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The map label for this gene is murD [H]

Identifier: 126207504

GI number: 126207504

Start: 18128

End: 19438

Strand: Direct

Name: murD [H]

Synonym: APL_0016

Alternate gene names: 126207504

Gene position: 18128-19438 (Clockwise)

Preceding gene: 126207503

Following gene: 126207505

Centisome position: 0.8

GC content: 44.77

Gene sequence:

>1311_bases
ATGCAAAACCAATATAAAGATAAAGTCATTACGGTTATCGGCTTAGGTAAAACAGGTTTATCTTGTGTCGAATTTTTGGC
GGATAAGCAAGCAAAAGTACAAGTGATTGATACCCGCGAAAAGCCGGCGGGTGCCGATCAGCTTCCGGCAGATGTACCGT
TGCATACCGGCGGTTTAAACAGCGAGTGGCTGTTAGCCTCCGATTTAATTGTAATTAGTCCGGGGCTTGCGTTGGCTACA
CCGGAAATTCAACAAGCGATCAACGCCGGTATGGAAGTGGTTGGCGATATCGAATTATTTGTACGTGAGGCGAAAGCGCC
GATTATTGCGATTACCGGTGCAAACGGCAAAAGCACGGTGACGACCTTAGTGACCGAAATGGCAAAACAGGCGGGGATTA
AAGTCGGTATGGGCGGCAATATCGGAGTGCCGGTATTAAGCCTGTTAAACGATGAATATGAATTGTTTGTGTTGGAACTG
TCTAGTTTCCAATTAGAAACCACTTCGTCATTAAATGCGGTGGCAGCAACTATTTTAAATATTAGTGAAGATCATATGGA
TCGTTACAATAACTCGCTGGAAGAATATCGTTTAGCGAAATTGCGTATTTATGACAATGCCGAGAATGTGATTGTAAACG
GTGAAGATAGACTGACTTATCCGCTTGAAACGCAAGCGGTCAAAAAATTGATTCGTTTTGCAGAACATAACGCCGAATAT
ACGATGCGTAACGGCATTTTATATTCGGGCGATGATGCGATTATCCCAACTAAAGAAATGCTGATTTCAGGTCGTCATAA
CGAAATGAATTCATTAGCGGCAATGGCGTTAGCCGAAGCGGCGGGCATTCCGCGTGAAGGAATTGTCAAAGCATTGCAAG
TGTACGGCGGTTTGAATCACCGTTTCCAAGCGGTCAAGACCAATGACGGCGTGCGTTGGGTAAACGACTCAAAAGCAACC
AATGTCGGCAGTACGGTAGCGGCACTCAACGGTTTACAAGTAGCGGGAACGCTTTATTTGTTATTAGGGGGTGACGGTAA
AGCCGCTGATTTTTCCGAGTTAAAACCGCTTATCAATAAACCGAATATCGTTTGTTATTGCTTCGGTCAGGACGGTGCGC
AATTAGCCGCATTATCAAGCCAAAGCGTATTGGTGGAGACTATGCAACAAGCGATTGAACAAATCCGCCCGCAATTGAAA
AAAGGCGATATGGTGTTATTGTCACCGGCGTGCGCAAGTCTCGATCAATTTAAGAACTTTGAAGAACGCGGACAGGTTTT
TGTTAAATTAGCTCAGGAAGTAACCGCTTAA

Upstream 100 bases:

>100_bases
CGTTAAAACTTCGTTAATCATAGGTGGGCTTGTCCCACCTTTTTCGCTTGCAAGCGGTCTGATTTATCCAAATTTTTACA
AGTTATAAAGAGATTCAATT

Downstream 100 bases:

>100_bases
TATGTTAGAAAGATTGAAGACAGAATGGGATAAATGGGTTCGATTAACACCGGCGAATTCGTTATACGATCGTACTTTGC
TTTGGCTGTTTTTCGGCTTA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQNQYKDKVITVIGLGKTGLSCVEFLADKQAKVQVIDTREKPAGADQLPADVPLHTGGLNSEWLLASDLIVISPGLALAT
PEIQQAINAGMEVVGDIELFVREAKAPIIAITGANGKSTVTTLVTEMAKQAGIKVGMGGNIGVPVLSLLNDEYELFVLEL
SSFQLETTSSLNAVAATILNISEDHMDRYNNSLEEYRLAKLRIYDNAENVIVNGEDRLTYPLETQAVKKLIRFAEHNAEY
TMRNGILYSGDDAIIPTKEMLISGRHNEMNSLAAMALAEAAGIPREGIVKALQVYGGLNHRFQAVKTNDGVRWVNDSKAT
NVGSTVAALNGLQVAGTLYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAQLAALSSQSVLVETMQQAIEQIRPQLK
KGDMVLLSPACASLDQFKNFEERGQVFVKLAQEVTA

Sequences:

>Translated_436_residues
MQNQYKDKVITVIGLGKTGLSCVEFLADKQAKVQVIDTREKPAGADQLPADVPLHTGGLNSEWLLASDLIVISPGLALAT
PEIQQAINAGMEVVGDIELFVREAKAPIIAITGANGKSTVTTLVTEMAKQAGIKVGMGGNIGVPVLSLLNDEYELFVLEL
SSFQLETTSSLNAVAATILNISEDHMDRYNNSLEEYRLAKLRIYDNAENVIVNGEDRLTYPLETQAVKKLIRFAEHNAEY
TMRNGILYSGDDAIIPTKEMLISGRHNEMNSLAAMALAEAAGIPREGIVKALQVYGGLNHRFQAVKTNDGVRWVNDSKAT
NVGSTVAALNGLQVAGTLYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAQLAALSSQSVLVETMQQAIEQIRPQLK
KGDMVLLSPACASLDQFKNFEERGQVFVKLAQEVTA
>Mature_436_residues
MQNQYKDKVITVIGLGKTGLSCVEFLADKQAKVQVIDTREKPAGADQLPADVPLHTGGLNSEWLLASDLIVISPGLALAT
PEIQQAINAGMEVVGDIELFVREAKAPIIAITGANGKSTVTTLVTEMAKQAGIKVGMGGNIGVPVLSLLNDEYELFVLEL
SSFQLETTSSLNAVAATILNISEDHMDRYNNSLEEYRLAKLRIYDNAENVIVNGEDRLTYPLETQAVKKLIRFAEHNAEY
TMRNGILYSGDDAIIPTKEMLISGRHNEMNSLAAMALAEAAGIPREGIVKALQVYGGLNHRFQAVKTNDGVRWVNDSKAT
NVGSTVAALNGLQVAGTLYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAQLAALSSQSVLVETMQQAIEQIRPQLK
KGDMVLLSPACASLDQFKNFEERGQVFVKLAQEVTA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=435, Percent_Identity=59.3103448275862, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47020; Mature: 47020

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQYKDKVITVIGLGKTGLSCVEFLADKQAKVQVIDTREKPAGADQLPADVPLHTGGLN
CCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCC
SEWLLASDLIVISPGLALATPEIQQAINAGMEVVGDIELFVREAKAPIIAITGANGKSTV
CCEEEECCEEEECCCCEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
TTLVTEMAKQAGIKVGMGGNIGVPVLSLLNDEYELFVLELSSFQLETTSSLNAVAATILN
HHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCEEEEEEEECCEEEECCCCHHHHHHHHHC
ISEDHMDRYNNSLEEYRLAKLRIYDNAENVIVNGEDRLTYPLETQAVKKLIRFAEHNAEY
CCHHHHHHHCCCHHHHEEEEEEEEECCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCE
TMRNGILYSGDDAIIPTKEMLISGRHNEMNSLAAMALAEAAGIPREGIVKALQVYGGLNH
EEECCEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
RFQAVKTNDGVRWVNDSKATNVGSTVAALNGLQVAGTLYLLLGGDGKAADFSELKPLINK
EEEEEECCCCEEEECCCCCCCCCHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHCC
PNIVCYCFGQDGAQLAALSSQSVLVETMQQAIEQIRPQLKKGDMVLLSPACASLDQFKNF
CCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCH
EERGQVFVKLAQEVTA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNQYKDKVITVIGLGKTGLSCVEFLADKQAKVQVIDTREKPAGADQLPADVPLHTGGLN
CCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCC
SEWLLASDLIVISPGLALATPEIQQAINAGMEVVGDIELFVREAKAPIIAITGANGKSTV
CCEEEECCEEEECCCCEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
TTLVTEMAKQAGIKVGMGGNIGVPVLSLLNDEYELFVLELSSFQLETTSSLNAVAATILN
HHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCEEEEEEEECCEEEECCCCHHHHHHHHHC
ISEDHMDRYNNSLEEYRLAKLRIYDNAENVIVNGEDRLTYPLETQAVKKLIRFAEHNAEY
CCHHHHHHHCCCHHHHEEEEEEEEECCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCE
TMRNGILYSGDDAIIPTKEMLISGRHNEMNSLAAMALAEAAGIPREGIVKALQVYGGLNH
EEECCEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
RFQAVKTNDGVRWVNDSKATNVGSTVAALNGLQVAGTLYLLLGGDGKAADFSELKPLINK
EEEEEECCCCEEEECCCCCCCCCHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHCC
PNIVCYCFGQDGAQLAALSSQSVLVETMQQAIEQIRPQLKKGDMVLLSPACASLDQFKNF
CCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCH
EERGQVFVKLAQEVTA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA