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The map label for this gene is pcnB [H]

Identifier: 126175689

GI number: 126175689

Start: 4092514

End: 4093866

Strand: Direct

Name: pcnB [H]

Synonym: Sbal_3492

Alternate gene names: 126175689

Gene position: 4092514-4093866 (Clockwise)

Preceding gene: 126175688

Following gene: 126175690

Centisome position: 79.82

GC content: 50.04

Gene sequence:

>1353_bases
GTGCCAAGAGATGCCCATACCATCTCGCGCAAACAAATCAGCGAAAATGCCCTTAAGGTATTATACAGACTGAATAAATC
AGGTTTTAAAGCCTATTTAGTCGGCGGCGGTGTACGCGATTTACTCTTAGGGATTGAACCGAAAGATTTCGACGTCGTGA
CCAATGCGACGCCGGAAGAAATCAAAAAACTATTCCGCAACTGCCGAGTCGTTGGTCGTCGTTTCCGTCTGGCCCATATC
GTTTTCGGCCGCGATGTTATCGAAGTGGCGACCTTTCGTGGTCACCATGGCGAAAGCAATGAGAAAGTATCTAAAGCCAA
TGCCGAAGGGCGTTTGCTGCGTGATAACGTTTATGGCGAAATCGACGAAGATGCCGAACGCCGCGACTTTACCGTCAATG
CCCTCTATTACGATATCAGCGATTACTCTATCCGTAGCTACGGCGGCGGTATGACAGATCTTAAAGCTGGCACGCTGAGA
TTAATTGGCGATCCAGAAACCCGTTACCGTGAAGATCCGGTGCGTATGTTAAGGGCGGTGCGTTTCGCCACTAAACTCAA
CATGGGTATCGAAACAGTAACAGCAGCACCGATTAAACAATTAGCGCCACTGTTAAAAGATATTCCCGCAGCACGCATGT
ACGAAGAAGTGCTGAAACTCTTCTTTGCAGGCAAAGCCTTAGCCAACTTCGAACTGATGCAAGAATACAAGCTATTCGCG
CCGCTGTTTCCGCAAGTGGCCGCTCTGCTAAAAGATGATCCTAAAGGCACGACCATGAAAATGGTGCAAGCCATCATGAA
GAGCACAGATCAAAGAGTTAACGAAGATAAGCCGGTGACGCCTTCATTCTTCTATGCTGCGCTTCTCTGGTATCCACTGC
GCCAGCGCGCCGAAGATATTGCCGTTGAAAGTGGCCTGACGTTATACGATGCCTTCTTTGCCGCTATGGGCGATGTGATG
GAGCAGCAATGCCAAACCATTAGCATTCCACGCCGTTTTAGTACACCAGCAAAAGACATTTGGCAGTTACAGTTACGCTT
TGATCGTAGCCAAGGTACTCGCGCCTTTAAACTAATGGAACACCCTAAGTTTAGAGCCGCATACGATTTGCTATTGCTCA
GGGGCGAAGTCGAGGCGGGTAATGTCGCTAAGAGTGCCGCTTGGTGGCAACAGTTTGTTGAAGCCGATGAAACGGAACGC
TTAGCCATTGCGCGTAGCGGCACTAAATCGCCCAATCGCAACCGTAATGCCACTCAACGTCGTCGCCGTCCTCGCGCGCC
GAATGCCGATGGTTCAGCCGCGACGAGCGCACCTAAAACAAGTGGCACGCCAAAAGCGAAAGCGGCTGAATAA

Upstream 100 bases:

>100_bases
ACAGCTATTTGAAGACGCGCCTAAAACGACCGCTCCGACTCAAGCTCAGATTGAACCGACTACGCCTGAACAAAGTTCCG
GTGCGTTAAGCTTAGAGTTA

Downstream 100 bases:

>100_bases
TGACTCGCGTCTACGTCGCATTAGGCGCAAATCTTGAAGATCCCAAGACGCAATTGGACAATGCCGTCACGGCATTGTCC
TCATTAGCCTTAGGCAATAG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHI
VFGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMTDLKAGTLR
LIGDPETRYREDPVRMLRAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFA
PLFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDIAVESGLTLYDAFFAAMGDVM
EQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETER
LAIARSGTKSPNRNRNATQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE

Sequences:

>Translated_450_residues
MPRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHI
VFGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMTDLKAGTLR
LIGDPETRYREDPVRMLRAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFA
PLFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDIAVESGLTLYDAFFAAMGDVM
EQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETER
LAIARSGTKSPNRNRNATQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE
>Mature_449_residues
PRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHIV
FGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMTDLKAGTLRL
IGDPETRYREDPVRMLRAVRFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFAP
LFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDIAVESGLTLYDAFFAAMGDVME
QQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLMEHPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERL
AIARSGTKSPNRNRNATQRRRRPRAPNADGSAATSAPKTSGTPKAKAAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=213, Percent_Identity=29.5774647887324, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI87081691, Length=395, Percent_Identity=58.4810126582278, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=225, Percent_Identity=27.1111111111111, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281360112, Length=290, Percent_Identity=27.2413793103448, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357337, Length=290, Percent_Identity=27.2413793103448, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50766; Mature: 50635

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHH
IKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNVYGE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEECCCCCC
IDEDAERRDFTVNALYYDISDYSIRSYGGGMTDLKAGTLRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCCHHHCCCCCCCCCCCCEEEECCCHHHHCCCHHHHHHHH
RFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFA
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLME
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHHHHEECCCCCCHHHHHHH
HPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGTKSPNRNRNATQR
CCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHH
RRRPRAPNADGSAATSAPKTSGTPKAKAAE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRDAHTISRKQISENALKVLYRLNKSGFKAYLVGGGVRDLLLGIEPKDFDVVTNATPEE
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHH
IKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGESNEKVSKANAEGRLLRDNVYGE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEECCCCCC
IDEDAERRDFTVNALYYDISDYSIRSYGGGMTDLKAGTLRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCCHHHCCCCCCCCCCCCEEEECCCHHHHCCCHHHHHHHH
RFATKLNMGIETVTAAPIKQLAPLLKDIPAARMYEEVLKLFFAGKALANFELMQEYKLFA
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLFPQVAALLKDDPKGTTMKMVQAIMKSTDQRVNEDKPVTPSFFYAALLWYPLRQRAEDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLME
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHHHHEECCCCCCHHHHHHH
HPKFRAAYDLLLLRGEVEAGNVAKSAAWWQQFVEADETERLAIARSGTKSPNRNRNATQR
CCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHH
RRRPRAPNADGSAATSAPKTSGTPKAKAAE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]