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The map label for this gene is gluQ [H]

Identifier: 126175688

GI number: 126175688

Start: 4091391

End: 4092305

Strand: Direct

Name: gluQ [H]

Synonym: Sbal_3491

Alternate gene names: 126175688

Gene position: 4091391-4092305 (Clockwise)

Preceding gene: 126175687

Following gene: 126175689

Centisome position: 79.8

GC content: 50.93

Gene sequence:

>915_bases
ATGGCGACTTTATCCTCCCCAACTTCAATTTATCGCCCCTATGTCGGCCGTTTTGCGCCTTCGCCTTCGGGTGCCTTGCA
CTTTGGCTCGCTTATCGCCGCCCTTGGCAGTTATCTGCGTGCGCGCTCGCAACAGGGTAAATGGTTAATTCGTATTGAAG
ACATCGATCCTCCACGGGAAGTCAAAGGCGCCGCCGATGATATCTTGCGCACCTTAGAAGCCTATGGATTTGAGTGGGAT
GATGTGCCTCTGTATCAGAGCCAGCGCACTGCGGCCTATCAAGCACAAATTGATTTGCTGCTCGCCCAAGATAAAGCCTA
TTTCTGCCAATGTAGTCGCAAGCAAATTCAAGCCATGGGCGGCGTCTACGATGGCCGCTGTCACCACTTGCCACAAACTC
AAGCTACAAATGTGGTTAAGTCGGGGGCGGTGCGGATAGTTAATACCACGCAAGTGCAGCAATTTGACGATAACCTCATG
GGGCATATCAGCGTCGATAGCCATTTTGCCGCCGAGGATTTCATCATCAAACGCAGCGATGGCCTGTACGCCTATCAATT
GGCCGTGGTGCTCGACGATGCCTATCAAGGCATCACTGAAGTGGTTCGAGGTTGTGACTTAATCGAAGCCAGTTGTCGCC
AACTGAGTTTGTATCAAAACTTTGGCTTAGCGGCACCGCAATGGTTGCACTTGCCACTCGCCTGTTTAAGTCAGGGATTT
AAACTCTCTAAACAGAATCATGCGCAGGCGATTGATACGCGTCATCCACAGTCAAGCCTCAATGCGGCGCTGGCATTTTT
AGGCCAACAACCCGTTGAGACCCAAGGTTCTGTGGCACAAATGCTCGCTCAAGCCGTAAATCAGTTTCAATTATCCACTA
TACCGCAACAATCTGAGATACTGATCGAGCCCTGA

Upstream 100 bases:

>100_bases
TTGAAGCCAAGCTCACTCCAATTTACCCCGATTCGCTTTTATCCTTGTATCCCTCTAAAATACGCCCACTTTGACTGACA
CTAGCTGCTAACTCAGTACT

Downstream 100 bases:

>100_bases
TATACTCATTAGGCTGCATTTGGTATATGATAGCCGCCAGATTTTTAATCAAATAACAGATCATATTTCGAGGTGTCCCA
TTTTTCGCCGTATCAGCCAA

Product: glutamate--tRNA ligase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPREVKGAADDILRTLEAYGFEWD
DVPLYQSQRTAAYQAQIDLLLAQDKAYFCQCSRKQIQAMGGVYDGRCHHLPQTQATNVVKSGAVRIVNTTQVQQFDDNLM
GHISVDSHFAAEDFIIKRSDGLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFGLAAPQWLHLPLACLSQGF
KLSKQNHAQAIDTRHPQSSLNAALAFLGQQPVETQGSVAQMLAQAVNQFQLSTIPQQSEILIEP

Sequences:

>Translated_304_residues
MATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPREVKGAADDILRTLEAYGFEWD
DVPLYQSQRTAAYQAQIDLLLAQDKAYFCQCSRKQIQAMGGVYDGRCHHLPQTQATNVVKSGAVRIVNTTQVQQFDDNLM
GHISVDSHFAAEDFIIKRSDGLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFGLAAPQWLHLPLACLSQGF
KLSKQNHAQAIDTRHPQSSLNAALAFLGQQPVETQGSVAQMLAQAVNQFQLSTIPQQSEILIEP
>Mature_303_residues
ATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPREVKGAADDILRTLEAYGFEWDD
VPLYQSQRTAAYQAQIDLLLAQDKAYFCQCSRKQIQAMGGVYDGRCHHLPQTQATNVVKSGAVRIVNTTQVQQFDDNLMG
HISVDSHFAAEDFIIKRSDGLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFGLAAPQWLHLPLACLSQGFK
LSKQNHAQAIDTRHPQSSLNAALAFLGQQPVETQGSVAQMLAQAVNQFQLSTIPQQSEILIEP

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=310, Percent_Identity=32.5806451612903, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI226510925, Length=291, Percent_Identity=55.6701030927835, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788743, Length=260, Percent_Identity=35, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71993394, Length=250, Percent_Identity=33.2, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=33.8028169014084, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24665321, Length=249, Percent_Identity=34.5381526104418, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 33572; Mature: 33441

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPRE
CCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VKGAADDILRTLEAYGFEWDDVPLYQSQRTAAYQAQIDLLLAQDKAYFCQCSRKQIQAMG
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHC
GVYDGRCHHLPQTQATNVVKSGAVRIVNTTQVQQFDDNLMGHISVDSHFAAEDFIIKRSD
CCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCEEEECCC
GLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFGLAAPQWLHLPLACLSQGF
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCC
KLSKQNHAQAIDTRHPQSSLNAALAFLGQQPVETQGSVAQMLAQAVNQFQLSTIPQQSEI
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
LIEP
EECC
>Mature Secondary Structure 
ATLSSPTSIYRPYVGRFAPSPSGALHFGSLIAALGSYLRARSQQGKWLIRIEDIDPPRE
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VKGAADDILRTLEAYGFEWDDVPLYQSQRTAAYQAQIDLLLAQDKAYFCQCSRKQIQAMG
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHC
GVYDGRCHHLPQTQATNVVKSGAVRIVNTTQVQQFDDNLMGHISVDSHFAAEDFIIKRSD
CCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCEEEECCC
GLYAYQLAVVLDDAYQGITEVVRGCDLIEASCRQLSLYQNFGLAAPQWLHLPLACLSQGF
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCC
KLSKQNHAQAIDTRHPQSSLNAALAFLGQQPVETQGSVAQMLAQAVNQFQLSTIPQQSEI
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
LIEP
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]