLERVRRILA
>Mature_250_residues
MNFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQQAGATRVIIATDHPEIEATAKAFGAEVCMTSDQHNSGTERLA
EVIEKMQIADDEIIVNVQGDEPLIPPVIVSQVAENLDRCQVNMATLAVKLTTKEELFNPNAVKALADKNGMALYFSRAPI
PFARDHFADCDDAFVASQNYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALWYGEKIHIELAKQAPQVGVDTQE
DLERVRRILA

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=246, Percent_Identity=61.7886178861789, Blast_Score=321, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB1_ACTP2 (A3MYF7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052798.1
- ProteinModelPortal:   A3MYF7
- SMR:   A3MYF7
- STRING:   A3MYF7
- GeneID:   4850180
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0085
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   FSRAPLP
- PhylomeDB:   A3MYF7
- ProtClustDB:   PRK13368
- BioCyc:   APLE416269:APL_0085-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28023; Mature: 28023

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQQAGATRVIIATDHPEIEATAKA
CCEEEEEECHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
FGAEVCMTSDQHNSGTERLAEVIEKMQIADDEIIVNVQGDEPLIPPVIVSQVAENLDRCQ
HCHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHE
VNMATLAVKLTTKEELFNPNAVKALADKNGMALYFSRAPIPFARDHFADCDDAFVASQNY
EEEHHEEEEEECHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCHHHHHHHHH
LRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALWYGEKIHIELAKQAPQVGVDTQE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCHH
DLERVRRILA
HHHHHHHHHC
>Mature Secondary Structure
MNFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQQAGATRVIIATDHPEIEATAKA
CCEEEEEECHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
FGAEVCMTSDQHNSGTERLAEVIEKMQIADDEIIVNVQGDEPLIPPVIVSQVAENLDRCQ
HCHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHE
VNMATLAVKLTTKEELFNPNAVKALADKNGMALYFSRAPIPFARDHFADCDDAFVASQNY
EEEHHEEEEEECHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCHHHHHHHHH
LRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALWYGEKIHIELAKQAPQVGVDTQE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCHH
DLERVRRILA
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA