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The map label for this gene is dsbA [H]

Identifier: 126175595

GI number: 126175595

Start: 3979038

End: 3979793

Strand: Direct

Name: dsbA [H]

Synonym: Sbal_3398

Alternate gene names: 126175595

Gene position: 3979038-3979793 (Clockwise)

Preceding gene: 126175594

Following gene: 126175598

Centisome position: 77.6

GC content: 44.58

Gene sequence:

>756_bases
ATGATTAAACAATTAGCCTTGGCTGTTACCTTAGCGATTGCGCCCTTGAGCACCTTTGCCGCCAATTTTGTTGAAGGCAC
ACATTACACTCAAATCTCGGCCAAAACGCCTAGCAGCGAACCTAAACTGACTGAATTCTATTCATTTTATTGTCATAACT
GCTTCAATATGGAAACCAATTATTTGCCCGATATCAAGGCAAACCTCAATAAACAGATAAGTTTTGACAATAAGCATGTC
GACTTTATGAATAGCGATATTGGCACCGAAGTGATGCGCTCTTTGGCCGTGATCCAAAGCCTTGATAACAAAGACGCCTT
AACCCATGCCATGTTTACCGCAATCCAAGGCACAGAGGGCGCAAACGGTCACGATCACAGTGCACCAGGCCATCAACACG
AGCCTCAAATTAATAGCCGTGATGACATCAAAAAAGTCTTCGCCCAATTTGGTGTCGATGCCGCTAAATACGACGAATTA
GCCGATAGCAAAAGCACCGATGAAAAGCTGGCCCTGTGGCGCACTCAGCAAAATGAATTCAAAATCGACAGCGTCCCCGC
CTTTATCGTGAACGATAAATACGCAGTAAATTTAAACAGCATCAAAACCTTAGATGAGTTGATTGGATTAATTAACTATC
TTGCCGTTGAGAAAGATGCTGCAGCGGCTAAAACCTCATCGGGTGGTAGCCTAGGCTGGTTATTTTTAGTCTTCGCCGCC
GCAATTGGTGTGACGCGTCTGCAACGTCTTAGCTAA

Upstream 100 bases:

>100_bases
TCTAGATTGAAGCTCACTTTATGGCTTCTGTTGGCCGTTAAATATCGACGATACCCACAACTGACACAGTGAATTCAATC
CCACCGAATTTGAGGAAACT

Downstream 100 bases:

>100_bases
ACCAGACACAAGCGTGAATGCAAGGCACCCGCCAGCATAAAAAATGGCCTCCAAATTAGGAGGCCATTTTTATTCGCATT
CGATACAAGCTTTCAATACA

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MIKQLALAVTLAIAPLSTFAANFVEGTHYTQISAKTPSSEPKLTEFYSFYCHNCFNMETNYLPDIKANLNKQISFDNKHV
DFMNSDIGTEVMRSLAVIQSLDNKDALTHAMFTAIQGTEGANGHDHSAPGHQHEPQINSRDDIKKVFAQFGVDAAKYDEL
ADSKSTDEKLALWRTQQNEFKIDSVPAFIVNDKYAVNLNSIKTLDELIGLINYLAVEKDAAAAKTSSGGSLGWLFLVFAA
AIGVTRLQRLS

Sequences:

>Translated_251_residues
MIKQLALAVTLAIAPLSTFAANFVEGTHYTQISAKTPSSEPKLTEFYSFYCHNCFNMETNYLPDIKANLNKQISFDNKHV
DFMNSDIGTEVMRSLAVIQSLDNKDALTHAMFTAIQGTEGANGHDHSAPGHQHEPQINSRDDIKKVFAQFGVDAAKYDEL
ADSKSTDEKLALWRTQQNEFKIDSVPAFIVNDKYAVNLNSIKTLDELIGLINYLAVEKDAAAAKTSSGGSLGWLFLVFAA
AIGVTRLQRLS
>Mature_251_residues
MIKQLALAVTLAIAPLSTFAANFVEGTHYTQISAKTPSSEPKLTEFYSFYCHNCFNMETNYLPDIKANLNKQISFDNKHV
DFMNSDIGTEVMRSLAVIQSLDNKDALTHAMFTAIQGTEGANGHDHSAPGHQHEPQINSRDDIKKVFAQFGVDAAKYDEL
ADSKSTDEKLALWRTQQNEFKIDSVPAFIVNDKYAVNLNSIKTLDELIGLINYLAVEKDAAAAKTSSGGSLGWLFLVFAA
AIGVTRLQRLS

Specific function: Involved in disulfide-bond formation. Required for the functional maturation of secreted virulence factors. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=204, Percent_Identity=26.4705882352941, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 27653; Mature: 27653

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQLALAVTLAIAPLSTFAANFVEGTHYTQISAKTPSSEPKLTEFYSFYCHNCFNMETN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCC
YLPDIKANLNKQISFDNKHVDFMNSDIGTEVMRSLAVIQSLDNKDALTHAMFTAIQGTEG
CCCCHHHCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
ANGHDHSAPGHQHEPQINSRDDIKKVFAQFGVDAAKYDELADSKSTDEKLALWRTQQNEF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHEECCCCCE
KIDSVPAFIVNDKYAVNLNSIKTLDELIGLINYLAVEKDAAAAKTSSGGSLGWLFLVFAA
EECCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHH
AIGVTRLQRLS
HHHHHHHHHCC
>Mature Secondary Structure
MIKQLALAVTLAIAPLSTFAANFVEGTHYTQISAKTPSSEPKLTEFYSFYCHNCFNMETN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCC
YLPDIKANLNKQISFDNKHVDFMNSDIGTEVMRSLAVIQSLDNKDALTHAMFTAIQGTEG
CCCCHHHCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
ANGHDHSAPGHQHEPQINSRDDIKKVFAQFGVDAAKYDELADSKSTDEKLALWRTQQNEF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHEECCCCCE
KIDSVPAFIVNDKYAVNLNSIKTLDELIGLINYLAVEKDAAAAKTSSGGSLGWLFLVFAA
EECCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHH
AIGVTRLQRLS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1631111; 1324389; 10952301; 9149147 [H]